ACS Biochemistry Exam A+ Questions
and Answers Verified Solutions Latest
Update 2026/2027
Question:
Buffer Order and Henderson Hasselbach
Answer:
Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease
Question:
Ramachandran plots
Answer:
Φ = N-R ψ = C-R optimal angle to prevent steric hinderance
Question:
Protein structure of a helix, B sheet, reverse turn
Answer:
a: LEMARKHQ B: VICYFTW RT: PSDNG
Question:
how many amino acids in alpha turn helix
Answer:
3.6
Question:
Super secondary structure
,Answer:
helix turn helix
Question:
parallel vs. antiparallel
Answer:
parallel wedges face same way antiparallel face opposite
Question:
Isoelectric point (pI)
Answer:
pH at which a particular molecule carries no net electrical charge.
Question:
Protein Separation Techniques (ALL)
Answer:
Homogenization Salting Out Affinity Chromatgraphy Ion Exchange Size Exclusion/Gel Filtration
H, S, A, I, SG He said all individuals SinG
Question:
Protein Separation Techniques (H)
Answer:
Homogenization disrupt cells, sonicator, centrifuge
Question:
Protein Separation Techniques (S)
Answer:
Salting out different proteins precipitate out at different salt concentrations
, Question:
Protein Separation Techniques (A)
Answer:
Affinity chromatography add a tag; histidine binds; see how well there is an affinity for the group
Question:
Protein Separation Techniques (I)
Answer:
ion exchange separation based on charge; + proteins stick to - beads
Question:
Protein Separation Techniques (SG)
Answer:
Size Exclusion/Gel Filtration separation based on size
Question:
Cuts (protein separation techniques)
Answer:
cut out what dont need/isnt protein (use 40% concen. then keep liquid; if use 60% concen. then
keep pellet)
Question:
Dialysis
Answer:
protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
and Answers Verified Solutions Latest
Update 2026/2027
Question:
Buffer Order and Henderson Hasselbach
Answer:
Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease
Question:
Ramachandran plots
Answer:
Φ = N-R ψ = C-R optimal angle to prevent steric hinderance
Question:
Protein structure of a helix, B sheet, reverse turn
Answer:
a: LEMARKHQ B: VICYFTW RT: PSDNG
Question:
how many amino acids in alpha turn helix
Answer:
3.6
Question:
Super secondary structure
,Answer:
helix turn helix
Question:
parallel vs. antiparallel
Answer:
parallel wedges face same way antiparallel face opposite
Question:
Isoelectric point (pI)
Answer:
pH at which a particular molecule carries no net electrical charge.
Question:
Protein Separation Techniques (ALL)
Answer:
Homogenization Salting Out Affinity Chromatgraphy Ion Exchange Size Exclusion/Gel Filtration
H, S, A, I, SG He said all individuals SinG
Question:
Protein Separation Techniques (H)
Answer:
Homogenization disrupt cells, sonicator, centrifuge
Question:
Protein Separation Techniques (S)
Answer:
Salting out different proteins precipitate out at different salt concentrations
, Question:
Protein Separation Techniques (A)
Answer:
Affinity chromatography add a tag; histidine binds; see how well there is an affinity for the group
Question:
Protein Separation Techniques (I)
Answer:
ion exchange separation based on charge; + proteins stick to - beads
Question:
Protein Separation Techniques (SG)
Answer:
Size Exclusion/Gel Filtration separation based on size
Question:
Cuts (protein separation techniques)
Answer:
cut out what dont need/isnt protein (use 40% concen. then keep liquid; if use 60% concen. then
keep pellet)
Question:
Dialysis
Answer:
protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
