ACS BIOCHEMISTRY PRACTICE FINAL BEHARRY
COMPREHENSIVE COURSE REVIEW
◉ thioester bond (1).
Answer: compounds with functional group C-S-CoA-C (eg, in acetyl-
CoA)
◉ zwitterion (3).
Answer: dipolar ion with both positive and negative groups but
overall neutral charge; can act as either an acid or base
◉ Isoelectric point (pI) (chapter 3).
Answer: the characteristic pH at which the net electric charge is zero
◉ SDS - Sodium Dodecyl Sulfate (chap 3).
Answer: detergent used to unfold proteins and give them uniform
negative charge
◉ SDS Page (3).
Answer: chromatography used to separate proteins based on mass.
light proteins travel fast than heavier ones
, ◉ PCR - Polymerase chain reaction (3).
Answer: copies DNA multiple times to increase sample size
◉ Isoelectric focusing (chapt 3).
Answer: procedure used to determine the isoelectric pt (pI) of a
protein. Protein migrates through gel until pH = pI (net charge = 0)
◉ Two-Dimensional Electrophoresis (3).
Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
◉ specific activity (3).
Answer: number of enzyme units per mg of total protein (a measure
of enzyme purity)
◉ activity (3).
Answer: total units of a certain enzyme in a solution
◉ Peptide bonds (chapter 4).
Answer: C-N bond with double bond character due to resonance (C-
N bond cannot rotate, and is planar)
◉ Edman degredation (3).
COMPREHENSIVE COURSE REVIEW
◉ thioester bond (1).
Answer: compounds with functional group C-S-CoA-C (eg, in acetyl-
CoA)
◉ zwitterion (3).
Answer: dipolar ion with both positive and negative groups but
overall neutral charge; can act as either an acid or base
◉ Isoelectric point (pI) (chapter 3).
Answer: the characteristic pH at which the net electric charge is zero
◉ SDS - Sodium Dodecyl Sulfate (chap 3).
Answer: detergent used to unfold proteins and give them uniform
negative charge
◉ SDS Page (3).
Answer: chromatography used to separate proteins based on mass.
light proteins travel fast than heavier ones
, ◉ PCR - Polymerase chain reaction (3).
Answer: copies DNA multiple times to increase sample size
◉ Isoelectric focusing (chapt 3).
Answer: procedure used to determine the isoelectric pt (pI) of a
protein. Protein migrates through gel until pH = pI (net charge = 0)
◉ Two-Dimensional Electrophoresis (3).
Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
◉ specific activity (3).
Answer: number of enzyme units per mg of total protein (a measure
of enzyme purity)
◉ activity (3).
Answer: total units of a certain enzyme in a solution
◉ Peptide bonds (chapter 4).
Answer: C-N bond with double bond character due to resonance (C-
N bond cannot rotate, and is planar)
◉ Edman degredation (3).
