ASCP MB Review EXAM (2026) UPDATE Verified
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Terms in this set (265)
Primary Structure Sequence of Amino Acids
Secondary Structure Side Chain Interactions
Ex. Alpha Helices and Beta-Pleated Sheets
Tertiary Structure Configuration of Folded Proteins; if Denatured,
Loses Function
Quaternary Structure Functional Association of Separate Proteins
UAA, UAG, UGA Nonsense/Termination Codons
Amino Acid Charging Catalyzed by Aminoacyl tRNA Synthetase; Mg+2
Dependent
AUG Start Codon (Methionine)
Size Exclusion Columns Porous Beads that exclude larger molecules and
retain smaller molecules
, Single Strand Conformation Short ds PCR products denatured then rapidly
Polymorphism (SSCP) cooled; fold into conformers; resolve on gel;
mutations cause different conformation and
migration patterns
Denaturing Gradient Gel dsDNA fragments separated on PAGE with
Electrophoresis (DGGE) gradient concentration of urea and formamide
Sequence Specific Primer PCR 3' Primer end falls on nucleotide to be analyzed;
(SSP-PCR) 3' end will need to match the template to be
extended by Taq; the presence/absence of
product = presence/absence of mutation
Size of the Human Genome 2.9 Billion bp
Polymorphism Change in DNA sequence present in at least 1-2%
of the population
Alpha Satellite Highly repetitive sequences at the centromere,
these interfere with chromosome compaction
and therefore cause constriction at centromere
Metacentric Chromosomal arms equal in length
Sub-Metacentric Q arm is longer than the P arm
Acrocentric P arm is extremely small and the Q arm is of
normal size
Telocentric There is only presence of the Q arm
Questions And Answers | With 100% Correct
Answers graded A+ Guaranteed Success!!
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Terms in this set (265)
Primary Structure Sequence of Amino Acids
Secondary Structure Side Chain Interactions
Ex. Alpha Helices and Beta-Pleated Sheets
Tertiary Structure Configuration of Folded Proteins; if Denatured,
Loses Function
Quaternary Structure Functional Association of Separate Proteins
UAA, UAG, UGA Nonsense/Termination Codons
Amino Acid Charging Catalyzed by Aminoacyl tRNA Synthetase; Mg+2
Dependent
AUG Start Codon (Methionine)
Size Exclusion Columns Porous Beads that exclude larger molecules and
retain smaller molecules
, Single Strand Conformation Short ds PCR products denatured then rapidly
Polymorphism (SSCP) cooled; fold into conformers; resolve on gel;
mutations cause different conformation and
migration patterns
Denaturing Gradient Gel dsDNA fragments separated on PAGE with
Electrophoresis (DGGE) gradient concentration of urea and formamide
Sequence Specific Primer PCR 3' Primer end falls on nucleotide to be analyzed;
(SSP-PCR) 3' end will need to match the template to be
extended by Taq; the presence/absence of
product = presence/absence of mutation
Size of the Human Genome 2.9 Billion bp
Polymorphism Change in DNA sequence present in at least 1-2%
of the population
Alpha Satellite Highly repetitive sequences at the centromere,
these interfere with chromosome compaction
and therefore cause constriction at centromere
Metacentric Chromosomal arms equal in length
Sub-Metacentric Q arm is longer than the P arm
Acrocentric P arm is extremely small and the Q arm is of
normal size
Telocentric There is only presence of the Q arm
