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Microbiology 251 Final Learning Objectives TEST FINAL EXAM
AND PRACTICE EXAM 2026/2027 BANK 2 VERSIONS
QUESTIONS WITH DETAILED VERIFIED ANSWERS / EXAM
QUESTIONS WILL COME FROM HERE (100% CORRECT
ANSWERS/ A+ GRADED
If E. coli expresses the pGLO plasmid, we can expect all of the following
except:
1. E. coli will glow green if arabinose is present in the environment
2. E. coli will be vancomycin resistant
3. E. coli shows resistance against beta-lactam antibiotics
4. E. coli can grow in the presence of ampicillin - ANSWERS--E. coli will
be vancomycin resistant
A Simmons citrate slant turns bright blue. All of the following
statements would be true except:
1. The Simmons citrate test is a selective test
2. The bacterium can transport citrate into its cytoplasm
3. The bacterium expresses the enzyme citrate permease
4. The Simmons citrate test is a differential test - ANSWERS--The
Simmons citrate test is a differential test
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To determine the zone of inhibition formed by a bacterial species
against the antibiotic, measure the:
1. Radius of the zone of inhibition in millimeters
2. Radius of the zone of inhibition in centimeters
3. Diameter of the zone of inhibition in centimeters
4. Diameter of the zone of inhibition in millimeters - ANSWERS--
Diameter of the zone of inhibition in millimeters
Areas of clearing or holes in the bacterial lawn caused by phage lysis
are known as:
1. Zones of destruction
2. Formites
3. Colony forming units
4. Plaques - ANSWERS--Plaques
A nurse swabs a patients skin with alcohol before inserting an IV drip
into the patients vein. Alcohol would best be described as a(n):
1. Disinfectant
2. Antibiotic
3. Bacteriostatic agent
4. Antiseptic - ANSWERS--Antiseptic
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In a thermal death point assay, the temperature is __________ and the
time is _________.
1. Variable; constant
2. Constant; variable
3. Constant; constant
4. Variable; variable - ANSWERS--Variable; constant
A bactericidal agent would be expected to:
1. Kill the bacterium
2. Inhibit the growth of the bacterium - ANSWERS--Kill the bacterium
You streak a streptococcal species on a blood agar plate and see alpha-
hemolytic activity. What can you conclude?
1. You can conclude you most likely have a pathogenic streptococcal
species
2. You can conclude you most likely have a non-pathogenic
streptococcal species - ANSWERS--You can conclude you most likely
have a non-pathogenic streptococcal species
Which of the following represents our susceptible host in the
simulation of a sneeze exercise?
1. Bacillus subtilis var. globigii
2. E. Coli B strain
3. Bacteriophage T2
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4. E. Coli asmB1 strain - ANSWERS--E. Coli B strain
A researcher tests mucous secretions for antimicrobial activity and
observes that the mucous is effective in inhibiting bacterial growth.
Which of the following chemicals night be present in the mucous and
responsible for the antibacterial action observed?
1. Superoxide dismutase
2. Lysozyme
3. Catalase
4. Lipase - ANSWERS--Lysozyme
In the fluid thioglycollate tube indicated by the blue arrow, how would
you describe the organisms oxygen requirements based on its growth
pattern? (Pic in pics)
1. Microaerophile
2. Facultative
3. Obligate aerobe
4. Aerotolerant
5. Obligate anaerobe - ANSWERS--Obligate anaerobe
The lethal and mutagenic effects of UV radiation on bacterial cells are
primarily due to damage to:
1. DNA
2. RNA
Microbiology 251 Final Learning Objectives TEST FINAL EXAM
AND PRACTICE EXAM 2026/2027 BANK 2 VERSIONS
QUESTIONS WITH DETAILED VERIFIED ANSWERS / EXAM
QUESTIONS WILL COME FROM HERE (100% CORRECT
ANSWERS/ A+ GRADED
If E. coli expresses the pGLO plasmid, we can expect all of the following
except:
1. E. coli will glow green if arabinose is present in the environment
2. E. coli will be vancomycin resistant
3. E. coli shows resistance against beta-lactam antibiotics
4. E. coli can grow in the presence of ampicillin - ANSWERS--E. coli will
be vancomycin resistant
A Simmons citrate slant turns bright blue. All of the following
statements would be true except:
1. The Simmons citrate test is a selective test
2. The bacterium can transport citrate into its cytoplasm
3. The bacterium expresses the enzyme citrate permease
4. The Simmons citrate test is a differential test - ANSWERS--The
Simmons citrate test is a differential test
,2|Page
To determine the zone of inhibition formed by a bacterial species
against the antibiotic, measure the:
1. Radius of the zone of inhibition in millimeters
2. Radius of the zone of inhibition in centimeters
3. Diameter of the zone of inhibition in centimeters
4. Diameter of the zone of inhibition in millimeters - ANSWERS--
Diameter of the zone of inhibition in millimeters
Areas of clearing or holes in the bacterial lawn caused by phage lysis
are known as:
1. Zones of destruction
2. Formites
3. Colony forming units
4. Plaques - ANSWERS--Plaques
A nurse swabs a patients skin with alcohol before inserting an IV drip
into the patients vein. Alcohol would best be described as a(n):
1. Disinfectant
2. Antibiotic
3. Bacteriostatic agent
4. Antiseptic - ANSWERS--Antiseptic
,3|Page
In a thermal death point assay, the temperature is __________ and the
time is _________.
1. Variable; constant
2. Constant; variable
3. Constant; constant
4. Variable; variable - ANSWERS--Variable; constant
A bactericidal agent would be expected to:
1. Kill the bacterium
2. Inhibit the growth of the bacterium - ANSWERS--Kill the bacterium
You streak a streptococcal species on a blood agar plate and see alpha-
hemolytic activity. What can you conclude?
1. You can conclude you most likely have a pathogenic streptococcal
species
2. You can conclude you most likely have a non-pathogenic
streptococcal species - ANSWERS--You can conclude you most likely
have a non-pathogenic streptococcal species
Which of the following represents our susceptible host in the
simulation of a sneeze exercise?
1. Bacillus subtilis var. globigii
2. E. Coli B strain
3. Bacteriophage T2
, 4|Page
4. E. Coli asmB1 strain - ANSWERS--E. Coli B strain
A researcher tests mucous secretions for antimicrobial activity and
observes that the mucous is effective in inhibiting bacterial growth.
Which of the following chemicals night be present in the mucous and
responsible for the antibacterial action observed?
1. Superoxide dismutase
2. Lysozyme
3. Catalase
4. Lipase - ANSWERS--Lysozyme
In the fluid thioglycollate tube indicated by the blue arrow, how would
you describe the organisms oxygen requirements based on its growth
pattern? (Pic in pics)
1. Microaerophile
2. Facultative
3. Obligate aerobe
4. Aerotolerant
5. Obligate anaerobe - ANSWERS--Obligate anaerobe
The lethal and mutagenic effects of UV radiation on bacterial cells are
primarily due to damage to:
1. DNA
2. RNA
