1
MLT BOC (ASCP) EXAM REVIEW: MEDICAL LABORATORY
TECHNICIAN (MLT) BOARD OF CERTIFICATION (BOC), 2025-
2026 WITH CORRECT/ACCURATE ANSWERS
The exam is adaptive, consisting of 99% multiple-choice questions over 2.5 hours, and
covers the following major areas:
• Hematology
• Chemistry
• Microbiology
• Blood Banking / Immunohematology
• Urinalysis and Other Body Fluids
• Immunology
• Laboratory Operations
The following results were obtained when testing a 20 he old first time donor: Anti-A -
3+
Anti-B- 0
A1 cells - 1+
B cells - 3+
O cells - 0
pg. 1
,2
The most likely cause of the ABO discrepancy is?
B. Alloantibody reacting with B cell
C. Oh (Bombay)
D. Subgroup of A - Correct Answer: --D
A group A patient needs blood and FFP. The small rural hospital is out of both A blood and
A FFP. Which of the following would be your first to transfuse to this patient?
A. RBC - AB; plasma - AB
B. RBC - AB; plasma - O
C. RBC - O; plasma - AB
D. RBC - O; plasma - O - Correct Answer: --C
For which of the blood groups is it NOT necessary to run an Rh control if you are using
monoclonal/polycolonal blend anti-D?
A. A neg
B. A pos
C. AB neg
D. AB pos - Correct Answer: --B
RBCs which are be tested with antiglobulin reagent are washed to
A. Remove traces of bacterial proteins
B. Wash away traces of free hemoglobin
C. Remove unbound serum globulin
pg. 2
, 3
D. Expose additional antigen sites - Correct Answer: --C
An antibody screen have no reactions at immediate spin or 37C, but showed a 2+ reaction
when antiglobulin reagent was added. He most likely antibodycausing these results would be
anti-
A. I
B. Jka
C. Leb
D. P1 - Correct Answer: --B
Which of the following would cause an individual o be rejected s a blood donor?
A. Donor believes he had some form of hepatitis at age 9
B. Current hgb is 13 g/dL
C. Donor is 65 yo
D. Donor had chicken pox vaccine 3 weeks ago - Correct Answer: --D
Over a 2 week period, the reactions of you QC antibody show a gradual decrease from 2+ to
a very weak positive with your antibody etection cells (screening cells). These results most
likely indicate
A. Acceptable performance of reagent
B. Inappropriate antibody specificity
C. Inconsistent grading of reaction
D. Deterioration of QC antibody - Correct Answer: --D
pg. 3
MLT BOC (ASCP) EXAM REVIEW: MEDICAL LABORATORY
TECHNICIAN (MLT) BOARD OF CERTIFICATION (BOC), 2025-
2026 WITH CORRECT/ACCURATE ANSWERS
The exam is adaptive, consisting of 99% multiple-choice questions over 2.5 hours, and
covers the following major areas:
• Hematology
• Chemistry
• Microbiology
• Blood Banking / Immunohematology
• Urinalysis and Other Body Fluids
• Immunology
• Laboratory Operations
The following results were obtained when testing a 20 he old first time donor: Anti-A -
3+
Anti-B- 0
A1 cells - 1+
B cells - 3+
O cells - 0
pg. 1
,2
The most likely cause of the ABO discrepancy is?
B. Alloantibody reacting with B cell
C. Oh (Bombay)
D. Subgroup of A - Correct Answer: --D
A group A patient needs blood and FFP. The small rural hospital is out of both A blood and
A FFP. Which of the following would be your first to transfuse to this patient?
A. RBC - AB; plasma - AB
B. RBC - AB; plasma - O
C. RBC - O; plasma - AB
D. RBC - O; plasma - O - Correct Answer: --C
For which of the blood groups is it NOT necessary to run an Rh control if you are using
monoclonal/polycolonal blend anti-D?
A. A neg
B. A pos
C. AB neg
D. AB pos - Correct Answer: --B
RBCs which are be tested with antiglobulin reagent are washed to
A. Remove traces of bacterial proteins
B. Wash away traces of free hemoglobin
C. Remove unbound serum globulin
pg. 2
, 3
D. Expose additional antigen sites - Correct Answer: --C
An antibody screen have no reactions at immediate spin or 37C, but showed a 2+ reaction
when antiglobulin reagent was added. He most likely antibodycausing these results would be
anti-
A. I
B. Jka
C. Leb
D. P1 - Correct Answer: --B
Which of the following would cause an individual o be rejected s a blood donor?
A. Donor believes he had some form of hepatitis at age 9
B. Current hgb is 13 g/dL
C. Donor is 65 yo
D. Donor had chicken pox vaccine 3 weeks ago - Correct Answer: --D
Over a 2 week period, the reactions of you QC antibody show a gradual decrease from 2+ to
a very weak positive with your antibody etection cells (screening cells). These results most
likely indicate
A. Acceptable performance of reagent
B. Inappropriate antibody specificity
C. Inconsistent grading of reaction
D. Deterioration of QC antibody - Correct Answer: --D
pg. 3
