University of Colorado Boulder BCHM 4611 Exam 2 |
Questions with 100% Verified Answers | Latest Update
Question:
What determines whether a reaction is spontaneous or not?
Answer:
The sign of the change in free energy (ΔG) of the reaction
Question:
What determines the rate of the reaction?
Answer:
The magnitude of the activation energy (ΔG‡)
Question:
The reaction coordinate for covalent catalysis has...
Answer:
Two peaks, with a trough in between them where the stable intermediate is formed
Question:
In the chymotrypsin reaction, how is the tetrahedral intermediate stabilized?
Answer:
Oxyanion hole stabilization through hydrogen bonding with H's on Ser195 and Gly193
Question:
Competitive inhibitor
Answer:
Competes with the substrate for binding at the enzyme's active site; frequently resemble the
substrate or product of a reaction
Question:
The best competitive inhibitors...
Answer:
Mimic the transition state of the reaction
Question:
Triacylglycerols (triglycerides)
Answer:
A glycerol molecule esterified to three fatty acid molecules; the most common form of fatty acid
storage
, Question:
Integral membrane proteins
Answer:
Have hydrophobic peptide regions embedded in the lipid bilayer and these proteins require strong
detergents to isolate
Question:
Peripheral membrane proteins
Answer:
Associate with the polar head groups of membrane lipids or with the exposed regions of other
membrane proteins; can be isolated with mild salt solutions which will compete to bind with these
regions
Question:
Lipid-linked proteins
Answer:
Insert a hydrophobic anchor into the membrane
Question:
Types of lipid-linked proteins
Answer:
Myristoylated, palmitoylated, prenylated, and GPI-linked
Question:
Myristoylated protein
Answer:
A myristoyl group (C14:0) attached to an N-term Gly via an amide linkage
Question:
Palmitoylated protein
Answer:
A palmitoyl group (C16:0) reversibly attached to a Cys side chain via a thioester linkage
Questions with 100% Verified Answers | Latest Update
Question:
What determines whether a reaction is spontaneous or not?
Answer:
The sign of the change in free energy (ΔG) of the reaction
Question:
What determines the rate of the reaction?
Answer:
The magnitude of the activation energy (ΔG‡)
Question:
The reaction coordinate for covalent catalysis has...
Answer:
Two peaks, with a trough in between them where the stable intermediate is formed
Question:
In the chymotrypsin reaction, how is the tetrahedral intermediate stabilized?
Answer:
Oxyanion hole stabilization through hydrogen bonding with H's on Ser195 and Gly193
Question:
Competitive inhibitor
Answer:
Competes with the substrate for binding at the enzyme's active site; frequently resemble the
substrate or product of a reaction
Question:
The best competitive inhibitors...
Answer:
Mimic the transition state of the reaction
Question:
Triacylglycerols (triglycerides)
Answer:
A glycerol molecule esterified to three fatty acid molecules; the most common form of fatty acid
storage
, Question:
Integral membrane proteins
Answer:
Have hydrophobic peptide regions embedded in the lipid bilayer and these proteins require strong
detergents to isolate
Question:
Peripheral membrane proteins
Answer:
Associate with the polar head groups of membrane lipids or with the exposed regions of other
membrane proteins; can be isolated with mild salt solutions which will compete to bind with these
regions
Question:
Lipid-linked proteins
Answer:
Insert a hydrophobic anchor into the membrane
Question:
Types of lipid-linked proteins
Answer:
Myristoylated, palmitoylated, prenylated, and GPI-linked
Question:
Myristoylated protein
Answer:
A myristoyl group (C14:0) attached to an N-term Gly via an amide linkage
Question:
Palmitoylated protein
Answer:
A palmitoyl group (C16:0) reversibly attached to a Cys side chain via a thioester linkage
