UAMS-Microbiology Exam 4 Latest Questions
and Answers (2026) | Updated Comprehensive
Review with Detailed Rationales | A+ Verified
• Acridine orange stain -✓✓-Acridine orange stains nucleic acid of living and dead cells
-Bacteria and yeast
• What plates do we subculture CSF?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, Thio
-37°C in CO2
• What are common CSF pathogens? -✓✓-H. influenzae
-S. pneumoniae
-N. meningitidis
-GBS
-Listeria
-CNS
• What is CSF rhinorrhea? -✓✓CSF leaking through nasal passage due to blunt trauma,
skull fracture or a tumor
• What plates do we subculture abscess's and wound cultures?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, MAC/EMB, CNA/PEA
-anaerobic if requested
-37°C in CO2
***Pus is the best specimen***
• What are common abscess and wound culture pathogens? -✓✓-Staph Aureus
-GAS
-Enterics
-Anaerobes
-PSAR
-Enterococci
• What plates do we subculture stool?
What temperature and atmosphere is needed? -✓✓-BAP, MAC/EMB, HE/SS/XLD, GN
broth
-37°C in air
• Special stool culture plates -✓✓CampyBAP, CIN, TCBS, SMAC
• What plates do we subculture genital specimens?
What temperature and atmosphere is needed? -✓✓-BAP, MTM
, -37°C in CO2
-Gram stain genital specimens and look for the following; clue cells, gram negative
diplococci, yeast
• What plates do we subculture sinus specimens?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, broth, anaerobic
media
-37°C in CO2
• What are common sinus pathogens? -✓✓-S. pneumoniae
-M. catarrhalis
-H. influenzae
• What plates do we subculture otitis media specimens?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, broth, anaerobic
media
-35°C in CO2
• What are otitis media common pathogens? -✓✓-H. influenzae
-S. pneumoniae
-M. catarrhalis
• What plates do we subculture otitis externa specimens?
What temperature and atmosphere is needed? -✓✓-BAP, MAC/EMB
-35°C in air
-"swimmers ear"
• What is the most common pathogen that causes otitis externa? -✓✓PSAR
• What plates do we subculture epiglottis specimens?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, MAC
-35°C in CO2
• What are the common pathogens for epiglottis specimens? -✓✓-H. influenzae(type B)
-S. pneumoniae
• What plates do we subculture lower respiratory tract specimens(LRT)?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, EMB/MAC, broth
• Special media used for lower respiratory tract? -✓✓BCYE, BCSA/OFPBL, Regan
Lowe
• How to determine if a LRT specimen is good quality? -✓✓↑ PMN's and ↓ Sq epi =
good specimen
↓ PMN's and ↑ Sq epi = bad specimen
and Answers (2026) | Updated Comprehensive
Review with Detailed Rationales | A+ Verified
• Acridine orange stain -✓✓-Acridine orange stains nucleic acid of living and dead cells
-Bacteria and yeast
• What plates do we subculture CSF?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, Thio
-37°C in CO2
• What are common CSF pathogens? -✓✓-H. influenzae
-S. pneumoniae
-N. meningitidis
-GBS
-Listeria
-CNS
• What is CSF rhinorrhea? -✓✓CSF leaking through nasal passage due to blunt trauma,
skull fracture or a tumor
• What plates do we subculture abscess's and wound cultures?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, MAC/EMB, CNA/PEA
-anaerobic if requested
-37°C in CO2
***Pus is the best specimen***
• What are common abscess and wound culture pathogens? -✓✓-Staph Aureus
-GAS
-Enterics
-Anaerobes
-PSAR
-Enterococci
• What plates do we subculture stool?
What temperature and atmosphere is needed? -✓✓-BAP, MAC/EMB, HE/SS/XLD, GN
broth
-37°C in air
• Special stool culture plates -✓✓CampyBAP, CIN, TCBS, SMAC
• What plates do we subculture genital specimens?
What temperature and atmosphere is needed? -✓✓-BAP, MTM
, -37°C in CO2
-Gram stain genital specimens and look for the following; clue cells, gram negative
diplococci, yeast
• What plates do we subculture sinus specimens?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, broth, anaerobic
media
-37°C in CO2
• What are common sinus pathogens? -✓✓-S. pneumoniae
-M. catarrhalis
-H. influenzae
• What plates do we subculture otitis media specimens?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, broth, anaerobic
media
-35°C in CO2
• What are otitis media common pathogens? -✓✓-H. influenzae
-S. pneumoniae
-M. catarrhalis
• What plates do we subculture otitis externa specimens?
What temperature and atmosphere is needed? -✓✓-BAP, MAC/EMB
-35°C in air
-"swimmers ear"
• What is the most common pathogen that causes otitis externa? -✓✓PSAR
• What plates do we subculture epiglottis specimens?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, MAC
-35°C in CO2
• What are the common pathogens for epiglottis specimens? -✓✓-H. influenzae(type B)
-S. pneumoniae
• What plates do we subculture lower respiratory tract specimens(LRT)?
What temperature and atmosphere is needed? -✓✓-BAP, CHOC, EMB/MAC, broth
• Special media used for lower respiratory tract? -✓✓BCYE, BCSA/OFPBL, Regan
Lowe
• How to determine if a LRT specimen is good quality? -✓✓↑ PMN's and ↓ Sq epi =
good specimen
↓ PMN's and ↑ Sq epi = bad specimen