FIS 201 – Final Exam Study Guide (WVU, Mohr)
Marquis Reagent - ANS✔✔ Turns purple with Heroin and Morphine, orange/brown with
amphetamines
Dillie-Koppanyi - ANS✔✔ Violet blue with Barbiturates
Duquenosis-Levine - ANS✔✔ Purple with marijuana when chloroform is added
Van Urk - ANS✔✔ Blue/purple with LSD (not appropriate for field tests)
Scott Test - ANS✔✔ Color test for Cocaine. Adding cobalt thiocyanate turns solution blue, add
hydrochloric acid and blue turns to pink, then add chloroform and it turns back to blue for
positive result.
Chromatography - ANS✔✔ Any of several analytical techniques for separating organic mixtures
into their components by attraction to their stationary phase by a mobile phase
Stationary phase - ANS✔✔ substance that stays fixed
Mobile phase - ANS✔✔ solvent used to separate the mixture
Thin Layer Chrom. (TLC) - ANS✔✔ Uses a solid stationary phase and a mobile liquid phase to
separate inks or dyes. Very rapid and sensitive.
Gas Chrom. (GC) - ANS✔✔ Separates mixtures based on their distribution between a stationary
liquid phase and a moving gas phase. As the gas flows through the columns it carries the
components of the mixture. (Cannot produce specific identification)
, Spectrophotometry - ANS✔✔ Identifies a substance by its selective absorption to different
wavelengths of light (non destructive)
Mass Spectro. (MS) - ANS✔✔ Material from the GC enters MS in a chamber where a beam of
electrons collide with the molecules. MS provides the specifics that GC doesn't.
Scanning Electron Microscope (SEM) - ANS✔✔ Type of microscope that produces images of a
sample by scanning it with a focused beam of electrons.
Comparison Microscope - ANS✔✔ Used to analyze side by side specimen up close
ELISA test (immunoassay) - ANS✔✔ Test that detects and measures antibodies in a persons
blood to determine if you have antibodies related to certain infectious conditions or diseases.
Short Tandem Repeats (STR) - ANS✔✔ DNA regions with short repeated strands that contain
polymerase chain reactions (PCR) which help in identification of a person.
Questioned Document - ANS✔✔ Any document whose authenticity or origin is suspect either as
a whole or a part.
Albert S. Osborn - ANS✔✔ Teacher of penmanship, encouraged courts to accept questioned
documents as evidence, founded ASQDE
Professional groups for questioned documents - ANS✔✔ ASQDE, NADE, ABFDE
Questioned document Examiner tasks - ANS✔✔ Establish forgery, Identify or eliminate
handwriting or source of typewriting
Marquis Reagent - ANS✔✔ Turns purple with Heroin and Morphine, orange/brown with
amphetamines
Dillie-Koppanyi - ANS✔✔ Violet blue with Barbiturates
Duquenosis-Levine - ANS✔✔ Purple with marijuana when chloroform is added
Van Urk - ANS✔✔ Blue/purple with LSD (not appropriate for field tests)
Scott Test - ANS✔✔ Color test for Cocaine. Adding cobalt thiocyanate turns solution blue, add
hydrochloric acid and blue turns to pink, then add chloroform and it turns back to blue for
positive result.
Chromatography - ANS✔✔ Any of several analytical techniques for separating organic mixtures
into their components by attraction to their stationary phase by a mobile phase
Stationary phase - ANS✔✔ substance that stays fixed
Mobile phase - ANS✔✔ solvent used to separate the mixture
Thin Layer Chrom. (TLC) - ANS✔✔ Uses a solid stationary phase and a mobile liquid phase to
separate inks or dyes. Very rapid and sensitive.
Gas Chrom. (GC) - ANS✔✔ Separates mixtures based on their distribution between a stationary
liquid phase and a moving gas phase. As the gas flows through the columns it carries the
components of the mixture. (Cannot produce specific identification)
, Spectrophotometry - ANS✔✔ Identifies a substance by its selective absorption to different
wavelengths of light (non destructive)
Mass Spectro. (MS) - ANS✔✔ Material from the GC enters MS in a chamber where a beam of
electrons collide with the molecules. MS provides the specifics that GC doesn't.
Scanning Electron Microscope (SEM) - ANS✔✔ Type of microscope that produces images of a
sample by scanning it with a focused beam of electrons.
Comparison Microscope - ANS✔✔ Used to analyze side by side specimen up close
ELISA test (immunoassay) - ANS✔✔ Test that detects and measures antibodies in a persons
blood to determine if you have antibodies related to certain infectious conditions or diseases.
Short Tandem Repeats (STR) - ANS✔✔ DNA regions with short repeated strands that contain
polymerase chain reactions (PCR) which help in identification of a person.
Questioned Document - ANS✔✔ Any document whose authenticity or origin is suspect either as
a whole or a part.
Albert S. Osborn - ANS✔✔ Teacher of penmanship, encouraged courts to accept questioned
documents as evidence, founded ASQDE
Professional groups for questioned documents - ANS✔✔ ASQDE, NADE, ABFDE
Questioned document Examiner tasks - ANS✔✔ Establish forgery, Identify or eliminate
handwriting or source of typewriting
