Ace the ASCP Questions and answers University of
Arkansas for Medical Sciences MLSC 41381
Blood smear below denotes Hairy Cell Leukemia (HCL), which of the
following test is used to identify this type of leukemia?
Image. 1
Myeloperoxidase
Tartrate-resistant acid phosphatase
Tartrate-resistant alkaline phosphatase
Sudan Black
B. Tartrate-resistant acid phosphatase
Feedback:
Hairy cells demonstrate strong positivity for tartrate-resistant acid
phosphatase (TRAP) staining. The hairy cells are larger than normal and
positive for CD19, CD20, CD22, CD11c, CD25, CD103, and FMC7.
,Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC
ACID CONTAMINATION.
A True (Correct Answer)
B False
A. True
What disease is associated with the possession of HLA-
B27? Ankylosing spondylitis
Hashimoto's Disease
Goodpasture's
syndrome SLE
A. Ankylosing spondylitis
Feedback:
Ankylosing spondylitis belongs to a group of arthritis conditions
that tend to cause chronic inflammation of the spine
(spondyloarthropathies). The HLA-B27 gene can be detected in the
blood of most patients with ankylosing spondylitis.
,Burr cells are seen in the peripheral blood smear, this is indicative of? (Fig.
2)
Uremia (Kidney Disease)
Anemia
Leukemia
Liver Disease
A. Uremia (kidney Disease)
Feedback:
The above shown picture illustrates Burr cells, these cells are indicative of
Uremia (kidney disease)
, Anti IgG (-); C3d (+): What should the MLS do?
Elution
IAT
DAT
Pre-warm
D. Pre-warm
Feedback Prewarm technique can be used to prevent cold-reactive
alloantibodies or autoantibodies from reacting in the IAT phase. Specifically,
prewarm technique prevents cold antibodies from binding complement at
RT (as opposed to 37o C ) and subsequently being detected by anti-C3 in
the IAT by polyspecific AHG serum.
Arkansas for Medical Sciences MLSC 41381
Blood smear below denotes Hairy Cell Leukemia (HCL), which of the
following test is used to identify this type of leukemia?
Image. 1
Myeloperoxidase
Tartrate-resistant acid phosphatase
Tartrate-resistant alkaline phosphatase
Sudan Black
B. Tartrate-resistant acid phosphatase
Feedback:
Hairy cells demonstrate strong positivity for tartrate-resistant acid
phosphatase (TRAP) staining. The hairy cells are larger than normal and
positive for CD19, CD20, CD22, CD11c, CD25, CD103, and FMC7.
,Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC
ACID CONTAMINATION.
A True (Correct Answer)
B False
A. True
What disease is associated with the possession of HLA-
B27? Ankylosing spondylitis
Hashimoto's Disease
Goodpasture's
syndrome SLE
A. Ankylosing spondylitis
Feedback:
Ankylosing spondylitis belongs to a group of arthritis conditions
that tend to cause chronic inflammation of the spine
(spondyloarthropathies). The HLA-B27 gene can be detected in the
blood of most patients with ankylosing spondylitis.
,Burr cells are seen in the peripheral blood smear, this is indicative of? (Fig.
2)
Uremia (Kidney Disease)
Anemia
Leukemia
Liver Disease
A. Uremia (kidney Disease)
Feedback:
The above shown picture illustrates Burr cells, these cells are indicative of
Uremia (kidney disease)
, Anti IgG (-); C3d (+): What should the MLS do?
Elution
IAT
DAT
Pre-warm
D. Pre-warm
Feedback Prewarm technique can be used to prevent cold-reactive
alloantibodies or autoantibodies from reacting in the IAT phase. Specifically,
prewarm technique prevents cold antibodies from binding complement at
RT (as opposed to 37o C ) and subsequently being detected by anti-C3 in
the IAT by polyspecific AHG serum.
