Leukemias, lymphocytosis, and flow
cytometry Hematology quiz
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____________ is a powerful tool used for analyzing a large
population of single cells - Answer: Flow cytometry
,Types of specimens that are able to be used in flow cytometry
are? - Answer: Samples that are ALIVE! Including tissue samples,
lymph nodes, blood, bone marrow, and CSF
How are tissues prepped for flow cytometry? - Answer: They are
disaggregated to provide single cell suspensions then need to be
incubated with monoclonal antibodies
__________ are usually directly conjugated to fluorochromes -
Answer: Antibodies
What are the four major concepts used in flow cytometry? -
Answer: Fluidics
Optics
Detection
Analysis
What is laminar flow? - Answer: Fluid flowing in parallel layers
without disruption
Describe fluidics - Answer: Isotonic sheath fluid is directed
through a conical chamber of the flow cell under high flow rate
conditions (1-10m/s) to produce a laminar flow system.
, hydrodynamic focusing - Answer: uses flows of denser liquid to
isolate cell stream when sample is introduced
What is captured during optics? - Answer: Light scatter and
fluorescence emission from cells or particles
Wavelength measured with optics? - Answer: 488 nm
FALS approximates what? - Answer: Particle/cell size
What does RALS measure? - Answer: Cytoplasmic complexity and
or granularity
During this process, light waveform is captured and amplified by
photomultiplier (PMT) device as an analog electrical signal -
Answer: Detection
Intensity of signal is proportional to the _________________from
the particle - Answer: Magnitude of light emission photons from
the particle
cytometry Hematology quiz
Comprehensive Questions
(Frequently Tested) and Complete
Solutions Graded A+
Professional Academic Assistance Services
Services Offered
• Proctored Exam Assistance
• Online Class Management (Full Course Support)
• Exam Preparation & Study Materials
• Assignments and Coursework Support
• Essay and Research Paper Writing
• Discussion Posts & Responses
• Editing and Proofreading
• Confidential Academic Consultation
Contact Information
Email:
WhatsApp link: https://wa.me/254704846336
Fast Response | Confidential | Reliable Academic Support
Helping Students Achieve Academic Excellence
____________ is a powerful tool used for analyzing a large
population of single cells - Answer: Flow cytometry
,Types of specimens that are able to be used in flow cytometry
are? - Answer: Samples that are ALIVE! Including tissue samples,
lymph nodes, blood, bone marrow, and CSF
How are tissues prepped for flow cytometry? - Answer: They are
disaggregated to provide single cell suspensions then need to be
incubated with monoclonal antibodies
__________ are usually directly conjugated to fluorochromes -
Answer: Antibodies
What are the four major concepts used in flow cytometry? -
Answer: Fluidics
Optics
Detection
Analysis
What is laminar flow? - Answer: Fluid flowing in parallel layers
without disruption
Describe fluidics - Answer: Isotonic sheath fluid is directed
through a conical chamber of the flow cell under high flow rate
conditions (1-10m/s) to produce a laminar flow system.
, hydrodynamic focusing - Answer: uses flows of denser liquid to
isolate cell stream when sample is introduced
What is captured during optics? - Answer: Light scatter and
fluorescence emission from cells or particles
Wavelength measured with optics? - Answer: 488 nm
FALS approximates what? - Answer: Particle/cell size
What does RALS measure? - Answer: Cytoplasmic complexity and
or granularity
During this process, light waveform is captured and amplified by
photomultiplier (PMT) device as an analog electrical signal -
Answer: Detection
Intensity of signal is proportional to the _________________from
the particle - Answer: Magnitude of light emission photons from
the particle
