BIOD 171 ESSENTIAL MICROBIOLOGY PORTAGE
LEARNING MODULE 4 EXAM WITH SOLUTIONS
What are the requirements of fastidious microbe?
A fastidious microbe is an organism with complex growth
requirements such that if absent it will not grow. Enriched
medias thus contain these specific and essential nutrients
required for the growth of a particular subset of
microorganisms.
True or False: Differential media is best suited for
distinguishing between two similar species of
bacteria.
True
(Growth media does not contain restrictive factors, while
selective media is best used to encourage the growth of
one microbe.)
True or False: Growth media is best suited for
distinguishing between two similar species of
bacteria.
,False
(This is differential media)
(Growth media is designed to simply support (and not
restrict) microbial growth).
A researcher is asked to determine if a sample
contains Neisseria meningitides.
Knowing Neisseria meningitides is slow growing and
other foreign microbes may also be present in the
culture, which type of media would be best suited?
A. Growth Media
B. Selective Media
C. Selective and Differential Media
D. Differential Media
B. Selective media
(If additional foreign microbes may be present in your
sample and you know Neisseria is slow growing, you must
restrict the unwanted microbes while supporting the
growth of Neisseria meningitides. Thus, you are
, selectively encouraging one microbe's growth, while
selectively restricting others).
A researcher is asked to determine which two vials
contains E. coli and which contains salmonella.
Knowing both are Gram Negative while only one of
them is capable of fermenting lactose, which type of
media would be best suited?
A. Growth Media
B. Differential Media
C. Selective Media
D. Selective and Differential Meida
Differential media
(Differential media distinguishes between two, often
related, microbes).
True or False: LB agar is classified as a selective,
non-differential media.
False
(LB agar is non-selective and non-differential).
LEARNING MODULE 4 EXAM WITH SOLUTIONS
What are the requirements of fastidious microbe?
A fastidious microbe is an organism with complex growth
requirements such that if absent it will not grow. Enriched
medias thus contain these specific and essential nutrients
required for the growth of a particular subset of
microorganisms.
True or False: Differential media is best suited for
distinguishing between two similar species of
bacteria.
True
(Growth media does not contain restrictive factors, while
selective media is best used to encourage the growth of
one microbe.)
True or False: Growth media is best suited for
distinguishing between two similar species of
bacteria.
,False
(This is differential media)
(Growth media is designed to simply support (and not
restrict) microbial growth).
A researcher is asked to determine if a sample
contains Neisseria meningitides.
Knowing Neisseria meningitides is slow growing and
other foreign microbes may also be present in the
culture, which type of media would be best suited?
A. Growth Media
B. Selective Media
C. Selective and Differential Media
D. Differential Media
B. Selective media
(If additional foreign microbes may be present in your
sample and you know Neisseria is slow growing, you must
restrict the unwanted microbes while supporting the
growth of Neisseria meningitides. Thus, you are
, selectively encouraging one microbe's growth, while
selectively restricting others).
A researcher is asked to determine which two vials
contains E. coli and which contains salmonella.
Knowing both are Gram Negative while only one of
them is capable of fermenting lactose, which type of
media would be best suited?
A. Growth Media
B. Differential Media
C. Selective Media
D. Selective and Differential Meida
Differential media
(Differential media distinguishes between two, often
related, microbes).
True or False: LB agar is classified as a selective,
non-differential media.
False
(LB agar is non-selective and non-differential).
