you receive a specimen in a lavender top vacutainer tube (EDTA). Test requires specimen be
in a yellow top ACD vacutainer tube. What should you do?
ANSWER
follow lab protocol for accepting and rejecting specimens
best way to store RNA for longer than a month?
ANSWER
ethanol, at -20°C or -80°C
liquid pap
ANSWER
used for HPV testing
peripheral blood
ANSWER
- in EDTA, ACD, heparin (some circ).
- clotted/hemolyzed blood very rarely acceptable
nasopharyngeal swab
ANSWER
FluA/B/RSV
CLIA (Clinical Laboratory Improvement Amendments)
1
, ANSWER
passed by Congress to establish quality testing standards to ensure consistent patient test
results
bone marrow
ANSWER
- analyzed with FISH
- requires high resolution methods (HLA typing)
- store at 4C
solid tissue
ANSWER
if not processed immediately, should be snap frozen in liquid nitrogen
paraffin-embedded tissues
ANSWER
- generally yield lower quality DNA and RNA
- PCR and RT-PCR are routinely performed on these samples (not southern and northern
blot)
brown top collection tube
ANSWER
- sodium heparin
- cytogenic studies, molecular studies
PPT Tube
ANSWER
- black 'tiger' pattern
2
in a yellow top ACD vacutainer tube. What should you do?
ANSWER
follow lab protocol for accepting and rejecting specimens
best way to store RNA for longer than a month?
ANSWER
ethanol, at -20°C or -80°C
liquid pap
ANSWER
used for HPV testing
peripheral blood
ANSWER
- in EDTA, ACD, heparin (some circ).
- clotted/hemolyzed blood very rarely acceptable
nasopharyngeal swab
ANSWER
FluA/B/RSV
CLIA (Clinical Laboratory Improvement Amendments)
1
, ANSWER
passed by Congress to establish quality testing standards to ensure consistent patient test
results
bone marrow
ANSWER
- analyzed with FISH
- requires high resolution methods (HLA typing)
- store at 4C
solid tissue
ANSWER
if not processed immediately, should be snap frozen in liquid nitrogen
paraffin-embedded tissues
ANSWER
- generally yield lower quality DNA and RNA
- PCR and RT-PCR are routinely performed on these samples (not southern and northern
blot)
brown top collection tube
ANSWER
- sodium heparin
- cytogenic studies, molecular studies
PPT Tube
ANSWER
- black 'tiger' pattern
2
