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Exam (elaborations)

Ascp Mb Exam Prep |Questions And Verified Answers|Comprehensive Study Guide|A+ Study Resource|Brand New 2026/2027 Update

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ASCP MB EXAM PREP |QUESTIONS AND VERIFIED ANSWERS|COMPREHENSIVE STUDY GUIDE|A+ STUDY RESOURCE|BRAND NEW 2026/2027 UPDATE

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How are nucleotides joined together?

ANSWER

condensation



What does mRNA do?

ANSWER

carries code out of cell.

code for protein structure.

DNA to ribosomes

end of transcription start of translation



What does tRNA do?

ANSWER

decodes mRNA. Matches amino acids to mRNA code. Protein synthesis



What does rRNA do?

ANSWER

part of ribosome structure

most abundant RNA

coordinated coupling of tRNA to mRNA codons



Feedback Inhibition

ANSWER


1

,end product of pathway is noncompetitive inhibitor

binds to allosteric site to slow down rxn b/c too much product



Endonucleases

ANSWER

cleaves phoshpodiester bonds w/in chain

recognition site is palindromic sequence

cleaves unevenly, leaves sticky ends

(restriction enzymes)\



Exonucleases

ANSWER

degrades nucleic acids by removing one terminal nucleotide at a time

cleaves phosphodiester bond at end of chain

5' ---> 3' and 3' ---> 5'



ORI sites

ANSWER

origin of replication on chromosome

triggers DNA replication



DNA polymerase III

ANSWER

copies DNA by reading existing strand, builds new comp. strand

always adds to 3' end

can't start new strand on its own (ORI sites)




2

, gyrase

ANSWER

unwinds DNA in front of fork



DNA polymerase I

ANSWER

removes RNA nucleotides form RNA primers and replaces with DNA

(makes sure primers that start replication are removed)



helicase

ANSWER

breaks H-bonds of double helix



topoisomerase

ANSWER

breaks/ reseals DNA backbone to release tension caused by twisting

(DNA gyrase)



primase

ANSWER

(RNA pol)

primers, small RNA, serves as starting points for copying DNA

helper enzyme for DNA pol III



single-strand DNA-binding proteins (SSBPs)

ANSWER

hold DNA open during replication


3

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