MLT ASCP PRACTICE PRACTICE
EXAMINATION 2026 QUESTIONS
WITH ANSWERS GRADED A+
◍ dosage.
Answer: The ____________ effect is the phenomenon of stronger antigen
expression with homozygous genotypes that heterozygous genotypes.
◍ Standard precautions apply to all of the following except:a. Bloodb.
Cerebrospinal fluidc. Semend. Concentrated acids.
Answer: d. Concentrated acids
◍ The most important practice in preventing the spread of disease is:a.
Wearing masks during patient contactb. Proper hand washingc. Wearing
disposable laboratory coatsd. Identifying specimens from known or
suspected HIV- and HBV-infected patients with a red label.
Answer: b. Proper hand washing
◍ The appropriate dilution of bleach to use in laboratory disinfection is:a.
1:2b. 1:5c. 1:10d. 1:100.
Answer: d. 1:10
◍ 37°C.
Answer: At which temperature do warm antibodies react with red blood
cells?
◍ How frequently should fire alarms and sprinkler systems be tested?a.
Weeklyb. Monthlyc. Quarterlyd. Annually.
Answer: c. Quarterly
◍ IgG1 and IgG3.
Answer: Which IgG subclasses are most efficient at activating complement?
,◍ Where should alcohol and other flammable chemicals be stored?a. In an
approved safety can or storage cabinet away from heat sourcesb. Under a
hood and arranged alphabetically for ease of identification in an
emergencyc. In a refrigerator at 2 C to 8 C to reduce volatilizationd. On a
low shelf in an area protected from light.
Answer: a. In an approved safety can or storage cabinet away from heat
sources
◍ The most frequent cause of needle punctures is:a. Patient movement during
venipunctureb. Improper disposal of phlebotomy equipmentc. Inattention
during removal of needle after venipunctured. Failure to attach needle firmly
to syringe or tube holder.
Answer: b. Improper disposal of phlebotomy equipment
◍ What class fire extinguisher would be appropriate to use on a fire in a
chemical cabinet?a. Class Ab. Class Bc. Class Cd. Class D.
Answer: b. Class B
◍ Select the needle most commonly used in standard venipuncture in an
adult:a. One inch, 18 gaugeb. One inch, 21 gaugec. One-half inch, 23
gauged. One-half inch, 25 gauge.
Answer: b. One inch, 21 gauge
◍ The process of formation and development of blood cells is termed:a.
Hematopoiesisb. Hematemesis c. Hematocytometryd. Hematorrhea.
Answer: a. Hematopoiesis
◍ During the second trimester of fetal development, the primary site of blood
cell production is:a. Bone marrowb. Spleenc. Lymph nodesd. Liver.
Answer: d. Liver
◍ Anti-P1.
Answer: Which of these antibodies is clinically insignificant?
◍ IgM.
Answer: Lewis antibodies are almost always which class?
,◍ IgG1.
Answer: Which IgG subclass is most abundant?
◍ Anti-H.
Answer: Which antibody is only found in people with the Bombay blood
group?
◍ Anti-HPA-1a.
Answer: Which maternal antibody is most commonly responsible for
causing fetal neonatal alloimmune thrombocytopenia (FNAIT)?
◍ normal saline.
Answer: Washed red blood cells are prepared by washing red cells with?
◍ Which of the following organs is responsible for the maturation of T
lymphocytes and regulation of their expression of CD4 and CD8?a. Spleenb.
Liverc. Thymusd. Bone marrow.
Answer: c. Thymus
◍ The best source of active bone marrow from a 20-year-old would be:a. Iliac
crestb. Femurc. Distal radius d. Tibia.
Answer: a. Iliac crest
◍ Physiologic programmed cell death is termed:a. Angiogenesisb. Apoptosisc.
Aneurysmd. Apohematics.
Answer: b. Apoptosis
◍ Albumin.
Answer: Which of these is a plasma derivative?
◍ to detect fetomaternal hemorrage in postpartum women.
Answer: What is the purpose of the rosette test?
◍ Which organ is the site of sequestration of platelets?a. Liver b. Thymusc.
Spleend. Bone marrow.
Answer: c. Spleen
◍ Which one of the following cells is a product of the CLP?a.
, Megakaryocyteb. T lymphocytec. Erythrocyted. Granulocyte.
Answer: b. T lymphocyte
◍ Rh-mother, Rh-fetus.
Answer: Which of these situations could result in hemolytic disease of the
newborn?
◍ Pink.
Answer: Which vacutainer tube is preferred for blood bank tests?
◍ What growth factor is produced in the kidneys and is used to treat anemia
associated with kidney disease?a. EPOb. TPOc. G-CSFd. KIT ligand.
Answer: a. EPO
◍ Which one of the following cytokines is required very early in the
differentiation of a hematopoietic stem cell?a. IL-2b. IL-8c. EPOd. FLT3
ligand.
Answer: d. FLT3 ligand
◍ 2.
Answer: Refer to the pedigree chart below:How many of the four children
would be expected to have type AB blood?
◍ Hematopoietic stem cells produce all lineages of blood cells in sufficient
quantities over the lifetime of an individual because they:a. Are unipotentb.
Have the ability of self-renewal by asymmetric divisionc. Are present in
large numbers in the bone marrow nichesd. Have a low mitotic potential in
response to growth factors.
Answer: b. Have the ability of self-renewal by asymmetric division
◍ Porcine.
Answer: A patient has hemophilia A and needs injections of factor VIII.
However, the patient's body has developed inhibitors to human factor VIII.
Which form of factor VIII could be given to this patient instead?
◍ by destroying fetal red blood cells in the mother's bloodstream.
Answer: How does anti-D immunoglobulin prevent Rhesus disease?
EXAMINATION 2026 QUESTIONS
WITH ANSWERS GRADED A+
◍ dosage.
Answer: The ____________ effect is the phenomenon of stronger antigen
expression with homozygous genotypes that heterozygous genotypes.
◍ Standard precautions apply to all of the following except:a. Bloodb.
Cerebrospinal fluidc. Semend. Concentrated acids.
Answer: d. Concentrated acids
◍ The most important practice in preventing the spread of disease is:a.
Wearing masks during patient contactb. Proper hand washingc. Wearing
disposable laboratory coatsd. Identifying specimens from known or
suspected HIV- and HBV-infected patients with a red label.
Answer: b. Proper hand washing
◍ The appropriate dilution of bleach to use in laboratory disinfection is:a.
1:2b. 1:5c. 1:10d. 1:100.
Answer: d. 1:10
◍ 37°C.
Answer: At which temperature do warm antibodies react with red blood
cells?
◍ How frequently should fire alarms and sprinkler systems be tested?a.
Weeklyb. Monthlyc. Quarterlyd. Annually.
Answer: c. Quarterly
◍ IgG1 and IgG3.
Answer: Which IgG subclasses are most efficient at activating complement?
,◍ Where should alcohol and other flammable chemicals be stored?a. In an
approved safety can or storage cabinet away from heat sourcesb. Under a
hood and arranged alphabetically for ease of identification in an
emergencyc. In a refrigerator at 2 C to 8 C to reduce volatilizationd. On a
low shelf in an area protected from light.
Answer: a. In an approved safety can or storage cabinet away from heat
sources
◍ The most frequent cause of needle punctures is:a. Patient movement during
venipunctureb. Improper disposal of phlebotomy equipmentc. Inattention
during removal of needle after venipunctured. Failure to attach needle firmly
to syringe or tube holder.
Answer: b. Improper disposal of phlebotomy equipment
◍ What class fire extinguisher would be appropriate to use on a fire in a
chemical cabinet?a. Class Ab. Class Bc. Class Cd. Class D.
Answer: b. Class B
◍ Select the needle most commonly used in standard venipuncture in an
adult:a. One inch, 18 gaugeb. One inch, 21 gaugec. One-half inch, 23
gauged. One-half inch, 25 gauge.
Answer: b. One inch, 21 gauge
◍ The process of formation and development of blood cells is termed:a.
Hematopoiesisb. Hematemesis c. Hematocytometryd. Hematorrhea.
Answer: a. Hematopoiesis
◍ During the second trimester of fetal development, the primary site of blood
cell production is:a. Bone marrowb. Spleenc. Lymph nodesd. Liver.
Answer: d. Liver
◍ Anti-P1.
Answer: Which of these antibodies is clinically insignificant?
◍ IgM.
Answer: Lewis antibodies are almost always which class?
,◍ IgG1.
Answer: Which IgG subclass is most abundant?
◍ Anti-H.
Answer: Which antibody is only found in people with the Bombay blood
group?
◍ Anti-HPA-1a.
Answer: Which maternal antibody is most commonly responsible for
causing fetal neonatal alloimmune thrombocytopenia (FNAIT)?
◍ normal saline.
Answer: Washed red blood cells are prepared by washing red cells with?
◍ Which of the following organs is responsible for the maturation of T
lymphocytes and regulation of their expression of CD4 and CD8?a. Spleenb.
Liverc. Thymusd. Bone marrow.
Answer: c. Thymus
◍ The best source of active bone marrow from a 20-year-old would be:a. Iliac
crestb. Femurc. Distal radius d. Tibia.
Answer: a. Iliac crest
◍ Physiologic programmed cell death is termed:a. Angiogenesisb. Apoptosisc.
Aneurysmd. Apohematics.
Answer: b. Apoptosis
◍ Albumin.
Answer: Which of these is a plasma derivative?
◍ to detect fetomaternal hemorrage in postpartum women.
Answer: What is the purpose of the rosette test?
◍ Which organ is the site of sequestration of platelets?a. Liver b. Thymusc.
Spleend. Bone marrow.
Answer: c. Spleen
◍ Which one of the following cells is a product of the CLP?a.
, Megakaryocyteb. T lymphocytec. Erythrocyted. Granulocyte.
Answer: b. T lymphocyte
◍ Rh-mother, Rh-fetus.
Answer: Which of these situations could result in hemolytic disease of the
newborn?
◍ Pink.
Answer: Which vacutainer tube is preferred for blood bank tests?
◍ What growth factor is produced in the kidneys and is used to treat anemia
associated with kidney disease?a. EPOb. TPOc. G-CSFd. KIT ligand.
Answer: a. EPO
◍ Which one of the following cytokines is required very early in the
differentiation of a hematopoietic stem cell?a. IL-2b. IL-8c. EPOd. FLT3
ligand.
Answer: d. FLT3 ligand
◍ 2.
Answer: Refer to the pedigree chart below:How many of the four children
would be expected to have type AB blood?
◍ Hematopoietic stem cells produce all lineages of blood cells in sufficient
quantities over the lifetime of an individual because they:a. Are unipotentb.
Have the ability of self-renewal by asymmetric divisionc. Are present in
large numbers in the bone marrow nichesd. Have a low mitotic potential in
response to growth factors.
Answer: b. Have the ability of self-renewal by asymmetric division
◍ Porcine.
Answer: A patient has hemophilia A and needs injections of factor VIII.
However, the patient's body has developed inhibitors to human factor VIII.
Which form of factor VIII could be given to this patient instead?
◍ by destroying fetal red blood cells in the mother's bloodstream.
Answer: How does anti-D immunoglobulin prevent Rhesus disease?
