ASCP SCYM STUDY QUESTIONS WITH CORRECT ANSWERS
2026
Sample Processing - CORRECT ANSWERS -Optimized antibody concentration
Defined range of cell #
Define sample handling, time and temperature
Define acceptable time of delay for acquisition
CD2 - CORRECT ANSWERS -T cells: NK cells
CD3 - CORRECT ANSWERS -T cells
CD4 - CORRECT ANSWERS -T helper cells, monocytes (dimly expressed)
CD5 - CORRECT ANSWERS -T cells; Some B Cells
CD8 - CORRECT ANSWERS -T cytotoxic cells, macrophages
CD10 - CORRECT ANSWERS -Immature T, B Cells, PMN's
CD13 - CORRECT ANSWERS -Monocytes, PMN's
CD14 - CORRECT ANSWERS -Monocytes, PMN's (dimly expressed)
CD16 - CORRECT ANSWERS -NK cells, PMN's, monocytes
,CD19 - CORRECT ANSWERS -B Cells
CD20 - CORRECT ANSWERS -B Cells
CD25 - CORRECT ANSWERS -Activated T Cells
CD33 - CORRECT ANSWERS -Monocytes
CD34 - CORRECT ANSWERS -Stem/Progenitor Cells
CD45 - CORRECT ANSWERS -Leukocytes
CD56 - CORRECT ANSWERS -NK cells, monocytes (dimly expressed)
Anti-Kappa - CORRECT ANSWERS -B Cells
Anti-Lambda - CORRECT ANSWERS -B Cells
Tdt - CORRECT ANSWERS -Immature Lymphocytes
Monoclonal Antibody Staining Direct Staining - CORRECT ANSWERS -Rapid single step staining
Can use multiple antibodies from the same host
Monoclonal Antibody Staining Indirect Staining - CORRECT ANSWERS -Secondary amplifies
single
, A few labeled secondaries can detect many primaries
Clinical Applications of Flow - CORRECT ANSWERS --Instrument Performance
-Integrity of the Specimen
-Quality in Processing of Specimens
-Appropriate Reagents and Controls
-Qualified Technical Staff
-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist to ensure the accurate
reporting of flow cytometric results.
Lymphocytes Subset Enumeration (Immunophenotyping) - CORRECT ANSWERS -2 Tubes: 1-
CD3/CD4/CD8/CD45
2- CD3/CD19/CD16&CD56/CD45
-May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.
-Results reported as proportion (%) of total lymphocyte population.
CD4 Counts in HIV Infection - CORRECT ANSWERS --Asses degree of immunodeficiency
-Diagnose of AIDS
-Institution of Anti-Retroviral (ARV) therapy and opportunistic infection (OI) prophylaxis
-Monitoring immune reconstitution
CD4 Immunophenotyping Panels - CORRECT ANSWERS -
2 color - CORRECT ANSWERS -Isotype Ctrl
CD45/CD14
CD3/CD4
CD3/CD8
2026
Sample Processing - CORRECT ANSWERS -Optimized antibody concentration
Defined range of cell #
Define sample handling, time and temperature
Define acceptable time of delay for acquisition
CD2 - CORRECT ANSWERS -T cells: NK cells
CD3 - CORRECT ANSWERS -T cells
CD4 - CORRECT ANSWERS -T helper cells, monocytes (dimly expressed)
CD5 - CORRECT ANSWERS -T cells; Some B Cells
CD8 - CORRECT ANSWERS -T cytotoxic cells, macrophages
CD10 - CORRECT ANSWERS -Immature T, B Cells, PMN's
CD13 - CORRECT ANSWERS -Monocytes, PMN's
CD14 - CORRECT ANSWERS -Monocytes, PMN's (dimly expressed)
CD16 - CORRECT ANSWERS -NK cells, PMN's, monocytes
,CD19 - CORRECT ANSWERS -B Cells
CD20 - CORRECT ANSWERS -B Cells
CD25 - CORRECT ANSWERS -Activated T Cells
CD33 - CORRECT ANSWERS -Monocytes
CD34 - CORRECT ANSWERS -Stem/Progenitor Cells
CD45 - CORRECT ANSWERS -Leukocytes
CD56 - CORRECT ANSWERS -NK cells, monocytes (dimly expressed)
Anti-Kappa - CORRECT ANSWERS -B Cells
Anti-Lambda - CORRECT ANSWERS -B Cells
Tdt - CORRECT ANSWERS -Immature Lymphocytes
Monoclonal Antibody Staining Direct Staining - CORRECT ANSWERS -Rapid single step staining
Can use multiple antibodies from the same host
Monoclonal Antibody Staining Indirect Staining - CORRECT ANSWERS -Secondary amplifies
single
, A few labeled secondaries can detect many primaries
Clinical Applications of Flow - CORRECT ANSWERS --Instrument Performance
-Integrity of the Specimen
-Quality in Processing of Specimens
-Appropriate Reagents and Controls
-Qualified Technical Staff
-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist to ensure the accurate
reporting of flow cytometric results.
Lymphocytes Subset Enumeration (Immunophenotyping) - CORRECT ANSWERS -2 Tubes: 1-
CD3/CD4/CD8/CD45
2- CD3/CD19/CD16&CD56/CD45
-May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.
-Results reported as proportion (%) of total lymphocyte population.
CD4 Counts in HIV Infection - CORRECT ANSWERS --Asses degree of immunodeficiency
-Diagnose of AIDS
-Institution of Anti-Retroviral (ARV) therapy and opportunistic infection (OI) prophylaxis
-Monitoring immune reconstitution
CD4 Immunophenotyping Panels - CORRECT ANSWERS -
2 color - CORRECT ANSWERS -Isotype Ctrl
CD45/CD14
CD3/CD4
CD3/CD8
