An example of an additive fixative is one that contains: - ANSWER picric acid
Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear
bubbling. One most often sees this artifact if the specimen is processed following: - AN-
SWER incomplete fixation
Microscopic evalution of H&E stained sections from a surgically removed small bowl speci-
men shows an absence of much of the epithelium in otherwise normal tissue. This most
likely resulted from: - ANSWER delayed fixation
When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show: - AN-
SWER superior nuclear detail
A certain project requires a fixative that contains acetic acid yet stabilizes erythrocyte mem-
branes. One fixative that could be used is: - ANSWER Hollande solution
A specimen of kidney must be shipped to another city for immunofluorescence studies. The
specimen should be placed in: - ANSWER Michel Solution
When the microwave oven is used for fixation, the most critical factor is the: - ANSWER
control of temperature
To adequately remove the calcium from a specimen containing areas of microcalcification,
the tissue should be fixed in: - ANSWER Hollande solution
Which of the following fixatives contains copper acetate? - ANSWER Hollande
1
, A specimen is submitted with the statement that it was fixed in formalin. Microscopic sec-
tions show marked lysis of erythrocytes. This indicates that the fixative most likely was: - AN-
SWER acidified with acetic acid
Fixatives are classified as additive because of the: - ANSWER addition, or binding of the
fixative to tissue proteins.
Kidney biopsy tissue has been fixed in phosphate-buffered glutaraldehyde for 2 hours and
then placed in phosphate buffer solution. If a portion of this tisssue is processed for light mi-
croscopy, sections would most likely show: - ANSWER nonspecific PAS staining
Uric acid crystals are preserved ONLY when tissue is fixed in: - ANSWER absolute alcohol
Improper preservation of tissue will result if there is: - ANSWER a delay in fixation
A good fixative will: - ANSWER protect tissue against alteration during subsequent pro-
cessing.
The function of methanol in commercial formalin solutions is to: - ANSWER retard the
polymerization of formaldehyde
In electron microscopy, Zamboni fluid, glutaraldehyde, and osmium tetroxide function as: -
ANSWER fixative solutions
Tissue will remain unfixed if placed in: - ANSWER sodium borate
Bouin Solution is contraindicated for: - ANSWER tissue to be stained by the Feulgen reac-
tion
2
Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear
bubbling. One most often sees this artifact if the specimen is processed following: - AN-
SWER incomplete fixation
Microscopic evalution of H&E stained sections from a surgically removed small bowl speci-
men shows an absence of much of the epithelium in otherwise normal tissue. This most
likely resulted from: - ANSWER delayed fixation
When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show: - AN-
SWER superior nuclear detail
A certain project requires a fixative that contains acetic acid yet stabilizes erythrocyte mem-
branes. One fixative that could be used is: - ANSWER Hollande solution
A specimen of kidney must be shipped to another city for immunofluorescence studies. The
specimen should be placed in: - ANSWER Michel Solution
When the microwave oven is used for fixation, the most critical factor is the: - ANSWER
control of temperature
To adequately remove the calcium from a specimen containing areas of microcalcification,
the tissue should be fixed in: - ANSWER Hollande solution
Which of the following fixatives contains copper acetate? - ANSWER Hollande
1
, A specimen is submitted with the statement that it was fixed in formalin. Microscopic sec-
tions show marked lysis of erythrocytes. This indicates that the fixative most likely was: - AN-
SWER acidified with acetic acid
Fixatives are classified as additive because of the: - ANSWER addition, or binding of the
fixative to tissue proteins.
Kidney biopsy tissue has been fixed in phosphate-buffered glutaraldehyde for 2 hours and
then placed in phosphate buffer solution. If a portion of this tisssue is processed for light mi-
croscopy, sections would most likely show: - ANSWER nonspecific PAS staining
Uric acid crystals are preserved ONLY when tissue is fixed in: - ANSWER absolute alcohol
Improper preservation of tissue will result if there is: - ANSWER a delay in fixation
A good fixative will: - ANSWER protect tissue against alteration during subsequent pro-
cessing.
The function of methanol in commercial formalin solutions is to: - ANSWER retard the
polymerization of formaldehyde
In electron microscopy, Zamboni fluid, glutaraldehyde, and osmium tetroxide function as: -
ANSWER fixative solutions
Tissue will remain unfixed if placed in: - ANSWER sodium borate
Bouin Solution is contraindicated for: - ANSWER tissue to be stained by the Feulgen reac-
tion
2
