Transfusion CSMLS with correct answers 2026

Transfusion CSMLS with correct answers 2026 Kleihauer Betke Test - Correct Answer If Rosette test is positive, quantifies FMH. Acid elution principle, fetal hemoglobin (HgbF) is resistant to acid elution, adult hemoglobin (HgbA) is not resistant. -Quantitative test 1. Blood smear from postpartum maternal sample exposed to acid buffer. 2. HgB from adult red cells leaches into buffer and leaves only stroma, whereas the fetal red cells retain their Hgb 3. Smears washed, stained and examined under oil immersion. 4. Adult red cells appear as "ghosts", fetal cells appear pink Reported as percentage of fetal red cells ( # fetal red cells / total red cell count) #fetal cells/1000 RBC Rosette Test - Correct Answer Screen method , demonstrates presence of Rh Positive Baby cells. - Qualitative test Suspension of maternal Red cells (contains D -negative maternal and small # of D-positive fetal red cells) incubated with Anti-D. Anti-D binds to D-pos fetal cells. Suspension then washed and D positive indicator red cells added which bind to anti-D and form rosette around D positive fetal red cells, which is then examined microscopically. 1 rosette per 3 lpf: 1 dose of RhIG 1 rosette per 3pg: quantitate bleed ACD, CPD, CP2D - Correct Answer 21 days after used (Anticoagulants) CPDA-1 - Correct Answer Anticoagulant 35 day expiration Additive Solutions - Correct Answer Preserving solutions that are added to the RBCs after removal of the plasma with or without platelets 42 days Packed Red Blood Cells (PRBCs) - Correct Answer Red cells with plasma removed To increase oxygen-carrying capacity in patients with anemia, in patients with substantial hemoglobin deficits, and in patients who have lost up to 25% of their total blood volume Washed Red Cells - Correct Answer - Plasma removed by successive saline washes (automated instruments) - Prevents allergic response to plasma proteins and anaphylactic shock in IgA deficient patients with anti-IgA (IgA is in normal plasma) - Expires 24 hours after seal of original unit broken Leukocyte reduced RBCs - Correct Answer Removal of leukocytes (WBCs) from the RBC component resulting in 5 x 10^6 WBCs in the entire unit. 85% of RBCs retained. - For patients with repeated febrile nonhemolytic FNH reactions. Deglycerolized RBCs - Correct Answer Cryoprotective agent (40% glycerol) protect from ultra low temperatures. - Thawed @ 37c and glycerol removed. 80% of RBCs must be recovered. - Stored @ -65c; 1-6c for 24hrs after deglycerolizing - Used for storage of autologous units and rare units; expires in 10 years. Fresh Frozen Plasma (FFP) - Correct Answer -Prepared by separating cells and plasma by centrifugation and freezing plasma within 8 hours of collection -Expires 1 year from date of collection when stored at ≤ 18°C or 7 years stored at ≤ -65°C -Once thawed (between 30-37°C), expires in 24 hours, if stored at 1-6°C - Used for multiple coagulation deficiencies, factor XI deficiency and other congenital deficiencies for which no concentrate is available Cryoprecipitate - Correct Answer FFP frozen within 8 hrs of WB collection is thawed @ 1-6C, cold insoluble portion of plasma forms. It is refrozen within 1 hour. - Contains 150mg fibrinogen and 80IU/bag of factor VIII, Contains vWF, factor XIII and fibronectin - Stored @ -18c for 1 year from date of draw, room temp after thawing. Transfuse within 6 hrs - Used for : Fib and FXIII deficiencies, severe VWD, topical fibrin sealant. Factor Concentrates - Correct Answer Concentrates prepared from plasma pools that are nested by NAT and processed to inactive/remove pathogens. Inc level of specific factors. Hemo A : Factor VIII