AMT MLT EXAM BANK 2026 | 2
ACCURATE CURRENTLY TESTING
EXAM VERSIONS WITH VERIFIED
EXAM QUESTIONS AND RATIONALES |
EXPERT VERIFIED FOR GUARANTEED
PASS | LATEST UPDATE
SECTION 1: BLOOD COLLECTION AND PROCESSING (Questions 1-25)
Question 1
A phlebotomist collects a lavender-top tube for a CBC, then a light blue-top tube
for PT/INR. According to CLSI standards, is this order of draw acceptable?
A) Yes, lavender before light blue is the correct order
B) Yes, any order is acceptable as long as tubes are mixed
C) No, the light blue tube must be collected before the lavender tube
D) No, both tubes cannot be drawn in the same session
,,,,answer,,,,: C) No, the light blue tube must be collected before the lavender
tube
Rationale: Order of draw requires the light blue (sodium citrate) tube to be
collected before the lavender (EDTA) tube to prevent contamination with EDTA,
which falsely prolongs coagulation times. The standard order is: blood culture
bottles → light blue → red/serum → green (heparin) → lavender (EDTA) → gray
(fluoride/oxalate) .
,Question 2
The tourniquet should be applied approximately how many inches above the
venipuncture site?
A) 1-2 inches
B) 3-4 inches
C) 5-6 inches
D) 7-8 inches
,,,,answer,,,,: B) 3-4 inches
Rationale: The tourniquet should be applied 3-4 inches above the puncture site to
allow adequate venous filling without causing excessive pressure or discomfort.
Applying too high may reduce effectiveness, while applying too low may interfere
with the puncture site .
Question 3
Which vein is the first choice for routine venipuncture in adult patients?
A) Basilic vein
B) Cephalic vein
C) Dorsal hand vein
D) Median cubital vein
,,,,answer,,,,: D) Median cubital vein
Rationale: The median cubital vein is the preferred site because it is typically large,
well-anchored, less likely to roll, and has fewer adjacent nerves, reducing patient
discomfort .
,Question 4
A lavender-top tube contains which additive and is used for which test?
A) Sodium citrate – coagulation studies
B) EDTA – hematology studies (CBC)
C) Heparin – chemistry tests
D) Sodium fluoride – glucose testing
,,,,answer,,,,: B) EDTA – hematology studies (CBC)
Rationale: Lavender (purple) top tubes contain EDTA (ethylenediaminetetraacetic
acid), which chelates calcium and is used for hematology testing (CBC, blood
smears) because it preserves cell morphology .
Question 5
Which blood collection tube additive acts as an antiglycolytic agent to preserve
glucose?
A) Heparin
B) EDTA
C) Sodium fluoride
D) Sodium citrate
,,,,answer,,,,: C) Sodium fluoride
Rationale: Sodium fluoride inhibits glycolysis by blocking the enzyme enolase,
preserving glucose levels for up to 24 hours. Gray top tubes contain sodium fluoride
and potassium oxalate .
, Question 6
What is the minimum blood-to-anticoagulant ratio required for a light blue top
tube?
A) 1:1
B) 2:1
C) 9:1
D) 4:1
,,,,answer,,,,: C) 9:1
Rationale: Light blue top tubes require a 9:1 blood-to-anticoagulant ratio.
Underfilling or overfilling can alter coagulation test results by affecting calcium
binding and clot formation .
Question 7
A tourniquet should not be left on for longer than which time to avoid
hemoconcentration?
A) 30 seconds
B) 1 minute
C) 2 minutes
D) 5 minutes
,,,,answer,,,,: B) 1 minute
Rationale: Leaving the tourniquet on for more than one minute can cause
hemoconcentration, leading to falsely elevated results for certain analytes (e.g.,
potassium, protein) .
ACCURATE CURRENTLY TESTING
EXAM VERSIONS WITH VERIFIED
EXAM QUESTIONS AND RATIONALES |
EXPERT VERIFIED FOR GUARANTEED
PASS | LATEST UPDATE
SECTION 1: BLOOD COLLECTION AND PROCESSING (Questions 1-25)
Question 1
A phlebotomist collects a lavender-top tube for a CBC, then a light blue-top tube
for PT/INR. According to CLSI standards, is this order of draw acceptable?
A) Yes, lavender before light blue is the correct order
B) Yes, any order is acceptable as long as tubes are mixed
C) No, the light blue tube must be collected before the lavender tube
D) No, both tubes cannot be drawn in the same session
,,,,answer,,,,: C) No, the light blue tube must be collected before the lavender
tube
Rationale: Order of draw requires the light blue (sodium citrate) tube to be
collected before the lavender (EDTA) tube to prevent contamination with EDTA,
which falsely prolongs coagulation times. The standard order is: blood culture
bottles → light blue → red/serum → green (heparin) → lavender (EDTA) → gray
(fluoride/oxalate) .
,Question 2
The tourniquet should be applied approximately how many inches above the
venipuncture site?
A) 1-2 inches
B) 3-4 inches
C) 5-6 inches
D) 7-8 inches
,,,,answer,,,,: B) 3-4 inches
Rationale: The tourniquet should be applied 3-4 inches above the puncture site to
allow adequate venous filling without causing excessive pressure or discomfort.
Applying too high may reduce effectiveness, while applying too low may interfere
with the puncture site .
Question 3
Which vein is the first choice for routine venipuncture in adult patients?
A) Basilic vein
B) Cephalic vein
C) Dorsal hand vein
D) Median cubital vein
,,,,answer,,,,: D) Median cubital vein
Rationale: The median cubital vein is the preferred site because it is typically large,
well-anchored, less likely to roll, and has fewer adjacent nerves, reducing patient
discomfort .
,Question 4
A lavender-top tube contains which additive and is used for which test?
A) Sodium citrate – coagulation studies
B) EDTA – hematology studies (CBC)
C) Heparin – chemistry tests
D) Sodium fluoride – glucose testing
,,,,answer,,,,: B) EDTA – hematology studies (CBC)
Rationale: Lavender (purple) top tubes contain EDTA (ethylenediaminetetraacetic
acid), which chelates calcium and is used for hematology testing (CBC, blood
smears) because it preserves cell morphology .
Question 5
Which blood collection tube additive acts as an antiglycolytic agent to preserve
glucose?
A) Heparin
B) EDTA
C) Sodium fluoride
D) Sodium citrate
,,,,answer,,,,: C) Sodium fluoride
Rationale: Sodium fluoride inhibits glycolysis by blocking the enzyme enolase,
preserving glucose levels for up to 24 hours. Gray top tubes contain sodium fluoride
and potassium oxalate .
, Question 6
What is the minimum blood-to-anticoagulant ratio required for a light blue top
tube?
A) 1:1
B) 2:1
C) 9:1
D) 4:1
,,,,answer,,,,: C) 9:1
Rationale: Light blue top tubes require a 9:1 blood-to-anticoagulant ratio.
Underfilling or overfilling can alter coagulation test results by affecting calcium
binding and clot formation .
Question 7
A tourniquet should not be left on for longer than which time to avoid
hemoconcentration?
A) 30 seconds
B) 1 minute
C) 2 minutes
D) 5 minutes
,,,,answer,,,,: B) 1 minute
Rationale: Leaving the tourniquet on for more than one minute can cause
hemoconcentration, leading to falsely elevated results for certain analytes (e.g.,
potassium, protein) .
