Bio Lab Exam II Questions and Answers with
Verified Solutions | Latest Updated 2026
Why do the cheek cells need to be Lysing breaks open the cells so the DNA is
lysed before setting up PCR? accessible.
What does PCR stand for? Polymerase chain reaction
Which of the following will be Master mix
components in your PCR mixture? Human DNA
What are you trying to amplify Short tandem repeats from specific genes
using
PCR in this experiment?
,Match the definitions with the PCR Denaturation: The DNA template is heated
terminology. so the
Denaturation strands separate.
Annealing Annealing: The reaction is cooled so the
Extension primers
Thermocycler can stick to the DNA template.
Extension: The temperature is changed to
the ideal
temperature for Taq polymerase to build
(synthesize) a new strand based on the
template.
Thermocycler:
The machine that controls the temperature
of the
reaction and has been programmed to
move
through the heating and cooling steps.
When you arrive at lab, you need When you arrive at lab, you need to be
to wearing
be wearing [a] shoes. When it is close-toed shoes. When it is time to start
time experiments, you must wear goggles and,
to start experiments, you must to keep
wear you safe, they must remain over your eyes
[b] and, to keep you safe, they while
must anyone in the lab is doing experiments.
remain over your [c] while [d] in
the
lab is doing experiments.
, Match the function to the name of Centrifuge: Spins at a high speed to
the lab equipment. separate
Centrifuge things by density (denser stuff moves
Thermocycler lower in the
Micropipette tube).
Chelex beads Thermocycler: Can be programmed to
keep
samples/reactions at specific temperatures
for
specific lengths of time.
Micropipette: Measures and transfers very
specific,
usually small, volumes of fluid accurately.
Chelex beads: Bind metal ions so they can
be
removed from the sample.
[x] uses a/an [y] called Taq [PCR] uses a/an [enzyme] called Taq
polymerase to create [z] of copies polymerase
of to create [thousands] of copies of a
a specific region of [w]. specific region
of [DNA].
Verified Solutions | Latest Updated 2026
Why do the cheek cells need to be Lysing breaks open the cells so the DNA is
lysed before setting up PCR? accessible.
What does PCR stand for? Polymerase chain reaction
Which of the following will be Master mix
components in your PCR mixture? Human DNA
What are you trying to amplify Short tandem repeats from specific genes
using
PCR in this experiment?
,Match the definitions with the PCR Denaturation: The DNA template is heated
terminology. so the
Denaturation strands separate.
Annealing Annealing: The reaction is cooled so the
Extension primers
Thermocycler can stick to the DNA template.
Extension: The temperature is changed to
the ideal
temperature for Taq polymerase to build
(synthesize) a new strand based on the
template.
Thermocycler:
The machine that controls the temperature
of the
reaction and has been programmed to
move
through the heating and cooling steps.
When you arrive at lab, you need When you arrive at lab, you need to be
to wearing
be wearing [a] shoes. When it is close-toed shoes. When it is time to start
time experiments, you must wear goggles and,
to start experiments, you must to keep
wear you safe, they must remain over your eyes
[b] and, to keep you safe, they while
must anyone in the lab is doing experiments.
remain over your [c] while [d] in
the
lab is doing experiments.
, Match the function to the name of Centrifuge: Spins at a high speed to
the lab equipment. separate
Centrifuge things by density (denser stuff moves
Thermocycler lower in the
Micropipette tube).
Chelex beads Thermocycler: Can be programmed to
keep
samples/reactions at specific temperatures
for
specific lengths of time.
Micropipette: Measures and transfers very
specific,
usually small, volumes of fluid accurately.
Chelex beads: Bind metal ions so they can
be
removed from the sample.
[x] uses a/an [y] called Taq [PCR] uses a/an [enzyme] called Taq
polymerase to create [z] of copies polymerase
of to create [thousands] of copies of a
a specific region of [w]. specific region
of [DNA].
