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Genetics & Evolution Exam 4 : Recombinant DNA Engineering, Transgenic Bacteria & Plants, PCR Amplification, STR Profiling, Stem Cells (Totipotent & Pluripotent), Cloning, Somatic Cell Nuclear Transfer, DNA Probes, Mutation, Natural & Artificial Selection,

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Genetics & Evolution Exam 4 : Recombinant DNA Engineering, Transgenic Bacteria & Plants, PCR Amplification, STR Profiling, Stem Cells (Totipotent & Pluripotent), Cloning, Somatic Cell Nuclear Transfer, DNA Probes, Mutation, Natural & Artificial Selection, Directional/Disruptive/Stabilizing Selection, Balanced Polymorphism, Sexual Dimorphism, Genetic Drift, Founder & Bottleneck Effects, Gene Flow, Nonrandom Mating, Fossil Records, Relative/Absolute/Radiometric Dating, Biogeography, Plate Tectonics, Homologous & Analogous Structures, Convergent Evolution, Vestigial Traits, Speciation (Allopatric & Sympatric), Polyploidy, Adaptive Radiation, Extinction, Phylogenetics & Cladograms, Taxonomic Classification, Reproductive Isolation (Prezygotic & Postzygotic) Exam Questions Verified and Provided with Complete A+ Graded Rationales Latest Updated 2026 Recombinant DNA genetic material joined together from multiple sources (typically different species) transgenic organism organism containing DNA from multiple species Transgenic bacteria Uptake recombinant DNA Transgenic Plants Recombinant DNA from a transgenic bacteria that infect the plant's cells Transgenic Animals Recombinant DNA from viruses that infect gamete or fertilized egg of animal DNA sequencing the process of determining the precise order of the nucleotides within a DNA molecule Purpose of PCR Produces millions of copies of a selected DNA sequence. Used by researchers to make enough DNA for experiments, genetic testing, etc. Components needed for PCR Nucleotides, Primers, Target DNA, DNA Polymerase DNA Profiling uses the most variable parts of the genome to detect genetic differences between individuals (ex: can use STR's) short tandem repeats (STRs) Sequences of a few nucleotides that are repeated in noncoding regions of DNA Why are STRs more useful than whole genome sequencing? It uses regions that are highly variable so can detect genetic differences using a lot less information and thus is cheaper. Genome sequencing is very expensive and produces a massive amount of information since is sequences all the DNA Applications of STR in genetic profiling Can be used in forensic testing to determine who committed a crime, if it is a missing person, parentage testing, etc. If given a sample of DNA from a crime scene and DNA from suspects, look for a match to STR data between crime scene DNA and suspects. Ex: Both have 5 STR repeats Stem Cells

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Genetics & Evolution Exam 4 : Recombinant DNA Engineering, Transgenic
Bacteria & Plants, PCR Amplification, STR Profiling, Stem Cells (Totipotent &
Pluripotent), Cloning, Somatic Cell Nuclear Transfer, DNA Probes, Mutation,
Natural & Artificial Selection, Directional/Disruptive/Stabilizing Selection,
Balanced Polymorphism, Sexual Dimorphism, Genetic Drift, Founder &
Bottleneck Effects, Gene Flow, Nonrandom Mating, Fossil Records,
Relative/Absolute/Radiometric Dating, Biogeography, Plate Tectonics,
Homologous & Analogous Structures, Convergent Evolution, Vestigial Traits,
Speciation (Allopatric & Sympatric), Polyploidy, Adaptive Radiation, Extinction,
Phylogenetics & Cladograms, Taxonomic Classification, Reproductive Isolation
(Prezygotic & Postzygotic) Exam Questions Verified and Provided with Complete
A+ Graded Rationales Latest Updated 2026




Recombinant DNA

genetic material joined together from multiple sources (typically different species)




transgenic organism

organism containing DNA from multiple species

,Transgenic bacteria

Uptake recombinant DNA




Transgenic Plants

Recombinant DNA from a transgenic bacteria that infect the plant's cells




Transgenic Animals

Recombinant DNA from viruses that infect gamete or fertilized egg of animal




DNA sequencing

the process of determining the precise order of the nucleotides within a DNA molecule




Purpose of PCR

Produces millions of copies of a selected DNA sequence. Used by researchers to make enough
DNA for experiments, genetic testing, etc.




Components needed for PCR

, Nucleotides, Primers, Target DNA, DNA Polymerase




DNA Profiling

uses the most variable parts of the genome to detect genetic differences between individuals
(ex: can use STR's)




short tandem repeats (STRs)

Sequences of a few nucleotides that are repeated in noncoding regions of DNA




Why are STRs more useful than whole genome sequencing?

It uses regions that are highly variable so can detect genetic differences using a lot less
information and thus is cheaper. Genome sequencing is very expensive and produces a massive
amount of information since is sequences all the DNA




Applications of STR in genetic profiling

Can be used in forensic testing to determine who committed a crime, if it is a missing person,
parentage testing, etc. If given a sample of DNA from a crime scene and DNA from suspects,
look for a match to STR data between crime scene DNA and suspects. Ex: Both have 5 STR
repeats




Stem Cells

undifferentiated cell that divides to give rise to additional stem cells and cells that specialize

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