BIO 110 Exam 3 - DNA Tools &
Biotechnology Questions With Correct
Answers
gene cloning - CORRECT ANSWER✔✔-one method to amplify (copy) a
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specific gene |
Plasmid - CORRECT ANSWER✔✔-A small, circular section of extra DNA
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that confers one or more traits to a bacterium and can be reproduced
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separately from the main bacterial genetic code.
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Gene cloning steps - CORRECT ANSWER✔✔-1. Gene inserted into
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plasmid
2. Plasmid put into bacterial cell (E. Coli)
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3. Host cell grown in culture to form a clone of cells containing the
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cloned gene of interest
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4. The proteins are harvested and used for research
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E. coli - CORRECT ANSWER✔✔-a bacterium commonly found in the
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intestines of humans which is used to make copies of genes.
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, Recombinant DNA - CORRECT ANSWER✔✔-DNA produced by | | | | | | |
combining DNA from different sources | | | |
cloning vector - CORRECT ANSWER✔✔-A DNA molecule known to
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replicate autonomously in a host cell, to which a segment of DNA may
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be spliced to allow its replication; for example, a plasmid or an artificial
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chromosome.
How is insulin made? - CORRECT ANSWER✔✔-Cultures of this insulin
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producing bacteria containing the cloned DNA can be grown. The
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bacteria is killed and the insulin is collected and purified. Now you have
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human insulin. |
restriction enzymes - CORRECT ANSWER✔✔-Enzyme that cuts DNA at a
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specific sequence of nucleotides
| | |
"sticky ends" - CORRECT ANSWER✔✔-Single stranded ends of DNA left
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after cutting with enzymes
| | |
DNA ligase - CORRECT ANSWER✔✔-enzyme that chemically links DNA
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fragments together |
Any dsDNA cut with the same restriction enzyme has the same
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complementary ___ - CORRECT ANSWER✔✔-sticky ends | | | | |
Biotechnology Questions With Correct
Answers
gene cloning - CORRECT ANSWER✔✔-one method to amplify (copy) a
| | | | | | | | | |
specific gene |
Plasmid - CORRECT ANSWER✔✔-A small, circular section of extra DNA
| | | | | | | | | |
that confers one or more traits to a bacterium and can be reproduced
| | | | | | | | | | | | |
separately from the main bacterial genetic code.
| | | | | |
Gene cloning steps - CORRECT ANSWER✔✔-1. Gene inserted into
| | | | | | | | |
plasmid
2. Plasmid put into bacterial cell (E. Coli)
| | | | | | |
3. Host cell grown in culture to form a clone of cells containing the
| | | | | | | | | | | | | |
cloned gene of interest
| | |
4. The proteins are harvested and used for research
| | | | | | | |
E. coli - CORRECT ANSWER✔✔-a bacterium commonly found in the
| | | | | | | | | |
intestines of humans which is used to make copies of genes.
| | | | | | | | | |
, Recombinant DNA - CORRECT ANSWER✔✔-DNA produced by | | | | | | |
combining DNA from different sources | | | |
cloning vector - CORRECT ANSWER✔✔-A DNA molecule known to
| | | | | | | | |
replicate autonomously in a host cell, to which a segment of DNA may
| | | | | | | | | | | | |
be spliced to allow its replication; for example, a plasmid or an artificial
| | | | | | | | | | | | |
chromosome.
How is insulin made? - CORRECT ANSWER✔✔-Cultures of this insulin
| | | | | | | | | |
producing bacteria containing the cloned DNA can be grown. The
| | | | | | | | | |
bacteria is killed and the insulin is collected and purified. Now you have
| | | | | | | | | | | | |
human insulin. |
restriction enzymes - CORRECT ANSWER✔✔-Enzyme that cuts DNA at a
| | | | | | | | | |
specific sequence of nucleotides
| | |
"sticky ends" - CORRECT ANSWER✔✔-Single stranded ends of DNA left
| | | | | | | | | |
after cutting with enzymes
| | |
DNA ligase - CORRECT ANSWER✔✔-enzyme that chemically links DNA
| | | | | | | | |
fragments together |
Any dsDNA cut with the same restriction enzyme has the same
| | | | | | | | | | |
complementary ___ - CORRECT ANSWER✔✔-sticky ends | | | | |
