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NHA Phlebotomy Practice Test Questions & Answers 2026 | Latest Update | Complete Exam Prep Guide

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This NHA Phlebotomy Practice Test Questions & Answers 2026 study guide is a comprehensive preparation resource designed for candidates preparing for the National Healthcareer Association (NHA) Certified Phlebotomy Technician (CPT) certification examination. The guide includes practice questions and answers covering essential phlebotomy topics such as venipuncture procedures, capillary blood collection, specimen handling and processing, infection prevention and control, patient identification, laboratory safety, quality assurance, anatomy and physiology, medical terminology, and professional responsibilities in healthcare environments. Designed to support effective exam preparation, this resource helps learners reinforce core phlebotomy concepts, strengthen technical knowledge, improve confidence, and enhance readiness for certification testing through focused review and practice-based learning. Ideal for phlebotomy students, healthcare trainees, medical assistants, and certification candidates seeking a structured review of key phlebotomy concepts.

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NHA Phlebotomy Practice Test Questions With
Accurate Answers | Latest Update 2026 | Exam Prep
1. If a phlebotomy technician is required to draw blood from a newborn
weighing 4 kg, what would be the maximum volume they could safely draw,
assuming the same 1% limit applies?

4 mL

2 mL

3 mL

5 mL

2. What is the recommended method for handling an ammonia specimen after
collection?

Store the specimen at room temperature

Refrigerate the specimen immediately

Shake the specimen tube vigorously

Place the specimen tube in an ice-water slurry

3. To prevent hospital-acquired infections, a phlebotomist should clean items
that have been contaminated by blood with a 1:10 dilution of what and water?

isopropyl alcohol

hydrogen peroxide

sodium hypochlorite

benzalkonium chloride

,4. Phleb is preparing to perform a VP on px who states: I faint at the sight of
blood. Which position is optimal for px safety

sitting upright in chair

standing against wall

leaning on a stool

lying in reclined position

5. Describe the rationale behind the order of removing personal protective
equipment (PPE) after blood specimen collection.

Gowns are removed first to avoid spreading pathogens to the
environment.

Gloves should be removed first to prevent contamination of the
hands before touching other surfaces.

Face shields are removed first to protect the eyes from exposure.

Masks are removed first to ensure breathing is not obstructed.

6. What is the recommended equipment for collecting blood from patients with
fragile veins?

Capillary puncture device

Standard needle and vacuum tube

Larger gauge needle

Syringe and butterfly assembly

7. Describe why the lavender tube is preferred for collecting dermal samples in
phlebotomy.

, The lavender tube is preferred because it is the only tube that can
collect serum.

The lavender tube is preferred because it is the least expensive
option.

The lavender tube is preferred for collecting dermal samples
because it contains EDTA, an anticoagulant that helps preserve
blood components for accurate testing.

The lavender tube is preferred for its larger volume capacity.

8. Why is it essential for a phlebotomy technician to verify the specimen
identification code on a laboratory requisition?

To ensure accurate tracking and identification of the specimen.

To identify the technician responsible for the procedure.

To confirm the patient's insurance coverage.

To verify the patient's age.

9. Which of the following procedural steps for a singer stick should the
phlebotomist do first?

confirm the correct identity of the patient

position the blade of the lancet across the lines of the fingertip

select the appropriate part of the third or fourth finger

cleanse the site with an antiseptic wipe

10. If a phlebotomy technician mistakenly collects a red tube before the
lavender tube for a dermal sample, what impact could this have on the test
results?

, It could lead to inaccurate test results due to the lack of
anticoagulant.

It would improve the accuracy of the test results.

It would only affect the volume of the sample collected.

It would have no impact on the test results.

11. Why is it important for a phlebotomy technician to protect Vitamin B6 from
light during transport?

Vitamin B6 is sensitive to light, which can degrade the sample and
affect test results.

Vitamin B6 is not affected by light, so it can be transported without
special precautions.

Vitamin B6 must be stored at room temperature to prevent
contamination.

Vitamin B6 needs to be kept warm to maintain its integrity.

12. What is the correct method for a phlebotomy technician to remove gloves
after a venipuncture procedure?

Pull both gloves off at once

Wash hands before removing gloves

Use a paper towel to remove gloves

Remove the first glove so that it turns out during removal

13. If a phlebotomist declines the Hepatitis B vaccine offered by their employer,
what implications could this have for their workplace safety and compliance
with OSHA regulations?

The employer will be exempt from providing any further vaccinations.

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