ASCP: Board of Certification MLT
Computer Adaptive Practice Test
Questions Fully Solved 2026-2027
Updated.
Patients with antibody to the following antigen are immune to Hepatitis B:
A: Core Antigen
B: Surface antigen
C: e antigen
D: Delta antigen
**This is a Level 4 Question** - Answer B
Which of the following organisms is BEST VISUALIZED by use of a darkfield microscope?
A: Poxviridae
B: Entamoeba
C: Borrelia
D: Streptococcus
**This is a Level 5 Question** - Answer C
What is the MOST LIKELY cause of the ABO discrepancy when the following results were
obtained from a first-time 29-year-old, blood donor?
Forward: Anti-A = Negative; Anti-B = Negative
Reverse: A1 cells = Negative; B cells = 3+
A: Loss of antigen due to disease
B: Acquired B
C: Low immunoglobulin levels
D: Weak subgroup of A
**This is a Level 4 Question** - Answer D
,An aerobic Gram-Negative coccobacillus was isolated from a nasopharyngeal swab 48 hours
after culture from a 6-month-old with the following culture characteristics on Bordet-Gengou
agar:
Small zone of Beta-hemolysis
Urease: Positive (24hrs)
Oxidase: Negative
Motility: Negative
The MOST probable ID of this isolate is:
A: Pasteurella multocida
B: Bordetella pertussis
C: Bordetella parapertussis
D: Bordetella bronchiseptica
**This is a Level 8 Question** - Answer C
Disorders in which coarse basophilic stippling can be seen are in all of the following except?
A: Polycythemia
B: Thalassemia
C: Disorders of abnormal heme synthesis
D: Lead poisoning
**This is a Level 5 Question** - Answer A
Which of the following statements concerning Tamm-Horsfall protein is FALSE?
A: It is the protein that is predominantly detected by a urine reagent strip test
B: Hyaline casts are formed by this protein in the distal tubule
C: It acts as a coating & lubricant in the tubules
D: It is produced by the tubules
, **This is a Level 4 Question** - Answer A
All the following are reasons for conducting compatibility testing EXCEPT:
A: Prevent recipient alloimmunization
B: Verify ABO & Rh
C: Select proper blood products
D: Detect antibodies against donor cells
**This is a Level 6 Question** - Answer A
The historical medium of choice for isolation of Francisella is:
A: Chocolate agar
B: Cysteine blood glucose agar
C: Modified Thayer-Martin agar
D: MacConkey agar
**This is a Level 4 Question** - Answer B
To screen for UTIs, leukocyte esterase results should be evaluated along with the results from
which of these other reagent strip tests?
A: Urobilinogen
B: Bilirubin
C: Nitrite
D: Glucose
**This is a Level 2 Question** - Answer C
The process of freeze-drying of bacteria is called:
A: Agglutination
B: Inhibition
C: Lyophilization
Computer Adaptive Practice Test
Questions Fully Solved 2026-2027
Updated.
Patients with antibody to the following antigen are immune to Hepatitis B:
A: Core Antigen
B: Surface antigen
C: e antigen
D: Delta antigen
**This is a Level 4 Question** - Answer B
Which of the following organisms is BEST VISUALIZED by use of a darkfield microscope?
A: Poxviridae
B: Entamoeba
C: Borrelia
D: Streptococcus
**This is a Level 5 Question** - Answer C
What is the MOST LIKELY cause of the ABO discrepancy when the following results were
obtained from a first-time 29-year-old, blood donor?
Forward: Anti-A = Negative; Anti-B = Negative
Reverse: A1 cells = Negative; B cells = 3+
A: Loss of antigen due to disease
B: Acquired B
C: Low immunoglobulin levels
D: Weak subgroup of A
**This is a Level 4 Question** - Answer D
,An aerobic Gram-Negative coccobacillus was isolated from a nasopharyngeal swab 48 hours
after culture from a 6-month-old with the following culture characteristics on Bordet-Gengou
agar:
Small zone of Beta-hemolysis
Urease: Positive (24hrs)
Oxidase: Negative
Motility: Negative
The MOST probable ID of this isolate is:
A: Pasteurella multocida
B: Bordetella pertussis
C: Bordetella parapertussis
D: Bordetella bronchiseptica
**This is a Level 8 Question** - Answer C
Disorders in which coarse basophilic stippling can be seen are in all of the following except?
A: Polycythemia
B: Thalassemia
C: Disorders of abnormal heme synthesis
D: Lead poisoning
**This is a Level 5 Question** - Answer A
Which of the following statements concerning Tamm-Horsfall protein is FALSE?
A: It is the protein that is predominantly detected by a urine reagent strip test
B: Hyaline casts are formed by this protein in the distal tubule
C: It acts as a coating & lubricant in the tubules
D: It is produced by the tubules
, **This is a Level 4 Question** - Answer A
All the following are reasons for conducting compatibility testing EXCEPT:
A: Prevent recipient alloimmunization
B: Verify ABO & Rh
C: Select proper blood products
D: Detect antibodies against donor cells
**This is a Level 6 Question** - Answer A
The historical medium of choice for isolation of Francisella is:
A: Chocolate agar
B: Cysteine blood glucose agar
C: Modified Thayer-Martin agar
D: MacConkey agar
**This is a Level 4 Question** - Answer B
To screen for UTIs, leukocyte esterase results should be evaluated along with the results from
which of these other reagent strip tests?
A: Urobilinogen
B: Bilirubin
C: Nitrite
D: Glucose
**This is a Level 2 Question** - Answer C
The process of freeze-drying of bacteria is called:
A: Agglutination
B: Inhibition
C: Lyophilization
