SBB PRACTICE SCRIPT UPDATED 2026
TESTED SOLUTIONS
⫸ KEL3 Issues Answer: KPa
in cis position may have suppressive effect on other Kell system
antigens
⫸ Adsorption applications in RBC testing Answer: Remove
autoantibody
Separate multiple antibodies
Confirm antigen or antibody specificity
⫸ Antilymphocyte Globulin (ALG) Answer: Made from horse serum
Interferes with DAT and IAT
Relation to Lu(a-b-)
⫸ PCR and TMA Answer: PCR used for DNA amp
TMA used for RNA amp
⫸ Autologous donor qualifications Answer: DR's order
Hgb >11, HCT 33%
Collected >72 hrs before surgery or Tx
,Deferred if risk of bacteremia
⫸ Plateletpheresis count info Answer: >150,000 min
pre procedure plt count not required for infrequent donors
post-procedure plt count may be used to qualify donor for next
procedure
⫸ Two units RBC apheresis post donation H/H Answer: Donor HCT
not <30% or Hgb <10 g/dL after volume replacement following
donation
⫸ Anticoagulants and purpose of Answer: Dextrose:
Supports ATP generation
Adenine:
Provides substrate for ATP synthesis
Sodium biphosphate:
Controls pH or is buffer
Citrate:
prevents clotting and coagulation
⫸ Changes in bag upon storage Answer: decreases in:
pH
2,3-DPG
ATP
, Increases in:
Hgb
K
⫸ Oxygen dissociation curve information Answer: p50 is pO2 level at
which Hgb is 50% saturated
A normal p50 is about 25 pO2
increase p50, see right shift, more O2 released
decrease p50, see left shift, less O2 released
⫸ Reactive test results for TTD action Answer: Reactive results must be
retested in duplicate
⫸ Confirmatory TTD tests Answer: Neutralization for HBsAg and HIV-
1 Ag
WB for Anti-HIV
RIBA for Anti-HCV
Anti-HTLV confirmation
⫸ No algorithm available for reentry by FDA for these Answer: HIV
and HCV NAT pos tests
⫸ Components for correction of coagulopathies Answer: Plasma
PLTs
TESTED SOLUTIONS
⫸ KEL3 Issues Answer: KPa
in cis position may have suppressive effect on other Kell system
antigens
⫸ Adsorption applications in RBC testing Answer: Remove
autoantibody
Separate multiple antibodies
Confirm antigen or antibody specificity
⫸ Antilymphocyte Globulin (ALG) Answer: Made from horse serum
Interferes with DAT and IAT
Relation to Lu(a-b-)
⫸ PCR and TMA Answer: PCR used for DNA amp
TMA used for RNA amp
⫸ Autologous donor qualifications Answer: DR's order
Hgb >11, HCT 33%
Collected >72 hrs before surgery or Tx
,Deferred if risk of bacteremia
⫸ Plateletpheresis count info Answer: >150,000 min
pre procedure plt count not required for infrequent donors
post-procedure plt count may be used to qualify donor for next
procedure
⫸ Two units RBC apheresis post donation H/H Answer: Donor HCT
not <30% or Hgb <10 g/dL after volume replacement following
donation
⫸ Anticoagulants and purpose of Answer: Dextrose:
Supports ATP generation
Adenine:
Provides substrate for ATP synthesis
Sodium biphosphate:
Controls pH or is buffer
Citrate:
prevents clotting and coagulation
⫸ Changes in bag upon storage Answer: decreases in:
pH
2,3-DPG
ATP
, Increases in:
Hgb
K
⫸ Oxygen dissociation curve information Answer: p50 is pO2 level at
which Hgb is 50% saturated
A normal p50 is about 25 pO2
increase p50, see right shift, more O2 released
decrease p50, see left shift, less O2 released
⫸ Reactive test results for TTD action Answer: Reactive results must be
retested in duplicate
⫸ Confirmatory TTD tests Answer: Neutralization for HBsAg and HIV-
1 Ag
WB for Anti-HIV
RIBA for Anti-HCV
Anti-HTLV confirmation
⫸ No algorithm available for reentry by FDA for these Answer: HIV
and HCV NAT pos tests
⫸ Components for correction of coagulopathies Answer: Plasma
PLTs
