SCYM ASCP CORRECT STUDY QUESTIONS AND
ANSWERS SURE A+
✔✔Photolysis - ✔✔Occurs when energy of the release of electrons breaks a chemical
bond, rendering the molecule non-fluorescent
✔✔Probe Types - ✔✔Antibodies, Viability/DNA Dyes, Physiological Tracking,
Fluorescent Proteins
✔✔Fc Binding - ✔✔Specific binding mediated by the Fc receptor
✔✔Non-Specific Binding - ✔✔Low-affinity binding that results in binding everywhere
✔✔PI Staining - ✔✔Provides a snap-shot of Cell-Cycle in fixed cells, viability marker for
live cells. Intercalates into DNA
✔✔BrdU Staining - ✔✔Stains for timing of the rate of division
✔✔KI67 - ✔✔Indicates the % of cells in Cell-Cycle
✔✔Supravital Dyes - ✔✔For Cell-Cycle determinations in live cells
✔✔ROS - ✔✔Reactive Oxygen
✔✔Rules of Compensation - ✔✔Rule #1: Controls must be at least as bright or brighter
than the sample compensation will be applied to.
Rule #2: Background fluorescence should be the same for the positive and negative
controls.
Rule #3: Compensation controls must exactly match the experimental fluorochrome and
detector settings.
ANSWERS SURE A+
✔✔Photolysis - ✔✔Occurs when energy of the release of electrons breaks a chemical
bond, rendering the molecule non-fluorescent
✔✔Probe Types - ✔✔Antibodies, Viability/DNA Dyes, Physiological Tracking,
Fluorescent Proteins
✔✔Fc Binding - ✔✔Specific binding mediated by the Fc receptor
✔✔Non-Specific Binding - ✔✔Low-affinity binding that results in binding everywhere
✔✔PI Staining - ✔✔Provides a snap-shot of Cell-Cycle in fixed cells, viability marker for
live cells. Intercalates into DNA
✔✔BrdU Staining - ✔✔Stains for timing of the rate of division
✔✔KI67 - ✔✔Indicates the % of cells in Cell-Cycle
✔✔Supravital Dyes - ✔✔For Cell-Cycle determinations in live cells
✔✔ROS - ✔✔Reactive Oxygen
✔✔Rules of Compensation - ✔✔Rule #1: Controls must be at least as bright or brighter
than the sample compensation will be applied to.
Rule #2: Background fluorescence should be the same for the positive and negative
controls.
Rule #3: Compensation controls must exactly match the experimental fluorochrome and
detector settings.
