AAB MT BASIC KNOWLEDGE UPDATED
COMPREHENSIVE QUESTIONS AND ANSWERS SURE
A+
✔✔Semi-automated micropipettes - ✔✔contain or deliver small volumes of liquid
ranging from 1 to 1000ul
✔✔2 types of displacement for semi-automated pipettes - ✔✔air displacement: forward
& reverse
positive displacement
✔✔Types of glass pipettes - ✔✔-transfer/volumetric
-measuring/graduated
-to contain (TC) or rinse-out pipettes
-to deliver (TD) pipettes
-to deliver/blow-out
✔✔Transfer/volumetric pipettes - ✔✔have a bulb in the middle of the pipette with a
tapered delivery tip
✔✔Measuring/Graduated pipette - ✔✔uniform in diameter with a tapered delivery tip
, ✔✔To Contain (TC) or Rinse-out pipettes - ✔✔must be rinsed with the appropriate
solvent after the initial liquid has been drained from these pipettes
✔✔To Deliver (TD) pipettes - ✔✔filled & allowed to drain by gravity
-must be vertically and the tip placed against the side of the receiving vessel
✔✔To Deliver/Blow Out pipette - ✔✔are filled and allowed to drain, after which the
remaining fluid in the tip is blown out.
-easily identified by the 2 frosted bands near mouthpiece of the pipette
✔✔Temperature of Heat Blocks/Water baths - ✔✔36C-38C
✔✔Temperature of Refrigerators - ✔✔Laboratory 2C-8C
Blood Banking 1C-6C
✔✔Temperature of Freezers - ✔✔Laboratory <18C
Blood Bank <65C
✔✔Bright-field microscopy - ✔✔-simplest of all the light microscopy
-sample illumination is via transmitted white light illuminated from below and observed
from above
-most commonly used microscope
✔✔Dark-field microscopy - ✔✔-technique for improving the contrast of unstained,
transparent specimen
-uses a carefully aligned light source to minimize the quantity of directly transmitted light
entering the image plane, collecting only the light scattered by sample
✔✔Electron microscopy - ✔✔-electrons illuminate a specimen and create an enlarged
image
-uses electrostatic and electromagnetic lenses in forming the image by controlling the
electron beam to focus it at a specific plane relative to the specimen in a manner similar
to how a light microscope uses glass lenses to focus light on or through a specimen to
form an image
✔✔Fluorescent microscopy - ✔✔-uses fluorescence instead of reflection and absorption
to view object
✔✔Phase-contrast microscopy - ✔✔-widely used technique in which small phase shifts
in the light passing through a transparent specimen show differences in refractive index
as difference in contrast
✔✔Polarized microscopy - ✔✔-technique only produces transmitted polarized light
COMPREHENSIVE QUESTIONS AND ANSWERS SURE
A+
✔✔Semi-automated micropipettes - ✔✔contain or deliver small volumes of liquid
ranging from 1 to 1000ul
✔✔2 types of displacement for semi-automated pipettes - ✔✔air displacement: forward
& reverse
positive displacement
✔✔Types of glass pipettes - ✔✔-transfer/volumetric
-measuring/graduated
-to contain (TC) or rinse-out pipettes
-to deliver (TD) pipettes
-to deliver/blow-out
✔✔Transfer/volumetric pipettes - ✔✔have a bulb in the middle of the pipette with a
tapered delivery tip
✔✔Measuring/Graduated pipette - ✔✔uniform in diameter with a tapered delivery tip
, ✔✔To Contain (TC) or Rinse-out pipettes - ✔✔must be rinsed with the appropriate
solvent after the initial liquid has been drained from these pipettes
✔✔To Deliver (TD) pipettes - ✔✔filled & allowed to drain by gravity
-must be vertically and the tip placed against the side of the receiving vessel
✔✔To Deliver/Blow Out pipette - ✔✔are filled and allowed to drain, after which the
remaining fluid in the tip is blown out.
-easily identified by the 2 frosted bands near mouthpiece of the pipette
✔✔Temperature of Heat Blocks/Water baths - ✔✔36C-38C
✔✔Temperature of Refrigerators - ✔✔Laboratory 2C-8C
Blood Banking 1C-6C
✔✔Temperature of Freezers - ✔✔Laboratory <18C
Blood Bank <65C
✔✔Bright-field microscopy - ✔✔-simplest of all the light microscopy
-sample illumination is via transmitted white light illuminated from below and observed
from above
-most commonly used microscope
✔✔Dark-field microscopy - ✔✔-technique for improving the contrast of unstained,
transparent specimen
-uses a carefully aligned light source to minimize the quantity of directly transmitted light
entering the image plane, collecting only the light scattered by sample
✔✔Electron microscopy - ✔✔-electrons illuminate a specimen and create an enlarged
image
-uses electrostatic and electromagnetic lenses in forming the image by controlling the
electron beam to focus it at a specific plane relative to the specimen in a manner similar
to how a light microscope uses glass lenses to focus light on or through a specimen to
form an image
✔✔Fluorescent microscopy - ✔✔-uses fluorescence instead of reflection and absorption
to view object
✔✔Phase-contrast microscopy - ✔✔-widely used technique in which small phase shifts
in the light passing through a transparent specimen show differences in refractive index
as difference in contrast
✔✔Polarized microscopy - ✔✔-technique only produces transmitted polarized light
