GMS 6440 Module 5 Notes 2026 – Complete
Review & Exam Prep Guide
1. To generate conventional transgenic mice, DNA will be injected into: embry-
onic stem cells or fertilized mouse eggs: fertilized mouse eggs
2. To determine success of homologous recombination of targeting vector in
ES cells, Northern blotting will be utilized. Yes or No: No (southern blotting)
3. Using Cre recombinase, we can control DNA recombination and delete
specific genomic areas flanked by FLP sequence, True or False: false, Cre recognized
LoxP sites
4. tamoxifen, an estrogen antagonist, will bind to the mutated estrogen re-
ceptor fused to the Cre-recombinase and translocate Cre-recombinase into
nucleus. True or False: True
5. Cre-loxP system also can be used to eliminate floxed-stop codon and induce
expression of a transgene, true or false?: true
6. We cannot control copy number of inserted DNA as well as insertion site
(position) using the conventional transgenic approach. Insertion of DNA con-
struct into ROSA26 locus can be a solution to overcome these insertional
effects, true or false?: true
7. In the CRISPR/Cas9 system, a double-stranded break in the DNA is induced
by the enzyme, CRISPR.: False, the enzyme is Cas9
8. In the CRISPR/Cas9, guide RNA directs the enzyme to cut the DNA at a
specific site in the genome, true or false?: true
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Review & Exam Prep Guide
1. To generate conventional transgenic mice, DNA will be injected into: embry-
onic stem cells or fertilized mouse eggs: fertilized mouse eggs
2. To determine success of homologous recombination of targeting vector in
ES cells, Northern blotting will be utilized. Yes or No: No (southern blotting)
3. Using Cre recombinase, we can control DNA recombination and delete
specific genomic areas flanked by FLP sequence, True or False: false, Cre recognized
LoxP sites
4. tamoxifen, an estrogen antagonist, will bind to the mutated estrogen re-
ceptor fused to the Cre-recombinase and translocate Cre-recombinase into
nucleus. True or False: True
5. Cre-loxP system also can be used to eliminate floxed-stop codon and induce
expression of a transgene, true or false?: true
6. We cannot control copy number of inserted DNA as well as insertion site
(position) using the conventional transgenic approach. Insertion of DNA con-
struct into ROSA26 locus can be a solution to overcome these insertional
effects, true or false?: true
7. In the CRISPR/Cas9 system, a double-stranded break in the DNA is induced
by the enzyme, CRISPR.: False, the enzyme is Cas9
8. In the CRISPR/Cas9, guide RNA directs the enzyme to cut the DNA at a
specific site in the genome, true or false?: true
1/1
