MMSC490 UPPDATED EXAM STUDY GUIDE QUESTIONS
AND SOLUTIONS GUARANTEE A+
✔✔The first step in the formation of a transcription complex for mRNA transcription is
the binding of _______ to the TATA box.
a. TFIA
b. TFIIA
c. TFIIIA
d. TFIID - ✔✔d. TFIID
✔✔The large multi-subunit complex that links the general transcription factors to the
gene- specific transcription factors is called
a. the transcription complex.
b. Mediator.
c. the operator.
d. TBP. - ✔✔b. Mediator.
✔✔Release of RNA polymerase II to initiate transcription appears to be the direct result
of the
a. binding of TAFs to the polymerase.
b. unwinding of the DNA by helicases.
c. phosphorylation of RNA polymerase by a protein kinase.
d. removal of the nucleosome occupying the promoter site. - ✔✔c. phosphorylation of
RNA polymerase by a protein kinase.
✔✔A major difference between eukaryotic and prokaryotic RNA polymerases is that
eukaryotic polymerases
a. use a set of transcription factors to bind to and initiate transcription.
b. use sigma () factors to initiate transcription.
c. start from promoters.
d. start from origins of replication. - ✔✔a. use a set of transcription factors to bind to and
initiate transcription.
✔✔Enhancers have been found in
a. eukaryotic cells.
b. prokaryotic cells.
c. yeast.
d. Both a and b - ✔✔d. Both a and b
✔✔The immunoglobulin enhancer has binding sites for _______ regulatory protein(s).
a. one specific
b. multiple positive- and negative-acting
c. multiple positive-acting
d. multiple negative-acting - ✔✔b. multiple positive- and negative-acting
, ✔✔DNA sequences that prevent enhancers from acting on promoters located in
adjacent domains are called
a. insulators.
b. isolators.
c. repressors.
d. boundaries. - ✔✔a. insulators.
✔✔Kadonaga and Tjian were able to isolate the transcription factor Sp1 by exploiting its
ability to bind
a. the sequence TATA.
b. the sequence AAAUAAA.
c. the sequence GGGCGG.
d. RNA polymerase. - ✔✔c. the sequence GGGCGG.
✔✔Helix-turn-helix transcription factor proteins are found
a. only in insects.
b. only in eukaryotes.
c. only in prokaryotes.
d. in both prokaryotes and eukaryotes. - ✔✔d. in both prokaryotes and eukaryotes.
✔✔Eukaryotic gene repressor proteins are thought to act by binding to
a. DNA sites in competition with activating proteins.
b. specific activating proteins, preventing their binding to DNA.
c. basal transcription factors, inhibiting transcription.
d. All of the above - ✔✔d. All of the above
✔✔DNA methylation patterns and their resulting genetic imprinting are inherited by
which of the following mechanisms?
a. Enzymes put a methyl group on cytosine residues of newly replicated CpG
sequences across from G-methyl-C sequences.
b. Methyl-CTP is incorporated into DNA during replication only across from a G- methyl-
C sequence.
c. When its gene is activated, a methyl group is added to certain CpG sequences in the
promoter region.
d. Methylation of genes only located on the X chromosome - ✔✔a. Enzymes put a
methyl group on cytosine residues of newly replicated CpG sequences across from G-
methyl-C sequences.
✔✔DNA methylation patterns of active genes tend to differ from those of inactive genes
in that the active genes have
a. more methylated nucleotides in their protein-coding regions.
b. more methylated nucleotides in their promoter regions.
c. fewer methylated nucleotides in their promoter regions.
d. Both a and b - ✔✔c. fewer methylated nucleotides in their promoter regions.
AND SOLUTIONS GUARANTEE A+
✔✔The first step in the formation of a transcription complex for mRNA transcription is
the binding of _______ to the TATA box.
a. TFIA
b. TFIIA
c. TFIIIA
d. TFIID - ✔✔d. TFIID
✔✔The large multi-subunit complex that links the general transcription factors to the
gene- specific transcription factors is called
a. the transcription complex.
b. Mediator.
c. the operator.
d. TBP. - ✔✔b. Mediator.
✔✔Release of RNA polymerase II to initiate transcription appears to be the direct result
of the
a. binding of TAFs to the polymerase.
b. unwinding of the DNA by helicases.
c. phosphorylation of RNA polymerase by a protein kinase.
d. removal of the nucleosome occupying the promoter site. - ✔✔c. phosphorylation of
RNA polymerase by a protein kinase.
✔✔A major difference between eukaryotic and prokaryotic RNA polymerases is that
eukaryotic polymerases
a. use a set of transcription factors to bind to and initiate transcription.
b. use sigma () factors to initiate transcription.
c. start from promoters.
d. start from origins of replication. - ✔✔a. use a set of transcription factors to bind to and
initiate transcription.
✔✔Enhancers have been found in
a. eukaryotic cells.
b. prokaryotic cells.
c. yeast.
d. Both a and b - ✔✔d. Both a and b
✔✔The immunoglobulin enhancer has binding sites for _______ regulatory protein(s).
a. one specific
b. multiple positive- and negative-acting
c. multiple positive-acting
d. multiple negative-acting - ✔✔b. multiple positive- and negative-acting
, ✔✔DNA sequences that prevent enhancers from acting on promoters located in
adjacent domains are called
a. insulators.
b. isolators.
c. repressors.
d. boundaries. - ✔✔a. insulators.
✔✔Kadonaga and Tjian were able to isolate the transcription factor Sp1 by exploiting its
ability to bind
a. the sequence TATA.
b. the sequence AAAUAAA.
c. the sequence GGGCGG.
d. RNA polymerase. - ✔✔c. the sequence GGGCGG.
✔✔Helix-turn-helix transcription factor proteins are found
a. only in insects.
b. only in eukaryotes.
c. only in prokaryotes.
d. in both prokaryotes and eukaryotes. - ✔✔d. in both prokaryotes and eukaryotes.
✔✔Eukaryotic gene repressor proteins are thought to act by binding to
a. DNA sites in competition with activating proteins.
b. specific activating proteins, preventing their binding to DNA.
c. basal transcription factors, inhibiting transcription.
d. All of the above - ✔✔d. All of the above
✔✔DNA methylation patterns and their resulting genetic imprinting are inherited by
which of the following mechanisms?
a. Enzymes put a methyl group on cytosine residues of newly replicated CpG
sequences across from G-methyl-C sequences.
b. Methyl-CTP is incorporated into DNA during replication only across from a G- methyl-
C sequence.
c. When its gene is activated, a methyl group is added to certain CpG sequences in the
promoter region.
d. Methylation of genes only located on the X chromosome - ✔✔a. Enzymes put a
methyl group on cytosine residues of newly replicated CpG sequences across from G-
methyl-C sequences.
✔✔DNA methylation patterns of active genes tend to differ from those of inactive genes
in that the active genes have
a. more methylated nucleotides in their protein-coding regions.
b. more methylated nucleotides in their promoter regions.
c. fewer methylated nucleotides in their promoter regions.
d. Both a and b - ✔✔c. fewer methylated nucleotides in their promoter regions.
