CHT EXAM QUESTIONS AND
CORRECT ANSWERS (VERIFIED
ANSWERS) A GRADE
____ a EDTA is preferred over ACD as an additive for
molecular testing - correct answer- True
____ b- EDTA chelates calcium and is acceptable for CDC
assays - correct answer- False
____ c- EDTA is acceptable for flow cytometry assays -
correct answer- True
____ d- Moderate hemolysis of serum should not
interfere with PRA testing - correct answer-
True
____ e- Plasma is unacceptable for CDC PRA testing -
correct answer- True
,____ f- Heparinized blood is acceptable for molecular
testing - correct answer- True, MOSTLY
____ g- Heparin does not interfere with Taq polymerase -
correct answer- False
____ h- Lithium heparin is an acceptable substitute for
sodium heparin - correct answer- False
____ i- It is acceptable for a red top to be collected from a
patient who is at that moment receiving a dialysis
treatment - correct answer- False
____ j- Blood shipped to the lab for serology testing
should be kept at 4 degrees - correct answer-
False
____ k- Patients receiving long-term steroid treatment
may have insufficient lymphocytes for HLA testing -
correct answer- True
,____ l- Patients receiving blood products should have
their blood collected for HLA typing immediately following
the transfusions to ensure adequate cell numbers -
correct answer- False
____ m- You should ask for the WBC and differential on
leukopenic and pediatric patients to determine how much
blood should be collected for HLA typing - correct
answer- True
____ n- Requisition forms must include name of an
authorized requester - correct answer- True
____ o- Plain red top, EDTA and ACD are all acceptable
samples for ABO blood grouping - correct answer-
True
____ p- Lymphocytes from EBV transformed cell lines
react the same as PBLs in CDC assays - correct
answer- False
, ____ q- The color of the stopper is a universal code for a
vacutainer's contents - correct answer- False
____ r- EDTA blood stored for 1 year at RT is an
acceptable sample - correct answer- False
____ s- Blood spotted on SS 903 cards and stored for 1
year at RT is an acceptable sample - correct
answer- True
____ t- ACD tubes maintain cell viability are better than
heparin during long transit times - correct answer-
True
_____ also inspect laboratories, and because of its
deemed status with other regulatory agencies (OSHA,
HCFA, UNOS, JCAHO), It is compelled to notify those
agencies when an incidence occur. - correct
answer- ASHI
CORRECT ANSWERS (VERIFIED
ANSWERS) A GRADE
____ a EDTA is preferred over ACD as an additive for
molecular testing - correct answer- True
____ b- EDTA chelates calcium and is acceptable for CDC
assays - correct answer- False
____ c- EDTA is acceptable for flow cytometry assays -
correct answer- True
____ d- Moderate hemolysis of serum should not
interfere with PRA testing - correct answer-
True
____ e- Plasma is unacceptable for CDC PRA testing -
correct answer- True
,____ f- Heparinized blood is acceptable for molecular
testing - correct answer- True, MOSTLY
____ g- Heparin does not interfere with Taq polymerase -
correct answer- False
____ h- Lithium heparin is an acceptable substitute for
sodium heparin - correct answer- False
____ i- It is acceptable for a red top to be collected from a
patient who is at that moment receiving a dialysis
treatment - correct answer- False
____ j- Blood shipped to the lab for serology testing
should be kept at 4 degrees - correct answer-
False
____ k- Patients receiving long-term steroid treatment
may have insufficient lymphocytes for HLA testing -
correct answer- True
,____ l- Patients receiving blood products should have
their blood collected for HLA typing immediately following
the transfusions to ensure adequate cell numbers -
correct answer- False
____ m- You should ask for the WBC and differential on
leukopenic and pediatric patients to determine how much
blood should be collected for HLA typing - correct
answer- True
____ n- Requisition forms must include name of an
authorized requester - correct answer- True
____ o- Plain red top, EDTA and ACD are all acceptable
samples for ABO blood grouping - correct answer-
True
____ p- Lymphocytes from EBV transformed cell lines
react the same as PBLs in CDC assays - correct
answer- False
, ____ q- The color of the stopper is a universal code for a
vacutainer's contents - correct answer- False
____ r- EDTA blood stored for 1 year at RT is an
acceptable sample - correct answer- False
____ s- Blood spotted on SS 903 cards and stored for 1
year at RT is an acceptable sample - correct
answer- True
____ t- ACD tubes maintain cell viability are better than
heparin during long transit times - correct answer-
True
_____ also inspect laboratories, and because of its
deemed status with other regulatory agencies (OSHA,
HCFA, UNOS, JCAHO), It is compelled to notify those
agencies when an incidence occur. - correct
answer- ASHI
