MLS 4116: Clinical Bacteriology I,
Final Exam Review Questions with
Actual Answers.
Which types of bacteria are susceptible to penicillin? - Answer Gram +
Which types of bacteria are resistant to penicillin? - Answer Gram -
Which bacteria have mycolic acids in the cell wall? - Answer Mycobacteria
Norcardia
Which bacteria has sterols in the cell membrane but has no murein or peptidoglycan? - Answer
Mycoplasma
Which bacteria is hard to stain and are obligate intracellular parasites? - Answer Rickettsia
Which bacteria are obligate intracellular parasites and cannot synthesize ATP? - Answer
Chlamydia
Which media is used to select for Bordatella species? - Answer Bordet-Gengou agar
Selective media.
Which media is used to select for Legionella? - Answer Buffered Charcoal Yeast Agar
Selective Media
Which media is nutritive and used to grow most bacteria and observe hemolysis? - Answer
Blood agar.
Beta, alpha, gamma hemolysis.
What media contains thiamin, niacin, and biotin and is used to grow Brucella? - Answer
Brucella Anaerobic Blood Plates
Nutritive
Vitamin K and Hemin
What media is used to grow Neisseria and Haemophilus species? - Answer Chocolate Agar
Nutritive
, Factor X and Factor V
Which media is used to grow Bacillus cereus? - Answer Egg Yolk Agar
Differential.
Detects enzyme activity.
Which selective and differential media is used to select for gram - organisms as well as
differentiate between those that ferment lactose? - Answer MacConkey Agar
Selective/Differential
Salts are the selective component and lactose is the differential component.
Which selective and differential media is used to select for gram + Staphylococci as well as
differentiate between those that ferment mannitol? - Answer Mannitol Salt Agar
Selective/Differential
Salts are the selective component and mannitol is the differential component.
Which media is used to grow Campylobacter? - Answer Campy Blood Agar
Selective
Antibiotics inhibit fecal flora.
Which media is used as the base for aerobic susceptibility testing done in a lab? - Answer
Mueller Hinton agar
Nutritive
What media inhibits DNA synthesis of gram - bacteria to select for gram + organisms? - Answer
PEA Agar
Selective
Which media is used to select for species of Neisseria and contains vancomycin, colistin,
nystatin, and trimethoprim? - Answer Thayer-Martin Agar
Selective
Vancomycin : inhibits gram +
Colistin : inhibits gram -
Nystain : inhibits yeast
Trimetoprim : prevents swarming of Proteus
Final Exam Review Questions with
Actual Answers.
Which types of bacteria are susceptible to penicillin? - Answer Gram +
Which types of bacteria are resistant to penicillin? - Answer Gram -
Which bacteria have mycolic acids in the cell wall? - Answer Mycobacteria
Norcardia
Which bacteria has sterols in the cell membrane but has no murein or peptidoglycan? - Answer
Mycoplasma
Which bacteria is hard to stain and are obligate intracellular parasites? - Answer Rickettsia
Which bacteria are obligate intracellular parasites and cannot synthesize ATP? - Answer
Chlamydia
Which media is used to select for Bordatella species? - Answer Bordet-Gengou agar
Selective media.
Which media is used to select for Legionella? - Answer Buffered Charcoal Yeast Agar
Selective Media
Which media is nutritive and used to grow most bacteria and observe hemolysis? - Answer
Blood agar.
Beta, alpha, gamma hemolysis.
What media contains thiamin, niacin, and biotin and is used to grow Brucella? - Answer
Brucella Anaerobic Blood Plates
Nutritive
Vitamin K and Hemin
What media is used to grow Neisseria and Haemophilus species? - Answer Chocolate Agar
Nutritive
, Factor X and Factor V
Which media is used to grow Bacillus cereus? - Answer Egg Yolk Agar
Differential.
Detects enzyme activity.
Which selective and differential media is used to select for gram - organisms as well as
differentiate between those that ferment lactose? - Answer MacConkey Agar
Selective/Differential
Salts are the selective component and lactose is the differential component.
Which selective and differential media is used to select for gram + Staphylococci as well as
differentiate between those that ferment mannitol? - Answer Mannitol Salt Agar
Selective/Differential
Salts are the selective component and mannitol is the differential component.
Which media is used to grow Campylobacter? - Answer Campy Blood Agar
Selective
Antibiotics inhibit fecal flora.
Which media is used as the base for aerobic susceptibility testing done in a lab? - Answer
Mueller Hinton agar
Nutritive
What media inhibits DNA synthesis of gram - bacteria to select for gram + organisms? - Answer
PEA Agar
Selective
Which media is used to select for species of Neisseria and contains vancomycin, colistin,
nystatin, and trimethoprim? - Answer Thayer-Martin Agar
Selective
Vancomycin : inhibits gram +
Colistin : inhibits gram -
Nystain : inhibits yeast
Trimetoprim : prevents swarming of Proteus
