MCB160L FINAL
Study online at https://quizlet.com/_jlbihe
1. Chromatin identify: Nucleo- assays for transposase accessible chromatin (AATC-seq)
some-free
2. Yeast 2-hybrid: false posi- autoactivation
tive
3. SELEX determine the binding site of a transcription factor through re-
peated incubation
4. Ubiquitin small peptide
5. what does ubiquitin do? identify interactions between a membrane bound
protein and other proteins in a yeast-based assay
6. condition altered/expand- TF binds to one promoter- six in absence of histidine
ed binding
7. HOT region
8. Tandem affinity purification isolate and identify protein complexes in native state
(TAP)
9. How many steps in TAP tag- 2 steps
ging
10. What tag is used for purifi- Calmodulin binding peptide
cation (TAP)
11. Chromosome conforma- inter-chromosomal and intra chromosomal interactions
tion capture
12. In vivo- is TF binding to ChIP-seq
cis-element?
13. protein binding microarray
, MCB160L FINAL
Study online at https://quizlet.com/_jlbihe
Any- is TF binding to cis-ele-
ment?
14. Homologous recombina- delete gene of interest
tion based mutagenesis
15. signature tagged mutagen- identify mutant from mixed pool
esis
16. yeast one hybrid Bait fused to a reporter gene
17. Tandem Affinity Purifica- TEV protease cleavage
tion
18. TAP tagging
19. Sequence tagging mutage-
nesis
20. Yeast Two Hybrid Bait fused to Gal4 DNA binding
domain
21. ubiquitin yeast two hybrid
22. can TF bind to sites w multi- yes
ple affinity
23. prey
24. bait
25. What is this image? position weight matrix
Study online at https://quizlet.com/_jlbihe
1. Chromatin identify: Nucleo- assays for transposase accessible chromatin (AATC-seq)
some-free
2. Yeast 2-hybrid: false posi- autoactivation
tive
3. SELEX determine the binding site of a transcription factor through re-
peated incubation
4. Ubiquitin small peptide
5. what does ubiquitin do? identify interactions between a membrane bound
protein and other proteins in a yeast-based assay
6. condition altered/expand- TF binds to one promoter- six in absence of histidine
ed binding
7. HOT region
8. Tandem affinity purification isolate and identify protein complexes in native state
(TAP)
9. How many steps in TAP tag- 2 steps
ging
10. What tag is used for purifi- Calmodulin binding peptide
cation (TAP)
11. Chromosome conforma- inter-chromosomal and intra chromosomal interactions
tion capture
12. In vivo- is TF binding to ChIP-seq
cis-element?
13. protein binding microarray
, MCB160L FINAL
Study online at https://quizlet.com/_jlbihe
Any- is TF binding to cis-ele-
ment?
14. Homologous recombina- delete gene of interest
tion based mutagenesis
15. signature tagged mutagen- identify mutant from mixed pool
esis
16. yeast one hybrid Bait fused to a reporter gene
17. Tandem Affinity Purifica- TEV protease cleavage
tion
18. TAP tagging
19. Sequence tagging mutage-
nesis
20. Yeast Two Hybrid Bait fused to Gal4 DNA binding
domain
21. ubiquitin yeast two hybrid
22. can TF bind to sites w multi- yes
ple affinity
23. prey
24. bait
25. What is this image? position weight matrix
