ASCP CG LATEST EXAMS SCRIPT QUESTIONS
AND ANSWERS SURE A+
✔✔The dark bands produced by routune GTG banding represent: - ✔✔AT-rich regions
of the chromosome
aka heterpchromatin, and gene-poor chromatin, late replicating DNA
✔✔If GTG banded chromosomes appear ghost-like with little visible banding, one
should alter banding prep by: - ✔✔Decreasing trypsin time (chewed out and non-
distinct)
✔✔If 50 out of 200 interphase nuclei have 3 signals in a prenatal aneusomy FISH
analysis for chromosome 18, the results are: - ✔✔Consistent with trisomy 18 or a
structural anomaly of chromosome 18
✔✔If the same hybridization incubator is used for several different probe strategies, the
stringency for each probe can be adjusted by: - ✔✔adjusting the formamide and salt
concentrations
✔✔Optimal (best) banding quality is achieved for each patient sample by banding: -
✔✔one trial slide per patient to ensure BEST quality
, ✔✔In fluorescent microscopy, the exciter and barrier filters used depend on ___ ? -
✔✔Stain - because the wavelength for emission can vary per type of fluorescent stain
used (i.e. DAPI)
✔✔What is a possible reason a metaphase cannot be located when switching from the
40x lens to the 100x oil lens? - ✔✔The 100x objective is slightly unscrewed (can occur
when cleaning the scope)
✔✔What is an action a technologist can take to reduce the risk of repetitve motion
injuries when doing analysis on a computer? - ✔✔Supporting the arm that moves the
mouse,
Standing and moving around often, and
alternating hands while operating the computer mouse
✔✔What is the correct modal number for a patient with Turner syndrome with an
isochromosome Xq? - ✔✔46 -- because the isochromosome replaces the normal X,
causing Turner syndrome
✔✔What should be used on a cultured CVS sample to obtain more slides for analysis? -
✔✔Trypsin-EDTA -- to remove adherent cells from the flask or coverslip to split into
more vessels
EDTA improves tryp's effectiveness (chelating agent)
✔✔Cyto studies were ordered on a prenatal patient at 12 weeks gestation after an
abnormality was detected via ultrasound. 2 of 15 colonies from seperate primary
cultures showed an abnormal cell line of 47,XX,+21. The next step would be to ___ ? -
✔✔Report the result as this is a true mosaic case and suggest:
repeating amniocentesis,
repeating a detailed ultrasound, or
request percutaneous umbilical blood sample (PUBS)
✔✔After staining, the chromosomes appear to have indistinct bands with little contrast.
The problem is corrected by: - ✔✔Increasing trypsin exposure (chroms were muddy and
dark)
✔✔When scoring FISH for DiGeorge syndrome, a tech notices the TUPLE1 signal is
present, but one of the control ARSA probe signals is missing in every cell. This result
suggests __ ? - ✔✔The patient has a deletion of the ARSA region
✔✔CLIA 1988- (Clinical Laboratory Improvement Amendments) stipulates that
equipment must be recalibrated __ ? - ✔✔Every 6 months (Standard 493.1217)
AND ANSWERS SURE A+
✔✔The dark bands produced by routune GTG banding represent: - ✔✔AT-rich regions
of the chromosome
aka heterpchromatin, and gene-poor chromatin, late replicating DNA
✔✔If GTG banded chromosomes appear ghost-like with little visible banding, one
should alter banding prep by: - ✔✔Decreasing trypsin time (chewed out and non-
distinct)
✔✔If 50 out of 200 interphase nuclei have 3 signals in a prenatal aneusomy FISH
analysis for chromosome 18, the results are: - ✔✔Consistent with trisomy 18 or a
structural anomaly of chromosome 18
✔✔If the same hybridization incubator is used for several different probe strategies, the
stringency for each probe can be adjusted by: - ✔✔adjusting the formamide and salt
concentrations
✔✔Optimal (best) banding quality is achieved for each patient sample by banding: -
✔✔one trial slide per patient to ensure BEST quality
, ✔✔In fluorescent microscopy, the exciter and barrier filters used depend on ___ ? -
✔✔Stain - because the wavelength for emission can vary per type of fluorescent stain
used (i.e. DAPI)
✔✔What is a possible reason a metaphase cannot be located when switching from the
40x lens to the 100x oil lens? - ✔✔The 100x objective is slightly unscrewed (can occur
when cleaning the scope)
✔✔What is an action a technologist can take to reduce the risk of repetitve motion
injuries when doing analysis on a computer? - ✔✔Supporting the arm that moves the
mouse,
Standing and moving around often, and
alternating hands while operating the computer mouse
✔✔What is the correct modal number for a patient with Turner syndrome with an
isochromosome Xq? - ✔✔46 -- because the isochromosome replaces the normal X,
causing Turner syndrome
✔✔What should be used on a cultured CVS sample to obtain more slides for analysis? -
✔✔Trypsin-EDTA -- to remove adherent cells from the flask or coverslip to split into
more vessels
EDTA improves tryp's effectiveness (chelating agent)
✔✔Cyto studies were ordered on a prenatal patient at 12 weeks gestation after an
abnormality was detected via ultrasound. 2 of 15 colonies from seperate primary
cultures showed an abnormal cell line of 47,XX,+21. The next step would be to ___ ? -
✔✔Report the result as this is a true mosaic case and suggest:
repeating amniocentesis,
repeating a detailed ultrasound, or
request percutaneous umbilical blood sample (PUBS)
✔✔After staining, the chromosomes appear to have indistinct bands with little contrast.
The problem is corrected by: - ✔✔Increasing trypsin exposure (chroms were muddy and
dark)
✔✔When scoring FISH for DiGeorge syndrome, a tech notices the TUPLE1 signal is
present, but one of the control ARSA probe signals is missing in every cell. This result
suggests __ ? - ✔✔The patient has a deletion of the ARSA region
✔✔CLIA 1988- (Clinical Laboratory Improvement Amendments) stipulates that
equipment must be recalibrated __ ? - ✔✔Every 6 months (Standard 493.1217)
