MLT ASCP PRACTICE QUESTIONS & ANSWERS 2026-2027 | LATEST MEDICA… EXAM
P R O F E S S I O N A L P R A C T I C E M AT E R I A L S
MLT ASCP Practice
Questions & Answers
2026-2027 | Latest Medical
Laboratory Technician
Review
Verified Answers Exam Ready With Rationales
98 QUESTIONS
DOCUMENT OVERVIEW
This document provides 98 verified questions with correct answers and explanations, making it a
comprehensive resource for medical laboratory technician review. It covers key concepts in medical
laboratory technology, ensuring thorough understanding and retention of material. Ideal for study and
certification preparation, this resource enables users to solidify their knowledge and enhance their
exam readiness effectively.
CONTENTS
Microbiology Q1–Q15
Clinical Chemistry Q16–Q30
Hematology Q31–Q47
Biochemistry Q48–Q59
Immunology Q60–Q71
Page 1
,Serology Q72–Q84
Pathology Q85–Q98
E XA M Q U EST I O N S
Q1 QUESTION 1 OF 98
A
CORRECT ANSWER
What is the cell that is indicated by the arrow in this field?
A. Red cell fragment
B. Acanthocyte
C. Burr cell
D. Crenated cell
Q2 QUESTION 2 OF 98
D
Page 2
, CORRECT ANSWER
A young man is experiencing difficult breathing after fainting. The physician orders a blood gas analysis
which shows the following results:
pH = 7.25
pCO2 = 62 mmHg
pO2 = 70 mmHg
HCO3 = 23 mEq/L
Which condition is most likely afflicting this patient?
A. metabolic alkalosis
B. metabolic acidosis
C. respiratory alkalosis
D. respiratory acidosis
Q3 QUESTION 3 OF 98
D
CORRECT ANSWER
A positive RPR test and a negative FTA-ABS test is most likely the result of:
A. Primary syphilis
B. Secondary syphilis
C. Latent syphilis
D. False positive reaction
Q4 QUESTION 4 OF 98
D; Anything that can cause significant turbidity in a blood sample, such as high leukocyte count or
lipemia can potentially interfere with the accuracy of a spectrophotometric hemoglobin assay.
CORRECT ANSWER
Hematology
Which of the following may interfere with the accurate measurement of hemoglobin:
A. Leukocytosis
B. EDTA
C. Heparin
D. Leukocytosis and lipemia
Q5 QUESTION 5 OF 98
B; CAMP is the correct answer because the case is typical of Streptococcus agalactiae(Group B strep).
The main presumptive identification test is CAMP. Bile solubility is positive for Streptococcus
pneumonia. Coagulase would be positive for Staphylococcus aureus. PYR is positive for
Streptococcus pyogenes (Group A strep) which is ruled out by the resistance to bacitracin.
Page 3
, CORRECT ANSWER
Microbiology
Spinal fluid cultures on a 3-day-old infant revealed beta-hemolytic, Gram-positive cocci occurring in pairs
and chains. The organism was catalase-negative and bile esculin-negative. Resistance to 0.04 U disk of
bacitracin was noted. The other test necessary to identify this organism is a positive test by:
A. Bile solubility
B. CAMP
C. Coagulase
D. PYR
Q6 QUESTION 6 OF 98
C; Hemoglobin H is a tetramer made of four beta globin chains. Hemoglobin H occurs when there is
very limited alpha chain availability used to make normal hemoglobin A. Hemoglobin H forms in those
affected with alpha thalassemia major as well as in people with the combination of two-gene deletion
alpha thalassemia and hemoglobin Constant Spring.
CORRECT ANSWER
Hematology
Which of the following combination of globin chains comprise Hemoglobin H?
A. 2 alpha, 2 beta
B. 2 alpha, 2 gamma
C. 4 beta chains
D. 4 alpha chains
Q7 QUESTION 7 OF 98
C; Clostridium difficile is a gram-positive, anaerobic, spore-forming bacillus that produces a strong
toxin. C difficile is a common cause of antibiotic-associated diarrhea and pseudomembranous colitis.
CORRECT ANSWER
Microbiology
A recto-sigmoidoscopy revealed pseudomembranes in a patient with severe diarrhea following
prolonged treatment with ampicillin. Which of the following organisms is MOST likely to be isolated?
A. Bacteroides fragilis
B. Clostridium botulinum
C. Clostridium difficile
D. Fusobacterium nucleatum
E. Klebsiella oxytoca
Page 4
P R O F E S S I O N A L P R A C T I C E M AT E R I A L S
MLT ASCP Practice
Questions & Answers
2026-2027 | Latest Medical
Laboratory Technician
Review
Verified Answers Exam Ready With Rationales
98 QUESTIONS
DOCUMENT OVERVIEW
This document provides 98 verified questions with correct answers and explanations, making it a
comprehensive resource for medical laboratory technician review. It covers key concepts in medical
laboratory technology, ensuring thorough understanding and retention of material. Ideal for study and
certification preparation, this resource enables users to solidify their knowledge and enhance their
exam readiness effectively.
CONTENTS
Microbiology Q1–Q15
Clinical Chemistry Q16–Q30
Hematology Q31–Q47
Biochemistry Q48–Q59
Immunology Q60–Q71
Page 1
,Serology Q72–Q84
Pathology Q85–Q98
E XA M Q U EST I O N S
Q1 QUESTION 1 OF 98
A
CORRECT ANSWER
What is the cell that is indicated by the arrow in this field?
A. Red cell fragment
B. Acanthocyte
C. Burr cell
D. Crenated cell
Q2 QUESTION 2 OF 98
D
Page 2
, CORRECT ANSWER
A young man is experiencing difficult breathing after fainting. The physician orders a blood gas analysis
which shows the following results:
pH = 7.25
pCO2 = 62 mmHg
pO2 = 70 mmHg
HCO3 = 23 mEq/L
Which condition is most likely afflicting this patient?
A. metabolic alkalosis
B. metabolic acidosis
C. respiratory alkalosis
D. respiratory acidosis
Q3 QUESTION 3 OF 98
D
CORRECT ANSWER
A positive RPR test and a negative FTA-ABS test is most likely the result of:
A. Primary syphilis
B. Secondary syphilis
C. Latent syphilis
D. False positive reaction
Q4 QUESTION 4 OF 98
D; Anything that can cause significant turbidity in a blood sample, such as high leukocyte count or
lipemia can potentially interfere with the accuracy of a spectrophotometric hemoglobin assay.
CORRECT ANSWER
Hematology
Which of the following may interfere with the accurate measurement of hemoglobin:
A. Leukocytosis
B. EDTA
C. Heparin
D. Leukocytosis and lipemia
Q5 QUESTION 5 OF 98
B; CAMP is the correct answer because the case is typical of Streptococcus agalactiae(Group B strep).
The main presumptive identification test is CAMP. Bile solubility is positive for Streptococcus
pneumonia. Coagulase would be positive for Staphylococcus aureus. PYR is positive for
Streptococcus pyogenes (Group A strep) which is ruled out by the resistance to bacitracin.
Page 3
, CORRECT ANSWER
Microbiology
Spinal fluid cultures on a 3-day-old infant revealed beta-hemolytic, Gram-positive cocci occurring in pairs
and chains. The organism was catalase-negative and bile esculin-negative. Resistance to 0.04 U disk of
bacitracin was noted. The other test necessary to identify this organism is a positive test by:
A. Bile solubility
B. CAMP
C. Coagulase
D. PYR
Q6 QUESTION 6 OF 98
C; Hemoglobin H is a tetramer made of four beta globin chains. Hemoglobin H occurs when there is
very limited alpha chain availability used to make normal hemoglobin A. Hemoglobin H forms in those
affected with alpha thalassemia major as well as in people with the combination of two-gene deletion
alpha thalassemia and hemoglobin Constant Spring.
CORRECT ANSWER
Hematology
Which of the following combination of globin chains comprise Hemoglobin H?
A. 2 alpha, 2 beta
B. 2 alpha, 2 gamma
C. 4 beta chains
D. 4 alpha chains
Q7 QUESTION 7 OF 98
C; Clostridium difficile is a gram-positive, anaerobic, spore-forming bacillus that produces a strong
toxin. C difficile is a common cause of antibiotic-associated diarrhea and pseudomembranous colitis.
CORRECT ANSWER
Microbiology
A recto-sigmoidoscopy revealed pseudomembranes in a patient with severe diarrhea following
prolonged treatment with ampicillin. Which of the following organisms is MOST likely to be isolated?
A. Bacteroides fragilis
B. Clostridium botulinum
C. Clostridium difficile
D. Fusobacterium nucleatum
E. Klebsiella oxytoca
Page 4
