MMSC 407 exam 1 UPDATED ACTUAL and Correct Answers

MMSC 407 exam 1 UPDATED ACTUAL and Correct Answers
Question: light
Answer:
energy that propagates and transfers as waves

Question: Wavelength
Answer:
Distance between wave peaks (nm)

Question: Frequency
Answer:
Number of waves per second

Question: UV-Visible region
Answer:
200-700 nm
Used for quantitative analysis

Question: visible spectrum
Answer:
detectable by the human eye
•Wavelength range: 380-750 nm

Question: Energy
Answer:
inversely related to wavelength
(shorter wavelength = higher energy)

Question: Fluorescence
Answer:
Highly sensitive**
-Measures emitted light after excitation
-used in immunoassays, molecular diagnostics, flow cytometry

Question: Transmittance (T)
Answer:

, Percentage of light that passes through a sample
Higher analyte concentration ’ lower transmittance

Question: transmittance formula
Answer:
T = I/IO
I = transmitted light (output)
Io = original light intensity

Question: Absorbance (A)
Answer:
Directly proportional to analyte concentration**

Question: absorbance and transmittance formula
Answer:
A=-logT
A=log(1/T)
A =2 - log(%T)

Question: Spectrophotometry
Answer:
-measures the amount of light absorbed by a substance (chromogen) at a specific
wavelength to determine analyte concentration

Question: Beer-Lamber Law
Answer:
A=εbc
A: absorbance
ε: molar absorptivity
b: path length of cuvette
c: concentration

Question: t/f
Answer:
Absorbance is directly proportional to analyte concentration: true

Question: Deuterium lamp
Answer: