Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base
ANSWER
Anti-codon
What genes would be screened in a breast cancer panel?
ANSWER
HER2, ERBB2, BRCA1
According to Chargaff's rule of base pairing, adenine pairs with:
ANSWER
Thymine
Next Generation Sequencing uses:
ANSWER
Short sequence reads
Purines and pyrimidines differ from each other in that:
ANSWER
Purines have two rings; pyrimidines have one ring
1
,The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine
ANSWER
Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs
ANSWER
1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not depend
on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule
ANSWER
Nucleotide sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
2
,Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau
ANSWER
Initiation, exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC
ANSWER
3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body movements
and depression comes to the physician's office for a follow-up visit. The physician suspects a
single-gene disorder may be the cause of those clinical manifestations. A blood specimen
was then sent to your clinical laboratory for mutation screening in the Huntington gene.
Testing with standard PCR indicates that patient has Huntington Disease, HD. Which of the
following would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene
ANSWER
85 CAG repeats in the Huntington gene
Which two HPV types are responsible for most cases of cervical cancer?
3
, 16 and 18
31 and 59
16 and 58
44 and 59
ANSWER
16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase
ANSWER
Helicase
Mutation in what gene is associated with Fragile X syndrome?
ANSWER
FMR1
Mantle cell lymphoma (MCL) is caused by what translocation?
ANSWER
t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity":
ANSWER
Pol α
4
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base
ANSWER
Anti-codon
What genes would be screened in a breast cancer panel?
ANSWER
HER2, ERBB2, BRCA1
According to Chargaff's rule of base pairing, adenine pairs with:
ANSWER
Thymine
Next Generation Sequencing uses:
ANSWER
Short sequence reads
Purines and pyrimidines differ from each other in that:
ANSWER
Purines have two rings; pyrimidines have one ring
1
,The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine
ANSWER
Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs
ANSWER
1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not depend
on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule
ANSWER
Nucleotide sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
2
,Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau
ANSWER
Initiation, exponential, plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC
ANSWER
3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body movements
and depression comes to the physician's office for a follow-up visit. The physician suspects a
single-gene disorder may be the cause of those clinical manifestations. A blood specimen
was then sent to your clinical laboratory for mutation screening in the Huntington gene.
Testing with standard PCR indicates that patient has Huntington Disease, HD. Which of the
following would be consistent with this diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene
ANSWER
85 CAG repeats in the Huntington gene
Which two HPV types are responsible for most cases of cervical cancer?
3
, 16 and 18
31 and 59
16 and 58
44 and 59
ANSWER
16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase
ANSWER
Helicase
Mutation in what gene is associated with Fragile X syndrome?
ANSWER
FMR1
Mantle cell lymphoma (MCL) is caused by what translocation?
ANSWER
t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity":
ANSWER
Pol α
4
