Acetic acid - ANSWER This fixative does not fix or destroy carbohydrates, and it does not
fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative
mixtures because of its ability to fix nuclei.
Glutaraldehyde - ANSWER Techniques using Schiff reagent, such as the periodic acid-
Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results
will be obtained.
Glyoxal - ANSWER This fixative is the smallest dialdehyde and is used in the textile indus-
try for imparting wrinkle resistance and permanent press creases. It has replaced formalde-
hyde in some histopathology laboratories because it is much less toxic.
Zinc salts - ANSWER Today, _________ has/have found use not only in combination with
formaldehyde for routine fixation, but as a substitute for mercury in the B-5 solution com-
monly used for the fixation of lymph node and bone marrow tissues
Michel transport medium - ANSWER If unfixed tissue is to be held for several days or
transported over a long distance then this transport medium is recommended.
Dehydration, clearing, infiltration - ANSWER Tissue processing is usually considered to in-
clude:
Xylene - ANSWER The most widely used clearing agent in histology today is:
Ethylenediaminetetraacetic acid (EDTA) - ANSWER This chelating agent binds calcium ions
and is one method used for decalcifying tissues.
1
, Phase-contrast microscope - ANSWER This microscope is used for the examination of un-
stained specimens, especially unstained living cells, and allows almost transparent objects to
be seen clearly.
Cryostat - ANSWER This piece of equipment is a refrigerated chamber containing a micro-
tome
that is used to cut frozen sections.
Hazardous chemicals - ANSWER OSHA defines ____________ as chemicals that may
cause acute or chronic health effects in exposed employees.
Toxic dose low - ANSWER The lowest dose of a substance that will produce any toxic ef-
fect in humans when introduced by any route other than inhalation is the:
Reactivity - ANSWER The right yellow diamond in the NFPA coded labeling system carries
a chemical's ____________ information.
Nucleolus - ANSWER This dense, rounded, and usually intensely basophilic mass in the
nucleus of
a cell consists of 80% to 90% protein and produces most if not all of the ribosomal RNA.
Ribosomes - ANSWER These cell structures are often found attached to the endoplasmic
reticulum
and are the site of protein synthesis.
Hematoxylin - ANSWER The most widely used nuclear stain for routine histology sections
is:
PAS with diastase digestion - ANSWER The main purpose of this stain is to demonstrate
glycogen in tissue sections.
2
fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative
mixtures because of its ability to fix nuclei.
Glutaraldehyde - ANSWER Techniques using Schiff reagent, such as the periodic acid-
Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results
will be obtained.
Glyoxal - ANSWER This fixative is the smallest dialdehyde and is used in the textile indus-
try for imparting wrinkle resistance and permanent press creases. It has replaced formalde-
hyde in some histopathology laboratories because it is much less toxic.
Zinc salts - ANSWER Today, _________ has/have found use not only in combination with
formaldehyde for routine fixation, but as a substitute for mercury in the B-5 solution com-
monly used for the fixation of lymph node and bone marrow tissues
Michel transport medium - ANSWER If unfixed tissue is to be held for several days or
transported over a long distance then this transport medium is recommended.
Dehydration, clearing, infiltration - ANSWER Tissue processing is usually considered to in-
clude:
Xylene - ANSWER The most widely used clearing agent in histology today is:
Ethylenediaminetetraacetic acid (EDTA) - ANSWER This chelating agent binds calcium ions
and is one method used for decalcifying tissues.
1
, Phase-contrast microscope - ANSWER This microscope is used for the examination of un-
stained specimens, especially unstained living cells, and allows almost transparent objects to
be seen clearly.
Cryostat - ANSWER This piece of equipment is a refrigerated chamber containing a micro-
tome
that is used to cut frozen sections.
Hazardous chemicals - ANSWER OSHA defines ____________ as chemicals that may
cause acute or chronic health effects in exposed employees.
Toxic dose low - ANSWER The lowest dose of a substance that will produce any toxic ef-
fect in humans when introduced by any route other than inhalation is the:
Reactivity - ANSWER The right yellow diamond in the NFPA coded labeling system carries
a chemical's ____________ information.
Nucleolus - ANSWER This dense, rounded, and usually intensely basophilic mass in the
nucleus of
a cell consists of 80% to 90% protein and produces most if not all of the ribosomal RNA.
Ribosomes - ANSWER These cell structures are often found attached to the endoplasmic
reticulum
and are the site of protein synthesis.
Hematoxylin - ANSWER The most widely used nuclear stain for routine histology sections
is:
PAS with diastase digestion - ANSWER The main purpose of this stain is to demonstrate
glycogen in tissue sections.
2
