UTA Microbiology Exam 3 QUESTIONS
AND VERIFIED CORRECT SOLUTIONS ||
100% GUARANTEED PASS BRAND NEW
VERSION
4 Basic Steps of binary fission -ANSWER✅✅✅✅1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly -ANSWER✅✅✅✅FtsZ assembles Z ring to form divisome
Generation Time -ANSWER✅✅✅✅time it takes to double the population
Lag Phase -ANSWER✅✅✅✅Cells adjust to culture medium; no change in population
What factors determine the duration of the lag phase? -ANSWER✅✅✅✅Genetic
makeup, media composition, initial inoculum size
During which phase of the growth curve is it most appropriate for the use of antibiotics?
-ANSWER✅✅✅✅Log phase
Following the death phase of the growth curve, ____ cells are considered the surviving
cells & are considered clinically important due to their association with chronic
infections, such as Tuberculosis. -ANSWER✅✅✅✅Persisters
Log Phase -ANSWER✅✅✅✅Binary fission occurs; cell replication > cell death
Stationary Phase -ANSWER✅✅✅✅Resources become depleted; cell respiration=cell
death
Death Phase -ANSWER✅✅✅✅Endospores can form; cell replication < cell death
Close System Cultures -ANSWER✅✅✅✅Have finite resources
Open System Cultures -ANSWER✅✅✅✅-Nutrients and air are replenished
-Dead cells and waste are removed
, -Beneficial for industrial microbiology
Microscopic Cell Count -ANSWER✅✅✅✅Cells are counted under a microscope using
a laser etched slide
In using the direct microscopic cell count method, you will be able to distinguish
between live vs dead cells. -ANSWER✅✅✅✅False
Fluorescence Staining -ANSWER✅✅✅✅Cells are counted under a microscope or flow
cytometer
In fluorescent staining, the end product results in live cells staining ___________? -
ANSWER✅✅✅✅Green
In fluorescent staining, the end product results in dead cells staining ___________? -
ANSWER✅✅✅✅Red
Coulter counting -ANSWER✅✅✅✅Detects electrical resistance change due to cell
density
Viable Cell Counts -ANSWER✅✅✅✅Samples are diluted and grown on solid media
What type of cells are counted when using the viable plate count method? -
ANSWER✅✅✅✅Living
Countable Range -ANSWER✅✅✅✅30-300 CFU/ml
Optical Density -ANSWER✅✅✅✅-Measured with spectrophotometer
-Light is passed thru culture and measured on other side
Which method of measuring growth is considered an indirect approach? -
ANSWER✅✅✅✅Optical density
Which method of measuring growth can be used on living or dead cells? -
ANSWER✅✅✅✅Fluorescent stain
How is the pour plate technique set up? -ANSWER✅✅✅✅The sample is mixed with a
warm agar ranging from 45-50 C, poured onto sterile plated and swirled to mix
How is the spread plate technique set up? -ANSWER✅✅✅✅The sample is poured
onto a solid medium and spread using a L spreader
What cell counting method is used for counting colonies from drinking water? -
ANSWER✅✅✅✅Membrane filtration
AND VERIFIED CORRECT SOLUTIONS ||
100% GUARANTEED PASS BRAND NEW
VERSION
4 Basic Steps of binary fission -ANSWER✅✅✅✅1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly -ANSWER✅✅✅✅FtsZ assembles Z ring to form divisome
Generation Time -ANSWER✅✅✅✅time it takes to double the population
Lag Phase -ANSWER✅✅✅✅Cells adjust to culture medium; no change in population
What factors determine the duration of the lag phase? -ANSWER✅✅✅✅Genetic
makeup, media composition, initial inoculum size
During which phase of the growth curve is it most appropriate for the use of antibiotics?
-ANSWER✅✅✅✅Log phase
Following the death phase of the growth curve, ____ cells are considered the surviving
cells & are considered clinically important due to their association with chronic
infections, such as Tuberculosis. -ANSWER✅✅✅✅Persisters
Log Phase -ANSWER✅✅✅✅Binary fission occurs; cell replication > cell death
Stationary Phase -ANSWER✅✅✅✅Resources become depleted; cell respiration=cell
death
Death Phase -ANSWER✅✅✅✅Endospores can form; cell replication < cell death
Close System Cultures -ANSWER✅✅✅✅Have finite resources
Open System Cultures -ANSWER✅✅✅✅-Nutrients and air are replenished
-Dead cells and waste are removed
, -Beneficial for industrial microbiology
Microscopic Cell Count -ANSWER✅✅✅✅Cells are counted under a microscope using
a laser etched slide
In using the direct microscopic cell count method, you will be able to distinguish
between live vs dead cells. -ANSWER✅✅✅✅False
Fluorescence Staining -ANSWER✅✅✅✅Cells are counted under a microscope or flow
cytometer
In fluorescent staining, the end product results in live cells staining ___________? -
ANSWER✅✅✅✅Green
In fluorescent staining, the end product results in dead cells staining ___________? -
ANSWER✅✅✅✅Red
Coulter counting -ANSWER✅✅✅✅Detects electrical resistance change due to cell
density
Viable Cell Counts -ANSWER✅✅✅✅Samples are diluted and grown on solid media
What type of cells are counted when using the viable plate count method? -
ANSWER✅✅✅✅Living
Countable Range -ANSWER✅✅✅✅30-300 CFU/ml
Optical Density -ANSWER✅✅✅✅-Measured with spectrophotometer
-Light is passed thru culture and measured on other side
Which method of measuring growth is considered an indirect approach? -
ANSWER✅✅✅✅Optical density
Which method of measuring growth can be used on living or dead cells? -
ANSWER✅✅✅✅Fluorescent stain
How is the pour plate technique set up? -ANSWER✅✅✅✅The sample is mixed with a
warm agar ranging from 45-50 C, poured onto sterile plated and swirled to mix
How is the spread plate technique set up? -ANSWER✅✅✅✅The sample is poured
onto a solid medium and spread using a L spreader
What cell counting method is used for counting colonies from drinking water? -
ANSWER✅✅✅✅Membrane filtration
