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Institution: Portage Learning
Course Code: BIOL 271
Course Title: Microbiology w/ Lab
Exam: Module 4 Exams
Topic: Microbial Growth, Universal, Selective, And Differential Growth Media
Edition: Updated Version
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QUESTIONS TYPE:
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➢ True Or False:
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,Microbial Growth, Universal, Selective, And Differential Growth Media
Question 1.
True Or False: Differential Media Is Best Suited For Distinguishing Between Two
Similar Species Of Bacteria
A. True
B. False
ANS. A.
Explanation: Differential Media Is Used To Distinguish Between Two Or More Related
Microbes Based On Observable Characteristics Such As Biochemical Reactions.
Question 2.
What Is Selective Media?
ANS. Selective Media Allows For Only The Growth Of Certain Microbes While
Restricting The Growth Of All Others
Explanation: Selective Media Contains Substances That Inhibit Some Microorganisms
While Allowing Specific Microorganisms To Grow.
Question 3.
What Is Differential Media?
ANS. Differential Media Is Used To Distinguish Between Two (Or More) Related
Microbes
Explanation: Differential Media Allows Organisms To Be Distinguished Based On
Differences In Their Biochemical Or Metabolic Activities.
Question 4.
A Researcher Is Asked To Determine Which Of Two Vials Contains E.Coli And Which
Contains Salmonella. Knowing Both Are Gram-Negative While Only One Of Them Is
Capable Of Fermenting Lactose, Which Type Of Media Would Be Best Suited?
,A. Selective And Differential Media
B. Selective Media
C. Growth Media
D. Differential Media
ANS. D.
Explanation: Both Organisms Are Already Known To Be Gram-Negative, So The
Important Distinction Is Their Ability To Ferment Lactose. Differential Media Can
Distinguish Between Organisms Based On This Characteristic.
Question 5.
What Type Of Media Is Best Used To Eliminate Certain Bacteria From Within A Mixed
Culture?
A. Selective Media
B. Differential Media
C. Growth Media
D. Selective And Differential Media
ANS. A.
Explanation: Selective Media Inhibits The Growth Of Certain Bacteria While
Permitting Other Microorganisms To Grow.
Question 6.
A Researcher Is Asked To Determine If A Sample Contains Neisseria Meningtides.
Knowing Neisseria Meningtides Is Slow Growing And Other Foreign Microbes May
Also Be Present In The Culture, Which Type Of Media Would Be Best Suited?
A. Selective Media
B. Selective And Differential Media
C. Differential Media
D. Growth Media
ANS. A.
Explanation: Selective Media Can Restrict The Growth Of Unwanted Microorganisms,
Allowing The Slow-Growing Organism Of Interest To Be Isolated More Effectively.
, Question 7.
Identify The Culture Medium Shown In The Image.
A. Macconkey Agar
B. Chocolate Agar
C. Columbia CNA Agar
D. Eosin Methylene Blue (EMB) Agar
ANS. C.
Explanation: Columbia CNA Agar Contains Colistin And Nalidixic Acid, Which Inhibit
Many Gram-Negative Bacteria And Allow Gram-Positive Bacteria To Grow. It Is
Therefore A Selective Medium For Gram-Positive Organisms.
Question 8.
Identify The Culture Medium Shown In The Image.
A. Sorbitol-Macconkey (SMAC) Agar