1
MLT ASCP PRACTICE EXAM QUESTIONS: MEDICAL
LABORATORY TECHNICIAN CERTIFIED BY THE AMERICAN
SOCIETY FOR CLINICAL PATHOLOGY (ASCP), 2025-2026 WITH
CORRECT/ACCURATE ANSWERS
AMERICAN SOCIETY FOR CLINICAL PATHOLOGY (ASCP)
Standard precautions apply to all of the following except:
a. Blood
b. Cerebrospinal fluid
c. Semen
d. Concentrated acids - Correct Answer: --d. Concentrated acids >>>
Standard precautions are a set of infection control practices that apply to all patients,
regardless of their presumed infection status.
They are designed to protect against potential exposure to infectious agents in biological
materials.
Standard precautions apply to blood, all body fluids, secretions, and excretions (except
sweat), non-intact skin, and mucous membranes.
The most important practice in preventing the spread of disease is: a. Wearing masks
during patient contact
pg. 1
,2
d. Identifying specimens from known or suspected HIV- and HBV-infected patients with a
red label - Correct Answer: --b. Proper hand washing >>> Proper hand washing is widely
recognized by major health organizations, including the Centers for Disease Control and
Prevention (CDC) and the World Health Organization (WHO), as the most effective and
simplest practice for preventing the spread of infectious diseases. It works by physically
removing germs from the hands, which are a primary vehicle for transmitting pathogens from
person to person and from surfaces to people.
The appropriate dilution of bleach to use in laboratory disinfection is: a. 1:2
b. 1:5
c. 1:10
d. 1:100 - Correct Answer: --d. 1:10 >>> A 1:10 dilution (1 part bleach to 9 parts water) of
household bleach (typically 5.25%–6.15% sodium hypochlorite) is the standard
recommendation for decontaminating surfaces and spills of blood or other potentially
infectious materials. This creates a solution with approximately 5,000 ppm of available
chlorine, which is effective for most laboratory pathogens.
For routine, non-biohazard disinfection of nonporous surfaces, a less concentrated 1:100
dilution may be acceptable. However, the 1:10 solution is the standard for blood and body
fluid spills.
The solution must be prepared fresh daily, as it loses its effectiveness over time.
pg. 2
, 3
How frequently should fire alarms and sprinkler systems be tested? a. Weekly
b. Monthly
c. Quarterly
d. Annually - Correct Answer: --c. Quarterly
Where should alcohol and other flammable chemicals be stored?
a. In an approved safety can or storage cabinet away from heat sources
b. Under a hood and arranged alphabetically for ease of identification in an emergency
c. In a refrigerator at 2 C to 8 C to reduce volatilization
d. On a low shelf in an area protected from light - Correct Answer: --a. In an approved
safety can or storage cabinet away from heat sources
The most frequent cause of needle punctures is: a. Patient
movement during venipuncture
b. Improper disposal of phlebotomy equipment
c. Inattention during removal of needle after venipuncture
d. Failure to attach needle firmly to syringe or tube holder - Correct Answer: --b. Improper
disposal of phlebotomy equipment
pg. 3
MLT ASCP PRACTICE EXAM QUESTIONS: MEDICAL
LABORATORY TECHNICIAN CERTIFIED BY THE AMERICAN
SOCIETY FOR CLINICAL PATHOLOGY (ASCP), 2025-2026 WITH
CORRECT/ACCURATE ANSWERS
AMERICAN SOCIETY FOR CLINICAL PATHOLOGY (ASCP)
Standard precautions apply to all of the following except:
a. Blood
b. Cerebrospinal fluid
c. Semen
d. Concentrated acids - Correct Answer: --d. Concentrated acids >>>
Standard precautions are a set of infection control practices that apply to all patients,
regardless of their presumed infection status.
They are designed to protect against potential exposure to infectious agents in biological
materials.
Standard precautions apply to blood, all body fluids, secretions, and excretions (except
sweat), non-intact skin, and mucous membranes.
The most important practice in preventing the spread of disease is: a. Wearing masks
during patient contact
pg. 1
,2
d. Identifying specimens from known or suspected HIV- and HBV-infected patients with a
red label - Correct Answer: --b. Proper hand washing >>> Proper hand washing is widely
recognized by major health organizations, including the Centers for Disease Control and
Prevention (CDC) and the World Health Organization (WHO), as the most effective and
simplest practice for preventing the spread of infectious diseases. It works by physically
removing germs from the hands, which are a primary vehicle for transmitting pathogens from
person to person and from surfaces to people.
The appropriate dilution of bleach to use in laboratory disinfection is: a. 1:2
b. 1:5
c. 1:10
d. 1:100 - Correct Answer: --d. 1:10 >>> A 1:10 dilution (1 part bleach to 9 parts water) of
household bleach (typically 5.25%–6.15% sodium hypochlorite) is the standard
recommendation for decontaminating surfaces and spills of blood or other potentially
infectious materials. This creates a solution with approximately 5,000 ppm of available
chlorine, which is effective for most laboratory pathogens.
For routine, non-biohazard disinfection of nonporous surfaces, a less concentrated 1:100
dilution may be acceptable. However, the 1:10 solution is the standard for blood and body
fluid spills.
The solution must be prepared fresh daily, as it loses its effectiveness over time.
pg. 2
, 3
How frequently should fire alarms and sprinkler systems be tested? a. Weekly
b. Monthly
c. Quarterly
d. Annually - Correct Answer: --c. Quarterly
Where should alcohol and other flammable chemicals be stored?
a. In an approved safety can or storage cabinet away from heat sources
b. Under a hood and arranged alphabetically for ease of identification in an emergency
c. In a refrigerator at 2 C to 8 C to reduce volatilization
d. On a low shelf in an area protected from light - Correct Answer: --a. In an approved
safety can or storage cabinet away from heat sources
The most frequent cause of needle punctures is: a. Patient
movement during venipuncture
b. Improper disposal of phlebotomy equipment
c. Inattention during removal of needle after venipuncture
d. Failure to attach needle firmly to syringe or tube holder - Correct Answer: --b. Improper
disposal of phlebotomy equipment
pg. 3
