HISTOLOGY LECTURE EXAM 1
QUESTIONS & ANSWERS
0.001mm is equivalent to - Correct Answers -1 micron
A cryostat is a special type of microtome used in surgical analysis to produce frozen
tissue sections. - Correct Answers -True
A compound light microscope normally found in student labs consists of a convex lens
known as the objective lens and a concave lens called the ocular lens. - Correct
Answers -False
An imaginary image is one that can only be viewed by looking through the lens such as
in a magnifying glass. - Correct Answers -True
An imaginary image is produced when the object to be viewed is placed inside the focal
point of the convex lens. - Correct Answers -True
A transmission electron microscope employs an electron beam which passes through a
tissue section and is captured on a photographic plate for a permanent record - Correct
Answers -True
A scanning electron microscope is used when the electron beam passes through the
thin tissue section in a fast scanning motion - Correct Answers -False
A special microtome able to produce very thin sections must be used for tissue samples
to be used with the electron microscope for the following reason: - Correct Answers -To
permit the passage of electrons through the tissue section
An ultramicrotome can produce sections with a thickness of a 30-40 nm which is the
thickness needed for electron microscopy - Correct Answers -True
Choose the best answer which fits how a phase contrast microscope works - Correct
Answers -Utilizes small differences in the refractive index to visualize different cell parts
Light microscope sections must be thin for the following reason: - Correct Answers -To
more easily permit light to pass through the section
,Histology is primarily the study of the structure of cells, tissues, and organs and the
relationship of microscopic structure and function. - Correct Answers -True
In simple optics of a convex lens, an imaginary image is one that can be projected onto
a screen and viewed with our own eyes. - Correct Answers -False
In a compound microscope, the objective lens produces an image that falls outside the
focal point of the ocular lens. - Correct Answers -False
In an electron microscope, the glass lens must be of a special construction to permit the
electrons to pass through. - Correct Answers -False
If the object size is 1 microns, calculate the magnification produced using a 50x
objective lens and 10x ocular lens - Correct Answers -500 microns
In the paraffin technique for producing sections for microscopic study, which statement
describes the process of dehydration, clearing and embedding. - Correct Answers -The
fixed tissue is placed in successively stronger ethyl alcohol concentrations, xylene and
then paraffin
If you would like to produce a three dimensional image of a biological specimen, which
type of microscope listed would you use? - Correct Answers -Confocal scanning
microscope
Staining of a tissue section is necessary to provide contrast to cellular components to
make them visible in the light microscope. - Correct Answers -True
Tissues are composed solely of particular types of cells - Correct Answers -False
Our brains invert the image on our retina so that we can see the world right-side up -
Correct Answers -True
One Angstrom is equivalent to: - Correct Answers -None of the listed choices
Tissue structures absorbing basic stains are termed basophobic - Correct Answers -
False
One reason for chemical fixation of a tissue section is to curtail release from dead cells
of enzymes capable of digesting tissue constituents. - Correct Answers -True
What type of microscope can be used to quantitatively measure DNA and RNA in cells -
Correct Answers -Ultraviolet microscope
Which type of microscope can be used to study the surface topography at molecular
and atomic resolution? - Correct Answers -Atomic force microscope
, When applied to histologic sections, the term ___ means a flaw caused by faulty
technique - Correct Answers -Artifact
Brown fat mitochondria from bats do not have elementary particles and this helps them
to: - Correct Answers -Produce more heat to speed up the process of getting their body
temperature back up to normal at end of hibernation
An amphipathic molecule possesses a hydrophobic end but no portion of the molecule
is hydrophilic - Correct Answers -False
Microfilaments help cells change shape, exhibit motility and divide - Correct Answers -
True
What wavelength is used in the equation for calculating resolving power or resolution? -
Correct Answers -540 nm
Which type of microscopy is useful in examining urine for crystals of uric acid and
oxalate as well as observing for the presence of spirochetes such as Treponema
pallidum, the organism causing syphilis - Correct Answers -Dark field microscope
When an object is located inside the focal point of the ocular lens of a compound
microscope, choose the statement below that is true. - Correct Answers -The image is
magnified, erect, and imaginary
When an image is located outside the focal point of the objective lens of a compound
microscope, choose the statement below that is true. - Correct Answers -The image is
magnified, inverted and classified as a real image
What is the numerical aperture of a lens? - Correct Answers -The ability of a lens to
gather light
When slides are made from frozen tissues, the sections are cut inside a refrigerated
cabinet called a ___ - Correct Answers -Cryostat
The type of microscope which is able to see the three-dimensional distribution of a
specific constituent of a living tissue by the use of a narrow laser beam that can be
precisely focused to the appropriate depth is called a ___________________. - Correct
Answers -Confocal microscope
The stage in the paraffin technique for producing a tissue slide for viewing with a light
microscope that entails using a microtome to cut the paraffin embedded tissue into thin
slices is called _____________. - Correct Answers -Sectioning
The fluid content of the gelatinous interstitial matrix surrounding small blood vessels
which enables oxygen and nutrients to diffuse from the blood fluid inside capillaries to
QUESTIONS & ANSWERS
0.001mm is equivalent to - Correct Answers -1 micron
A cryostat is a special type of microtome used in surgical analysis to produce frozen
tissue sections. - Correct Answers -True
A compound light microscope normally found in student labs consists of a convex lens
known as the objective lens and a concave lens called the ocular lens. - Correct
Answers -False
An imaginary image is one that can only be viewed by looking through the lens such as
in a magnifying glass. - Correct Answers -True
An imaginary image is produced when the object to be viewed is placed inside the focal
point of the convex lens. - Correct Answers -True
A transmission electron microscope employs an electron beam which passes through a
tissue section and is captured on a photographic plate for a permanent record - Correct
Answers -True
A scanning electron microscope is used when the electron beam passes through the
thin tissue section in a fast scanning motion - Correct Answers -False
A special microtome able to produce very thin sections must be used for tissue samples
to be used with the electron microscope for the following reason: - Correct Answers -To
permit the passage of electrons through the tissue section
An ultramicrotome can produce sections with a thickness of a 30-40 nm which is the
thickness needed for electron microscopy - Correct Answers -True
Choose the best answer which fits how a phase contrast microscope works - Correct
Answers -Utilizes small differences in the refractive index to visualize different cell parts
Light microscope sections must be thin for the following reason: - Correct Answers -To
more easily permit light to pass through the section
,Histology is primarily the study of the structure of cells, tissues, and organs and the
relationship of microscopic structure and function. - Correct Answers -True
In simple optics of a convex lens, an imaginary image is one that can be projected onto
a screen and viewed with our own eyes. - Correct Answers -False
In a compound microscope, the objective lens produces an image that falls outside the
focal point of the ocular lens. - Correct Answers -False
In an electron microscope, the glass lens must be of a special construction to permit the
electrons to pass through. - Correct Answers -False
If the object size is 1 microns, calculate the magnification produced using a 50x
objective lens and 10x ocular lens - Correct Answers -500 microns
In the paraffin technique for producing sections for microscopic study, which statement
describes the process of dehydration, clearing and embedding. - Correct Answers -The
fixed tissue is placed in successively stronger ethyl alcohol concentrations, xylene and
then paraffin
If you would like to produce a three dimensional image of a biological specimen, which
type of microscope listed would you use? - Correct Answers -Confocal scanning
microscope
Staining of a tissue section is necessary to provide contrast to cellular components to
make them visible in the light microscope. - Correct Answers -True
Tissues are composed solely of particular types of cells - Correct Answers -False
Our brains invert the image on our retina so that we can see the world right-side up -
Correct Answers -True
One Angstrom is equivalent to: - Correct Answers -None of the listed choices
Tissue structures absorbing basic stains are termed basophobic - Correct Answers -
False
One reason for chemical fixation of a tissue section is to curtail release from dead cells
of enzymes capable of digesting tissue constituents. - Correct Answers -True
What type of microscope can be used to quantitatively measure DNA and RNA in cells -
Correct Answers -Ultraviolet microscope
Which type of microscope can be used to study the surface topography at molecular
and atomic resolution? - Correct Answers -Atomic force microscope
, When applied to histologic sections, the term ___ means a flaw caused by faulty
technique - Correct Answers -Artifact
Brown fat mitochondria from bats do not have elementary particles and this helps them
to: - Correct Answers -Produce more heat to speed up the process of getting their body
temperature back up to normal at end of hibernation
An amphipathic molecule possesses a hydrophobic end but no portion of the molecule
is hydrophilic - Correct Answers -False
Microfilaments help cells change shape, exhibit motility and divide - Correct Answers -
True
What wavelength is used in the equation for calculating resolving power or resolution? -
Correct Answers -540 nm
Which type of microscopy is useful in examining urine for crystals of uric acid and
oxalate as well as observing for the presence of spirochetes such as Treponema
pallidum, the organism causing syphilis - Correct Answers -Dark field microscope
When an object is located inside the focal point of the ocular lens of a compound
microscope, choose the statement below that is true. - Correct Answers -The image is
magnified, erect, and imaginary
When an image is located outside the focal point of the objective lens of a compound
microscope, choose the statement below that is true. - Correct Answers -The image is
magnified, inverted and classified as a real image
What is the numerical aperture of a lens? - Correct Answers -The ability of a lens to
gather light
When slides are made from frozen tissues, the sections are cut inside a refrigerated
cabinet called a ___ - Correct Answers -Cryostat
The type of microscope which is able to see the three-dimensional distribution of a
specific constituent of a living tissue by the use of a narrow laser beam that can be
precisely focused to the appropriate depth is called a ___________________. - Correct
Answers -Confocal microscope
The stage in the paraffin technique for producing a tissue slide for viewing with a light
microscope that entails using a microtome to cut the paraffin embedded tissue into thin
slices is called _____________. - Correct Answers -Sectioning
The fluid content of the gelatinous interstitial matrix surrounding small blood vessels
which enables oxygen and nutrients to diffuse from the blood fluid inside capillaries to
