HISTOLOGY & CYTOLOGY REVIEW
FOR FINAL EXAM QUESTIONS AND
ANSWERS
lysis or dissolving of cells by enzymatic action - Correct Answers -Autolysis
Breakdown of tissue by bacterial action - Correct Answers -Putrefaction
fixative classified as aldehyde - Correct Answers -Formalin
found in Bouin's fluid, is explosive and poisonous - Correct Answers -Picric Acid
good fixative for nuclei & gives great detail - Correct Answers -Zenker's Fluid
recommended for fixation of tissues for electron microscopy - Correct Answers -
Gluteraldehyde
cytology fixative - Correct Answers -95% ethanol
fixative classified as oxidizing agent - Correct Answers -Potassium Permanganate
Neutralizing agent - Correct Answers -5 % Lithium
Decalcifying agent - Correct Answers -Van Ebner Fluid
Pap stained & tested in cytology - Correct Answers -Pap Smear
uses centrifugal force to apply cells to a slide, used for CSF & Urine - Correct Answers -
Cytocentrifugation
fluid used to fix tissues and cells in their normal state - Correct Answers -Fixative
removal of overstraining or regressive staining - Correct Answers -Differentiation
study of disease - Correct Answers -Pathology
Study of cells - Correct Answers -Cytology
Study of tissues - Correct Answers -Histology
, microtome in freezer, temp of -20 to -30 C Used of rapid diagnosis, located in room
beside OR - Correct Answers -Cryostat
Used to cut specimen in wax - Correct Answers -Microtome
Parrafin wax and plastic polymers - Correct Answers -Paraplast
Looking for cancer cells - Correct Answers -Non Gyn
Specimen transferred directly to slide with no additives ie. sputum - Correct Answers -
Direct Smear
Removal of calcium salts - Correct Answers -Decalcification
infiltrating with wax - Correct Answers -Impregnation
Removal of dehydrating agents by using xylene - Correct Answers -Clearing
making wax block - Correct Answers -Embedding
cutting wax block - Correct Answers -Cutting
pap test - Correct Answers -Gyn
total removal of water by ascending grades of alcohol - Correct Answers -Dehydration
Bone marrow preparations are usually stained with this - Correct Answers -Wright's &
Romanowsky
Ripening agent of the Harris Hematoxylin stain - Correct Answers -Mercuric Oxyde
1. Fixation
2. Decalcification
3. Dehydration
4. Clearing
5. Impregnation
6. Embedding
7. Cutting
8. Staining - Correct Answers -Order to process a bone specimen
Used to pick up tissues from the water bath - Correct Answers -Charged Slide
H & E or Methylene blue - Correct Answers -What stain (s) are used for frozen
sections?
FOR FINAL EXAM QUESTIONS AND
ANSWERS
lysis or dissolving of cells by enzymatic action - Correct Answers -Autolysis
Breakdown of tissue by bacterial action - Correct Answers -Putrefaction
fixative classified as aldehyde - Correct Answers -Formalin
found in Bouin's fluid, is explosive and poisonous - Correct Answers -Picric Acid
good fixative for nuclei & gives great detail - Correct Answers -Zenker's Fluid
recommended for fixation of tissues for electron microscopy - Correct Answers -
Gluteraldehyde
cytology fixative - Correct Answers -95% ethanol
fixative classified as oxidizing agent - Correct Answers -Potassium Permanganate
Neutralizing agent - Correct Answers -5 % Lithium
Decalcifying agent - Correct Answers -Van Ebner Fluid
Pap stained & tested in cytology - Correct Answers -Pap Smear
uses centrifugal force to apply cells to a slide, used for CSF & Urine - Correct Answers -
Cytocentrifugation
fluid used to fix tissues and cells in their normal state - Correct Answers -Fixative
removal of overstraining or regressive staining - Correct Answers -Differentiation
study of disease - Correct Answers -Pathology
Study of cells - Correct Answers -Cytology
Study of tissues - Correct Answers -Histology
, microtome in freezer, temp of -20 to -30 C Used of rapid diagnosis, located in room
beside OR - Correct Answers -Cryostat
Used to cut specimen in wax - Correct Answers -Microtome
Parrafin wax and plastic polymers - Correct Answers -Paraplast
Looking for cancer cells - Correct Answers -Non Gyn
Specimen transferred directly to slide with no additives ie. sputum - Correct Answers -
Direct Smear
Removal of calcium salts - Correct Answers -Decalcification
infiltrating with wax - Correct Answers -Impregnation
Removal of dehydrating agents by using xylene - Correct Answers -Clearing
making wax block - Correct Answers -Embedding
cutting wax block - Correct Answers -Cutting
pap test - Correct Answers -Gyn
total removal of water by ascending grades of alcohol - Correct Answers -Dehydration
Bone marrow preparations are usually stained with this - Correct Answers -Wright's &
Romanowsky
Ripening agent of the Harris Hematoxylin stain - Correct Answers -Mercuric Oxyde
1. Fixation
2. Decalcification
3. Dehydration
4. Clearing
5. Impregnation
6. Embedding
7. Cutting
8. Staining - Correct Answers -Order to process a bone specimen
Used to pick up tissues from the water bath - Correct Answers -Charged Slide
H & E or Methylene blue - Correct Answers -What stain (s) are used for frozen
sections?