CPIA Exam Study Guide | Newest Questions
and Answers | 2026 Update | Expert Verified
• The Health Research Extension Act of 1985 directs ______ to establish
guidelines for the proper care and treatment of animals used in research -✓✓ the
Secretary of Health and Human Services
• In 1986, the PHS Policy incorporated the ________ by the Interagency Research
Animal Committee and adopted by US Government agencies that use vertebrate
animals -✓✓ U.S. Government Principles for the Utilization and Care of
Vertebrate Animals Used in Testing, Research, and Training
• IACUC membership requirements for PHS Assurance -✓✓ 5 members:
One veterinarian (training/experience in lab animal medicine)
One scientist (experience in research)
One nonscientific member
One not affiliated member
• PHS Policy mandates that animal care and use programs must operate in
accordance with four specific laws and guidelines -✓✓ 1. The Animal Welfare Act
2. United States Government Principles for the Utilization and Care of Vertebrate
Animals Used in Testing, Research, and Training
3. The Guide for the Care and Use of Laboratory Animals
4. AVMA Guidelines for the Euthanasia of Animals
• Code of Federal Regulations, Title 9, Chapter 1, Subchapter A, Parts 1, 2, and 3 -
✓✓ Animal Welfare Regulations that are implemented by the USDA/APHIS/AC
,• Clarified humane care, minimization of pain and distress, consideration of
alternatives, institutional animal care and use committees, psychological well-
being of primates, and exercise for dogs -✓✓ 1985 amendments to the AWA
• Which term is defined in the Guide as "all measures to control known or
unknown infections in laboratory animals."
a. Biosecurity
b. Stabilization
c. Quarantine
d. Biosafety -✓✓ a. Biosecurity
• Which of the following statements about multiple survival surgical procedures
on a single animal is TRUE?
a. The Guide's definition of "major" surgery is rigid, and does not provide for any
interpretation by the veterinarian or the IACUC.
b. When USDA-covered species are used, the Animal Welfare Regulations'
definition of "major" surgery takes precedence.
c. The Animal Welfare Regulations allow for the interpretation of "major" surgery
to be evaluated on a case-by-case basis by the IACUC.
d. The Guide's requirement that multiple major survival surgeries must be
scientifically justified and included in the IACUC-approved protocol only applies to
non-rodent mammals. -✓✓ b. When USDA-covered species are used, the Animal
Welfare Regulations' definition of "major" surgery takes precedence.
• Animals using which type of thermoregulation depend mostly on environmental
temperature to maintain their internal physiology? -✓✓ Poikilothermic
, • Which should be provided in the environment of a semi-aquatic animal?
a. porous substrate
b. recirculating system
c. a terrestrial area
d. low humidity level -✓✓ c. a terrestrial area
• Which statement is TRUE for the use of agricultural animals in biomedical
research, when they are maintained in a farm setting?
a. These animals are covered by the Guide but not by the Animal Welfare Act.
b. These animals are only covered by the Ag Guide.
c. These animals are covered by the Animal Welfare Act but not by the Guide.
d. These animals are covered by the Guide but will benefit from the principles and
practices detailed in the Ag Guide. -✓✓ d. These animals are covered by the Guide
but will benefit from the principles and practices detailed in the Ag Guide.
• What is the minimum space required for a female mouse and her litter?
a. 75 in2
b. 51 in2
c. 330 in2
d. 124 in2 -✓✓ b. 51 in2
• Which are used to standardize the method for performing specific institutional
practices and procedures?
a. Policies
and Answers | 2026 Update | Expert Verified
• The Health Research Extension Act of 1985 directs ______ to establish
guidelines for the proper care and treatment of animals used in research -✓✓ the
Secretary of Health and Human Services
• In 1986, the PHS Policy incorporated the ________ by the Interagency Research
Animal Committee and adopted by US Government agencies that use vertebrate
animals -✓✓ U.S. Government Principles for the Utilization and Care of
Vertebrate Animals Used in Testing, Research, and Training
• IACUC membership requirements for PHS Assurance -✓✓ 5 members:
One veterinarian (training/experience in lab animal medicine)
One scientist (experience in research)
One nonscientific member
One not affiliated member
• PHS Policy mandates that animal care and use programs must operate in
accordance with four specific laws and guidelines -✓✓ 1. The Animal Welfare Act
2. United States Government Principles for the Utilization and Care of Vertebrate
Animals Used in Testing, Research, and Training
3. The Guide for the Care and Use of Laboratory Animals
4. AVMA Guidelines for the Euthanasia of Animals
• Code of Federal Regulations, Title 9, Chapter 1, Subchapter A, Parts 1, 2, and 3 -
✓✓ Animal Welfare Regulations that are implemented by the USDA/APHIS/AC
,• Clarified humane care, minimization of pain and distress, consideration of
alternatives, institutional animal care and use committees, psychological well-
being of primates, and exercise for dogs -✓✓ 1985 amendments to the AWA
• Which term is defined in the Guide as "all measures to control known or
unknown infections in laboratory animals."
a. Biosecurity
b. Stabilization
c. Quarantine
d. Biosafety -✓✓ a. Biosecurity
• Which of the following statements about multiple survival surgical procedures
on a single animal is TRUE?
a. The Guide's definition of "major" surgery is rigid, and does not provide for any
interpretation by the veterinarian or the IACUC.
b. When USDA-covered species are used, the Animal Welfare Regulations'
definition of "major" surgery takes precedence.
c. The Animal Welfare Regulations allow for the interpretation of "major" surgery
to be evaluated on a case-by-case basis by the IACUC.
d. The Guide's requirement that multiple major survival surgeries must be
scientifically justified and included in the IACUC-approved protocol only applies to
non-rodent mammals. -✓✓ b. When USDA-covered species are used, the Animal
Welfare Regulations' definition of "major" surgery takes precedence.
• Animals using which type of thermoregulation depend mostly on environmental
temperature to maintain their internal physiology? -✓✓ Poikilothermic
, • Which should be provided in the environment of a semi-aquatic animal?
a. porous substrate
b. recirculating system
c. a terrestrial area
d. low humidity level -✓✓ c. a terrestrial area
• Which statement is TRUE for the use of agricultural animals in biomedical
research, when they are maintained in a farm setting?
a. These animals are covered by the Guide but not by the Animal Welfare Act.
b. These animals are only covered by the Ag Guide.
c. These animals are covered by the Animal Welfare Act but not by the Guide.
d. These animals are covered by the Guide but will benefit from the principles and
practices detailed in the Ag Guide. -✓✓ d. These animals are covered by the Guide
but will benefit from the principles and practices detailed in the Ag Guide.
• What is the minimum space required for a female mouse and her litter?
a. 75 in2
b. 51 in2
c. 330 in2
d. 124 in2 -✓✓ b. 51 in2
• Which are used to standardize the method for performing specific institutional
practices and procedures?
a. Policies
