BIO 99 #2 Questions With Complete Solutions
A polylinker is: Correct Answers A synthetic fragment that
contains multiple sites for restriction enzymes to cut the DNA.
Base excision repair is responsible for fixing (select all that
apply): Correct Answers single stranded breaks.
individual damaged nucleotides that minimally impact the helix
structure.
Different types of mutations include silent, missense and
nonsense. Match each with the descriptions below: Correct
Answers .A Results in the creation of a truncated, nonfunctional
protein.
SILENT
B. Results in the creation of a protein of normal length, but
possibly altered function.
MISSENSE
C. Results in a base substitution without any noticeable effects.
= NONSENSE
DNA Polymerase Correct Answers synthesizes new strand
DNA on the basis of the template strand,
DNA primase, DNA helicase, DNA ligase, and topoisomerase.
, Double stranded break (DSB) mechanisms for genome editing?
Select all that apply. Correct Answers Repair of breaks by
NHEJ can lead to gene inactivation.
+
The addition of foreign DNA can lead to specific gene
modifications.
double stranded breaks (DSB)? Correct Answers DSB repair is
handled by the same factors involved in homologous
recombination during meiosis
DSB repair pathways were likely essential before genome sizes
could increase in an organism
Mutations in genes involved in DSB repair can lead to cancer
double stranded breaks REPAIR by non-homologous end
joining is more likely to occur outside of S phase and G2 than
homologous recombination. Why is this? Correct Answers
Outside of S phase and G2, there is no homologous chromosome
that the homologous recombination pathway can use for repair.
Double-stranded break repair REPAIR DAMAGED
TEMPLATE ENCOUNTER Correct Answers The replication
fork encounters a lesion at which repair has been initiated and
the fork collapses.
end replication problem more protect which cells? Select all that
apply. Correct Answers germ cancer stem
A polylinker is: Correct Answers A synthetic fragment that
contains multiple sites for restriction enzymes to cut the DNA.
Base excision repair is responsible for fixing (select all that
apply): Correct Answers single stranded breaks.
individual damaged nucleotides that minimally impact the helix
structure.
Different types of mutations include silent, missense and
nonsense. Match each with the descriptions below: Correct
Answers .A Results in the creation of a truncated, nonfunctional
protein.
SILENT
B. Results in the creation of a protein of normal length, but
possibly altered function.
MISSENSE
C. Results in a base substitution without any noticeable effects.
= NONSENSE
DNA Polymerase Correct Answers synthesizes new strand
DNA on the basis of the template strand,
DNA primase, DNA helicase, DNA ligase, and topoisomerase.
, Double stranded break (DSB) mechanisms for genome editing?
Select all that apply. Correct Answers Repair of breaks by
NHEJ can lead to gene inactivation.
+
The addition of foreign DNA can lead to specific gene
modifications.
double stranded breaks (DSB)? Correct Answers DSB repair is
handled by the same factors involved in homologous
recombination during meiosis
DSB repair pathways were likely essential before genome sizes
could increase in an organism
Mutations in genes involved in DSB repair can lead to cancer
double stranded breaks REPAIR by non-homologous end
joining is more likely to occur outside of S phase and G2 than
homologous recombination. Why is this? Correct Answers
Outside of S phase and G2, there is no homologous chromosome
that the homologous recombination pathway can use for repair.
Double-stranded break repair REPAIR DAMAGED
TEMPLATE ENCOUNTER Correct Answers The replication
fork encounters a lesion at which repair has been initiated and
the fork collapses.
end replication problem more protect which cells? Select all that
apply. Correct Answers germ cancer stem
