SCYM (ASCP) EXAM(ACTUAL EXAM) WITH
CORRECT ACTUAL QUESTIONS AND
CORRECTLY WELL DEFINED ANSWERS LATEST
ALREADY GRADED A+ 2026
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Terms in this set (133)
Hydrodynamic Focusing Most modern flow cytometers tightly position the
sample for optical analysis via hydrodynamic
focusing. Here, a carrier fluid called the sheath
fluid is used to position the sample of cells into a
single file for optical interrogation.
Hydordynamic focusing and sheath The central stream (sample stream) is focused
fluids and surrounded by the secondary slower stream
(sheath fluid). The shape and size of the flow cell
is crucial to hydrodynamic focusing, and
traditionally the cell is nozzle shaped. ... In a flow
cytometer, the sheath fluid pressure is constant
while the sample fluid is adjusted
Sample Pressure and the Sheath The difference between the sample pressure and
Pressure the sheath pressure is the differential pressure.
This controls the width of the core stream and
the total number of cells passing the laser
intercept.
,differential pressure based flow Differential pressure based flow cytometers
cytometers currently dominate the market. These systems
have two pressure regulators. The first is at a
constant pressure that sets how fast the fluids
runs at. The second is regulated by the
investigator (like on this LSR-II control panel).
Generation of differential pressure Low differential pressure allows the cells to move
(syringe pump, pressure based) past the interrogation point one at a time. .... One
kind involves generating pressure using a pump
and regulator system ... Differential pressure
based fluidic system. ... peristaltic and/or syringe
pumps to deliver the sample into the instrument.
Characterization of syringe-pump- In syringe-pump-driven microfluidic systems,
driven induced pressure pressure fluctuations are observed in an elastic
microchannel. The syringe pump is driven by an
electrical stepper motor, from which mechanical
oscillations are expected to generate flow-rate
fluctuations and in turn leads to the pressure
fluctuations in the channel flow.
Optical Filters Filters are pieces of glass coated on both sides
that allow light of a certain collection, or band, of
wavelengths to pass through while absorbing or
interfering with photons of other wavelengths.
These come in bandpass, longpass, and
shortpass flavors
, Band Pass Optical Filter A filter that allows light between a set wavelength
to pass through and reflects light above and
below the set wavelength. For example, a
bandpass filter with a wavelength of 550/40nm
would allow light between 530nm and 570nm to
pass through, but reflect light below 530nm and
above 570nm.
Longpass Filter wavelength above 650nM
Shortpass Filter wavelength below 488nM
dichroics mirrors Dichroic mirrors can block light by phased
reflection allowing certain light to pass through
and interfering with other wavelengths. For
example, a 500LP dichroic mirror would transmit
light above 500 nm and reflect the light below
500 nm in a different direction. A 525SP dichroic
mirror would transmit all light below 525 nm and
reflect all light above 525 nm in a different
direction. These dichroic mirrors are critical in the
directing and capturing of light by the detectors.
neutral density filter filter that reduces or modifies the intensity of all
wavelengths, or colors, of light equally, giving no
changes in hue of color rendition
CORRECT ACTUAL QUESTIONS AND
CORRECTLY WELL DEFINED ANSWERS LATEST
ALREADY GRADED A+ 2026
Save
Terms in this set (133)
Hydrodynamic Focusing Most modern flow cytometers tightly position the
sample for optical analysis via hydrodynamic
focusing. Here, a carrier fluid called the sheath
fluid is used to position the sample of cells into a
single file for optical interrogation.
Hydordynamic focusing and sheath The central stream (sample stream) is focused
fluids and surrounded by the secondary slower stream
(sheath fluid). The shape and size of the flow cell
is crucial to hydrodynamic focusing, and
traditionally the cell is nozzle shaped. ... In a flow
cytometer, the sheath fluid pressure is constant
while the sample fluid is adjusted
Sample Pressure and the Sheath The difference between the sample pressure and
Pressure the sheath pressure is the differential pressure.
This controls the width of the core stream and
the total number of cells passing the laser
intercept.
,differential pressure based flow Differential pressure based flow cytometers
cytometers currently dominate the market. These systems
have two pressure regulators. The first is at a
constant pressure that sets how fast the fluids
runs at. The second is regulated by the
investigator (like on this LSR-II control panel).
Generation of differential pressure Low differential pressure allows the cells to move
(syringe pump, pressure based) past the interrogation point one at a time. .... One
kind involves generating pressure using a pump
and regulator system ... Differential pressure
based fluidic system. ... peristaltic and/or syringe
pumps to deliver the sample into the instrument.
Characterization of syringe-pump- In syringe-pump-driven microfluidic systems,
driven induced pressure pressure fluctuations are observed in an elastic
microchannel. The syringe pump is driven by an
electrical stepper motor, from which mechanical
oscillations are expected to generate flow-rate
fluctuations and in turn leads to the pressure
fluctuations in the channel flow.
Optical Filters Filters are pieces of glass coated on both sides
that allow light of a certain collection, or band, of
wavelengths to pass through while absorbing or
interfering with photons of other wavelengths.
These come in bandpass, longpass, and
shortpass flavors
, Band Pass Optical Filter A filter that allows light between a set wavelength
to pass through and reflects light above and
below the set wavelength. For example, a
bandpass filter with a wavelength of 550/40nm
would allow light between 530nm and 570nm to
pass through, but reflect light below 530nm and
above 570nm.
Longpass Filter wavelength above 650nM
Shortpass Filter wavelength below 488nM
dichroics mirrors Dichroic mirrors can block light by phased
reflection allowing certain light to pass through
and interfering with other wavelengths. For
example, a 500LP dichroic mirror would transmit
light above 500 nm and reflect the light below
500 nm in a different direction. A 525SP dichroic
mirror would transmit all light below 525 nm and
reflect all light above 525 nm in a different
direction. These dichroic mirrors are critical in the
directing and capturing of light by the detectors.
neutral density filter filter that reduces or modifies the intensity of all
wavelengths, or colors, of light equally, giving no
changes in hue of color rendition
