GMS 6504 EVALUATION EXAM 2026 QUESTIONS AND
ANSWERS SCORED A+
✔✔addition of an inverse agonist to a system containing a receptor that does not
spontaneously activate will have what effect on the measured response in the absence
of any other ligand?
•increase
•decrease
•have no effect - ✔✔have no effect
✔✔you use an agonist to treat two systems that are identical other than the number of
receptors present. system A required 1 mM of the ligand to reach 100% response while
system B required 100 mM. which system has the larger receptor reserve?
•system A
•system B - ✔✔system A
✔✔the cellular veil ___ the measured response.
•always increased
•always decreases
•may increase or decrease - ✔✔may increase or decrease
✔✔assuming cellular amplification, measuring a response that is ___ the ligand binding
event will result in a larger measured response.
•closer to
•further from - ✔✔further from
✔✔when comparing two partial agonists, you are more likely to see a difference in
response if you are measuring a response that is ___ the ligand binding event. assume
that you are not limited in any way by the instrumentation used to measure the
response.
•closer to
•further from - ✔✔closer to
✔✔you treat a system with an agonist, wash out the agonist, and then add a second
treatment of the same agonist. your measurements show that the receptors underwent
desensitization due to pretreatment. the response you measured after the second
treatment must have been ___ the response measured after the first treatment.
•higher than
•lower than
, •the same as - ✔✔lower than
✔✔which binding assay technique does not require any additional steps after ligand
addition in order to eliminate non specific binding from the measured signal?
•radioactivity assay
•fluorescence assay
•polarization assay
•FRET assay - ✔✔FRET assay
✔✔if you double the agonist concentration, what will happen to the non specific
binding?
•stay the same
•double
•quadruple
•halve - ✔✔double
✔✔at what point after addition of an agonist does the rate of occupancy increase the
fastest?
•immediately after addition
•sometime between addition and reaching 100% occupancy
•right before reaching 100% occupancy - ✔✔immediately after addition
✔✔on a scatchard plot, 100% occupancy is reached when...
•X=0
•X=100
•Y=0
•Y=100 - ✔✔Y=0
✔✔you are carrying out a displacement binding assay. you find that, regardless of what
concentration of radioligand you start with, it always requires 50 mM of your cold ligand
to displace 50% of the radioligand and always requires 200 mM of your cold ligand to
completely displace the radioligand. your cold ligand is which type of antagonist?
•competitive
•noncompetitive
•allosteric - ✔✔noncompetitive
✔✔in a displacement binding experiment, a known allosteric antagonist will most likely
produce which effect?
•there is no concentration at which it will completely displace all of the radioligand
ANSWERS SCORED A+
✔✔addition of an inverse agonist to a system containing a receptor that does not
spontaneously activate will have what effect on the measured response in the absence
of any other ligand?
•increase
•decrease
•have no effect - ✔✔have no effect
✔✔you use an agonist to treat two systems that are identical other than the number of
receptors present. system A required 1 mM of the ligand to reach 100% response while
system B required 100 mM. which system has the larger receptor reserve?
•system A
•system B - ✔✔system A
✔✔the cellular veil ___ the measured response.
•always increased
•always decreases
•may increase or decrease - ✔✔may increase or decrease
✔✔assuming cellular amplification, measuring a response that is ___ the ligand binding
event will result in a larger measured response.
•closer to
•further from - ✔✔further from
✔✔when comparing two partial agonists, you are more likely to see a difference in
response if you are measuring a response that is ___ the ligand binding event. assume
that you are not limited in any way by the instrumentation used to measure the
response.
•closer to
•further from - ✔✔closer to
✔✔you treat a system with an agonist, wash out the agonist, and then add a second
treatment of the same agonist. your measurements show that the receptors underwent
desensitization due to pretreatment. the response you measured after the second
treatment must have been ___ the response measured after the first treatment.
•higher than
•lower than
, •the same as - ✔✔lower than
✔✔which binding assay technique does not require any additional steps after ligand
addition in order to eliminate non specific binding from the measured signal?
•radioactivity assay
•fluorescence assay
•polarization assay
•FRET assay - ✔✔FRET assay
✔✔if you double the agonist concentration, what will happen to the non specific
binding?
•stay the same
•double
•quadruple
•halve - ✔✔double
✔✔at what point after addition of an agonist does the rate of occupancy increase the
fastest?
•immediately after addition
•sometime between addition and reaching 100% occupancy
•right before reaching 100% occupancy - ✔✔immediately after addition
✔✔on a scatchard plot, 100% occupancy is reached when...
•X=0
•X=100
•Y=0
•Y=100 - ✔✔Y=0
✔✔you are carrying out a displacement binding assay. you find that, regardless of what
concentration of radioligand you start with, it always requires 50 mM of your cold ligand
to displace 50% of the radioligand and always requires 200 mM of your cold ligand to
completely displace the radioligand. your cold ligand is which type of antagonist?
•competitive
•noncompetitive
•allosteric - ✔✔noncompetitive
✔✔in a displacement binding experiment, a known allosteric antagonist will most likely
produce which effect?
•there is no concentration at which it will completely displace all of the radioligand
