WITH QUESTIONS AND VERIFIED ANSWERS
What is agar used for in microbiology? -
answer-Agar is used to create a solid, smooth
surface on which microbes can grow.
In Eosin Methylene Blue (EMB) agar, what color
indicates the presence of E. coli? - answer-
Metallic Green
In order to visualize individual colonies of
bacteria would you culture your sample in a
liquid
media or on a solid (agar) media? Why? -
answer-Solid (agar) media because the
primary advantage is that cells are held into
place. When grown on agar in a petri dish, it's
fixed in a way to form colonies. In a nutrient
,broth, bacterial cells can multiple but are able
to freely move around in the solution.
A dilution gradient is formed when carrying out
what generalize plating strategy? - answer-
Quadrant streak
True or False: Growth Media is best suited for
distinguishing between two similar species of
bacteria. - answer-FALSE. Growth media is
designed to simply support (and not restrict)
microbial growth
A researcher is asked to determine which of
two vitals contains E.coli and which contains
Salmonella. Knowing both are gram-negative
while only one of them is:
A. Growth Media
B. Differential Media
C. Selective Media
,D. Selective and Differential media - answer-
Differential Media (distinguishes between two
or more microbes that are related)
What re the requirements of a fastidious
microbe? - answer-Enriched medias that
contain specific and essential nutrients that
are required for growth
True or False: LB agar is classified as a
selective, non-differential media. - answer-
FALSE.
What is agar used for in microbiology? -
answer-To create a solid, smooth surface on
which microbes can grow
Match the following hemolytic class with its
description
A. No hemolytic activity
, B. Incomplete hemolytic activity
C. Complete hemolytic activity - answer-Alpha:
Incomplete hemolytic activity
Beta: Complete hemolytic activity
Gamma: No hemolytic activity
Columbia CNA agar is most closely related to
which media:
A. TSA/TYSAE Agar
B. MacConkey Agar
C. BAP
D. EMB Agar - answer-BAP ( both are enriched
with blood)
True or False: Chocolate agar gets its brown
color from cocoa to produce an enriched
media. - answer-FALSE. (Chocolate (cocoa) is
never added to the media)