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Where does ACh act on? -answer Somatic nervous system: skeletal muscle
Parasympathetic division: Cardiac muscle
Sympathetic division: preganglionic nerves in smooth muscle and glands
What is choline acetyltransferase? -answer Synthesizes ACh from choline and acetyl
CoA
What does Acetylcholinesterase do? -answer Degrades Acetylcholine into choline and
acetate
describe Ach synthesis and degradation -answer 1. Choline is transported into the
presynaptic cholinergic nerve terminal by Na+ choline cotransporter
2. Choline acetyltransferase catalyses the formation of ACh from acetyl CoA and choline
3. Newly synthesized ACh is packaged into vesicles for storage
4. Nerve stimulation = ACh release by exocytosis
, 5. Released Ach diffuses in the synaptic cleft - binds to postsynaptic and presynaptic receptors
6. ACh in the synaptic cleft is degraded by membrane-bound acetylcholinesterase (AChE)
What drug inhibits choline uptake? -answer Hemicholinium
What drug inhibits ACh storage? -answer vesamicol
What drug inhibits ACh release? -answer Botulinum toxin
What drug inhibits acetylcholinesterase? -answer Nerve gases
What drug stimulates ACh release into cleft? -answer a-latrotoxin
how does botulinum toxin work? -answer Blocks ACh release into the cleft
Inhibits neuromuscular transmission
Relaxes both skeletal and smooth muscle
Adverse effects: Dry mouth, difficulty swallowing
How does a latrotoxin work? -answer Stimulates ACh release into the cleft
Excessive activation of ACh receptors