SBB QUESTIONS AND ANSWERS
WITH CORRECT SOLUTIONS
UPDATED
Donors must be given educational material regarding: Infection diseases transmitted by
blood transfusion
Signs and symptoms of AIDS
Risks of procedure
Circumstances in which infection disease testing is not performed
should be given chance to ask questions
Donor qualifications: pay particular attention to the following: Donation interval
vitals
HGB/HCT
receipt of blood or other human tissue
Immunizations
travel
infectious diseases
Leukapheresis donors may be given what drugs to enhance collections? Hydroxyethyl starch
Corticosteroids
Growth factors
RIBA - Anti-HCV
Anti-HTLV confirmed by repeating the test using another manufacturers EIA test
HTLV testing and deferral Two+ positives: 2 donations by same manufacturer = indefinite
deferral
1+ by one manuf. and 1+ by another manuf = indef.defer.
1+ by one manuf. and negative by another manuf. = 8 week deferral
What does Dextrose in additive do for cells? supports ATP generation
What does Adenine in additive do for cells? provides substrate for ATP synthesis
What does sodium biphosphate in additive do for cells? controls pH
What does citrate in additive do for cells? prevents coagulation
What is the storage time if additive used is CPD, CP2D? 21 days
What is the storage time if additive used is CPDA-1? 35 days
,Identifying information on the donor form should include: Date and time of donation
Name
Address
Telephone #
Gender
Age or DOB
A record of reasons for prior deferrals, if any
What is the storage time if additive used is AS-1 (Adsol)? 42 days
What is the storage time if additive used is AS-3 (nutricel)? 42 days
What is the storage time if additive used is AS-5 (Optisol)? 42 days
Whole Blood storage temp and description 1-6C
contains all blood elements plus the anticoagulant-preservative commonly used as a source for
component production
RBC storage and description 1-6C
Whole blood with plasma removed
RBCs without additives must have final hct <80%
Frozen RBC storage and description -65C or colder
frozen within 6 days if no additive solution
RBC w/additive must be frozen before expiration
must be put in freezer within 4 hours of removed from 4C
Rejuvenated RBC descriptionrejuvenation should restore 2,3-DPG to 75% of normal level and
ATP above normal levels.
If done before 14 days may cause high 2,3-DPG which impairs O2 up-take.
AS-3 and AS-5 cannot be rejuvenated
Deglyced RBCs storage and description 37C warmer or waterbath (20-25 minutes)
washed with solutions of decreasing osmolarity to avoid hemolysis.
must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least 80%
RBC volume.
open system:1-6C with 24 hr expiration.
closed system: 14 days or as FDA approved
What should the osmolality of a deglyced unit be? <500.
Osmolality is a fxn of free glycerol therefore an increase is a result of improper deglycing
What should free hgb in last wash be from decglyed unit? <200
, Washed RBCs storage1-6C
washed with sterile normal saline to remove most of plasma
24 hour expiration
FFP storage and description prepared from WB or collected using apheresis.
frozen at -18C or below
contains max levels of labile or nonlabile coag factors
Thaw between 30-37C
Thawed plasma FFP thawed but not transfused within 24 hrs
can be stored 1-6C for 5 days
reduction in Factors V and VII
Liquid plasma plasma separated from WB at any time during storage, up to 5 days after the
expiration date of WB. supernatant levels of potassium and ammonia are increased
Cryo prepared from WB collected into CPDA-1 or CPD
precipitates when FFP thawed between 1-6C
stored at -18C or colder
suspended in less than 15mL plasma
contains factor VII and fibrinogen and most of XIII
single unit store at 20-24 for 6 hrs
pooled store at 20-24 for 4 hours
platelets prepared from WB that hasn't cooled below 20C
separated within 4 hours
soft spin (plt rich plasma) followed by a hard spin (platelet concentrate)
resuspended in about 45-65mL plasma
20-24C with gentle agitation
Granulocytes usually harvested in buffy coats
collection must yield 1.0x10 to the 10th granulocytes
Transfuse ASAP
Irradiation prevents proliferation of transfused T-Lymphs, the primary cause of graft vs host
(GVHD)
effects of storage on blood components decreases 2,3-DPG levels: this controls release of
O2 from RBCs, levels recover after 24 hrs post txn
decreases ATP
increases potassium
Leukocyte reduction must reduce WBC count to what? <5x10 to the sixth in at least 95% of
units samples and yield >85% RBC recovery
WITH CORRECT SOLUTIONS
UPDATED
Donors must be given educational material regarding: Infection diseases transmitted by
blood transfusion
Signs and symptoms of AIDS
Risks of procedure
Circumstances in which infection disease testing is not performed
should be given chance to ask questions
Donor qualifications: pay particular attention to the following: Donation interval
vitals
HGB/HCT
receipt of blood or other human tissue
Immunizations
travel
infectious diseases
Leukapheresis donors may be given what drugs to enhance collections? Hydroxyethyl starch
Corticosteroids
Growth factors
RIBA - Anti-HCV
Anti-HTLV confirmed by repeating the test using another manufacturers EIA test
HTLV testing and deferral Two+ positives: 2 donations by same manufacturer = indefinite
deferral
1+ by one manuf. and 1+ by another manuf = indef.defer.
1+ by one manuf. and negative by another manuf. = 8 week deferral
What does Dextrose in additive do for cells? supports ATP generation
What does Adenine in additive do for cells? provides substrate for ATP synthesis
What does sodium biphosphate in additive do for cells? controls pH
What does citrate in additive do for cells? prevents coagulation
What is the storage time if additive used is CPD, CP2D? 21 days
What is the storage time if additive used is CPDA-1? 35 days
,Identifying information on the donor form should include: Date and time of donation
Name
Address
Telephone #
Gender
Age or DOB
A record of reasons for prior deferrals, if any
What is the storage time if additive used is AS-1 (Adsol)? 42 days
What is the storage time if additive used is AS-3 (nutricel)? 42 days
What is the storage time if additive used is AS-5 (Optisol)? 42 days
Whole Blood storage temp and description 1-6C
contains all blood elements plus the anticoagulant-preservative commonly used as a source for
component production
RBC storage and description 1-6C
Whole blood with plasma removed
RBCs without additives must have final hct <80%
Frozen RBC storage and description -65C or colder
frozen within 6 days if no additive solution
RBC w/additive must be frozen before expiration
must be put in freezer within 4 hours of removed from 4C
Rejuvenated RBC descriptionrejuvenation should restore 2,3-DPG to 75% of normal level and
ATP above normal levels.
If done before 14 days may cause high 2,3-DPG which impairs O2 up-take.
AS-3 and AS-5 cannot be rejuvenated
Deglyced RBCs storage and description 37C warmer or waterbath (20-25 minutes)
washed with solutions of decreasing osmolarity to avoid hemolysis.
must remove cryoprotectant agents, minimial free hgb, and result in recovery of at least 80%
RBC volume.
open system:1-6C with 24 hr expiration.
closed system: 14 days or as FDA approved
What should the osmolality of a deglyced unit be? <500.
Osmolality is a fxn of free glycerol therefore an increase is a result of improper deglycing
What should free hgb in last wash be from decglyed unit? <200
, Washed RBCs storage1-6C
washed with sterile normal saline to remove most of plasma
24 hour expiration
FFP storage and description prepared from WB or collected using apheresis.
frozen at -18C or below
contains max levels of labile or nonlabile coag factors
Thaw between 30-37C
Thawed plasma FFP thawed but not transfused within 24 hrs
can be stored 1-6C for 5 days
reduction in Factors V and VII
Liquid plasma plasma separated from WB at any time during storage, up to 5 days after the
expiration date of WB. supernatant levels of potassium and ammonia are increased
Cryo prepared from WB collected into CPDA-1 or CPD
precipitates when FFP thawed between 1-6C
stored at -18C or colder
suspended in less than 15mL plasma
contains factor VII and fibrinogen and most of XIII
single unit store at 20-24 for 6 hrs
pooled store at 20-24 for 4 hours
platelets prepared from WB that hasn't cooled below 20C
separated within 4 hours
soft spin (plt rich plasma) followed by a hard spin (platelet concentrate)
resuspended in about 45-65mL plasma
20-24C with gentle agitation
Granulocytes usually harvested in buffy coats
collection must yield 1.0x10 to the 10th granulocytes
Transfuse ASAP
Irradiation prevents proliferation of transfused T-Lymphs, the primary cause of graft vs host
(GVHD)
effects of storage on blood components decreases 2,3-DPG levels: this controls release of
O2 from RBCs, levels recover after 24 hrs post txn
decreases ATP
increases potassium
Leukocyte reduction must reduce WBC count to what? <5x10 to the sixth in at least 95% of
units samples and yield >85% RBC recovery
