ACS BIOCHEMISTRY PRACTICE FINAL
FGCU BEHARRY COTICONE PACKAGE
DEAL STUDY GUIDE 2026
COMPREHENSIVE QUESTIONS WITH
EXPERT SOLUTIONS GRADED A+
⩥ ATP Producing Steps of Glycolysis.
Answer: Steps 7 and 10.
1,3-bisphosphoglycerate <--> 3-phosphoglycerate
PEP --> Pyruvate
⩥ NADH Producing Step of Glycolysis.
Answer: Step 6
G3P <--> 1,3-bisphosphoglycerate
⩥ Total Energy Produced by Glycolysis.
Answer: 2NADH + 4 ATP
⩥ Lactic Acid Fermentation.
Answer: Pyruvate --> L-Lactate
NADH --> NAD+
,Regenerates NAD+ for use in glycolysis
⩥ Ethanol Fermentation.
Answer: Pyruvate --> Acetalaldehyde --> Ethanol
Uses pyruvate decarboxylase (TPP) and alcohol dehydrogenase.
NADH --> CO2(TPP) + NAD+
⩥ TPP Cofactor Structure.
Answer:
⩥ TPP Cofactor.
Answer: Common acetaldehyde carrier. Used in pyruvate decarboxylase,
pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and
transketolase
⩥ Bypass Reactions in Gluconeogenesis.
Answer: Steps 1,3, and 10 must be bypassed.
⩥ Gluconeogenic Bypass of Step 10.
Answer: Bicarbonate + Pyruvate --> Oxaloacetate
Pyruvate decarboxylate (biotin)
ATP --> ADP
, Oxaloacetate --> PEP
PEP carboxykinase
GTP --> GDP + CO2
⩥ Gluconeogenic Bypass of Step 3.
Answer: Fructose 1,6-bisphosphate + H2O --> Fructose 6-phosphate +
Pi
Uses FBPase-1 (coordinated with PFK-1)
⩥ Gluconeogenic Bypass of Step 1.
Answer: Glucose 6-phosphate + H2O --> Glucose + Pi
Uses glucose 6-phosphatase.
⩥ Cost of Gluconeogenesis.
Answer: 4 ATP, 2 GTP, and 2 NADH
⩥ Oxidative Pentose Phosphate Pathway.
Answer: Uses glucose 6-phosphate to produce 2 NADPH and ribose 5-
phosphate used for biosynthesis
⩥ Non-Oxidative Pentose Phosphate Pathway.
FGCU BEHARRY COTICONE PACKAGE
DEAL STUDY GUIDE 2026
COMPREHENSIVE QUESTIONS WITH
EXPERT SOLUTIONS GRADED A+
⩥ ATP Producing Steps of Glycolysis.
Answer: Steps 7 and 10.
1,3-bisphosphoglycerate <--> 3-phosphoglycerate
PEP --> Pyruvate
⩥ NADH Producing Step of Glycolysis.
Answer: Step 6
G3P <--> 1,3-bisphosphoglycerate
⩥ Total Energy Produced by Glycolysis.
Answer: 2NADH + 4 ATP
⩥ Lactic Acid Fermentation.
Answer: Pyruvate --> L-Lactate
NADH --> NAD+
,Regenerates NAD+ for use in glycolysis
⩥ Ethanol Fermentation.
Answer: Pyruvate --> Acetalaldehyde --> Ethanol
Uses pyruvate decarboxylase (TPP) and alcohol dehydrogenase.
NADH --> CO2(TPP) + NAD+
⩥ TPP Cofactor Structure.
Answer:
⩥ TPP Cofactor.
Answer: Common acetaldehyde carrier. Used in pyruvate decarboxylase,
pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and
transketolase
⩥ Bypass Reactions in Gluconeogenesis.
Answer: Steps 1,3, and 10 must be bypassed.
⩥ Gluconeogenic Bypass of Step 10.
Answer: Bicarbonate + Pyruvate --> Oxaloacetate
Pyruvate decarboxylate (biotin)
ATP --> ADP
, Oxaloacetate --> PEP
PEP carboxykinase
GTP --> GDP + CO2
⩥ Gluconeogenic Bypass of Step 3.
Answer: Fructose 1,6-bisphosphate + H2O --> Fructose 6-phosphate +
Pi
Uses FBPase-1 (coordinated with PFK-1)
⩥ Gluconeogenic Bypass of Step 1.
Answer: Glucose 6-phosphate + H2O --> Glucose + Pi
Uses glucose 6-phosphatase.
⩥ Cost of Gluconeogenesis.
Answer: 4 ATP, 2 GTP, and 2 NADH
⩥ Oxidative Pentose Phosphate Pathway.
Answer: Uses glucose 6-phosphate to produce 2 NADPH and ribose 5-
phosphate used for biosynthesis
⩥ Non-Oxidative Pentose Phosphate Pathway.