ASCP MLT EXAM COMPREHENSIVE TEST
PAPER 2026 COMPLETE SOLUTIONS
◉ A;
Oxalate, EDTA, and citrate are anticoagulants that inhibit clot
formation. Answer: Which of the following blood additives is most
useful for serum collection:
A. Polymer barrier
B. Oxalate
C. EDTA
D. Citrate
◉ B;
This patient is most likely suffering from an immediate-acting
coagulation inhibitor; most commonly, lupus anticoagulant. Notice
that the addition of normal pooled plasma does not correct upon
initial or incubated mix, which means that the inhibitor is not time
or temperature-dependent.
Factor VIII is not the correct answer as a factor deficiency would
have corrected upon the addition of normal pooled plasma. Factor
VII is not the correct answer, as the aPTT assay does not account for
factor VII activity or concentration. Answer: The laboratorian
,completed the mixing study ordered for John Doe. The results are as
follows:
Initial aPTT result: 167 seconds
Initial 1:1 Mix with Normal Pooled Plasma: 158 seconds
Incubated 1:1 Mix with Normal Pooled Plasma: 150 seconds
Which of the choices below would most likely explain the results for
this patient?
A. Factor VIII deficiency
B. Immediate-acting coagulation inhibitor
C. Time/temperature-dependent coagulation inhibitor
D. Factor VII deficiency
◉ A;
HbsAg is positive in acute and chronic Hepatitis B infections, since
the antigen is found on the actual surface of the virus. HbeAg is
present in the blood when the hepatitis B viruses are replicating,
indicating an active infection. Anti-Hbc IgM is present due to the
immune response to the presence of the hepatitis core antigen and
indicates an acute infection. Anti-HBs is generally interpreted as
indicating recovery and immunity from hepatitis B virus infection,
,according to the CDC. Answer: Given the following results, what is
the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative
A. acute infection
B. chronic infection
C. immunization
D. susceptible
◉ A;
Albumin is a "negative" acute phase protein since it is found in
decreased levels during acute phase response. Alpha-1-antitrypsin,
fibrinogen, and ceruloplasmin are all "positive" acute phase proteins
that are found in increased levels during acute phase response.
Answer: Which one of the following usually shows a decrease during
an acute phase response?
A. Albumin
B. Alpha-1 Antitrypsin
, C. Fibrinogen
D. Ceruloplasmin
◉ False
Because lower titers could be due to both passive and immune anti-
D, in the absence of results that suggest immune anti-D, routine
antibody titration is not a good use of time compared to assuming
that anti-D is passive.
Best practice guidelines do NOT recommend routine titration for
women known to be injected with RhIg and exhibiting a 2+ or less
reaction with D+ red cells consistent with passive anti-D from RhIg.
Answer: True/False
A pregnant female who received RhIg at 28 weeks gestation has a
positive antibody screen at delivery. If the antibody has been
confirmed as anti-D alone and reacts 1+ in the indirect antiglobulin
test with D+ red cells, performing a titration to investigate if the
anti-D is immune is good practice.
◉ 1. B
2. D
3. A
4. C
PAPER 2026 COMPLETE SOLUTIONS
◉ A;
Oxalate, EDTA, and citrate are anticoagulants that inhibit clot
formation. Answer: Which of the following blood additives is most
useful for serum collection:
A. Polymer barrier
B. Oxalate
C. EDTA
D. Citrate
◉ B;
This patient is most likely suffering from an immediate-acting
coagulation inhibitor; most commonly, lupus anticoagulant. Notice
that the addition of normal pooled plasma does not correct upon
initial or incubated mix, which means that the inhibitor is not time
or temperature-dependent.
Factor VIII is not the correct answer as a factor deficiency would
have corrected upon the addition of normal pooled plasma. Factor
VII is not the correct answer, as the aPTT assay does not account for
factor VII activity or concentration. Answer: The laboratorian
,completed the mixing study ordered for John Doe. The results are as
follows:
Initial aPTT result: 167 seconds
Initial 1:1 Mix with Normal Pooled Plasma: 158 seconds
Incubated 1:1 Mix with Normal Pooled Plasma: 150 seconds
Which of the choices below would most likely explain the results for
this patient?
A. Factor VIII deficiency
B. Immediate-acting coagulation inhibitor
C. Time/temperature-dependent coagulation inhibitor
D. Factor VII deficiency
◉ A;
HbsAg is positive in acute and chronic Hepatitis B infections, since
the antigen is found on the actual surface of the virus. HbeAg is
present in the blood when the hepatitis B viruses are replicating,
indicating an active infection. Anti-Hbc IgM is present due to the
immune response to the presence of the hepatitis core antigen and
indicates an acute infection. Anti-HBs is generally interpreted as
indicating recovery and immunity from hepatitis B virus infection,
,according to the CDC. Answer: Given the following results, what is
the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative
A. acute infection
B. chronic infection
C. immunization
D. susceptible
◉ A;
Albumin is a "negative" acute phase protein since it is found in
decreased levels during acute phase response. Alpha-1-antitrypsin,
fibrinogen, and ceruloplasmin are all "positive" acute phase proteins
that are found in increased levels during acute phase response.
Answer: Which one of the following usually shows a decrease during
an acute phase response?
A. Albumin
B. Alpha-1 Antitrypsin
, C. Fibrinogen
D. Ceruloplasmin
◉ False
Because lower titers could be due to both passive and immune anti-
D, in the absence of results that suggest immune anti-D, routine
antibody titration is not a good use of time compared to assuming
that anti-D is passive.
Best practice guidelines do NOT recommend routine titration for
women known to be injected with RhIg and exhibiting a 2+ or less
reaction with D+ red cells consistent with passive anti-D from RhIg.
Answer: True/False
A pregnant female who received RhIg at 28 weeks gestation has a
positive antibody screen at delivery. If the antibody has been
confirmed as anti-D alone and reacts 1+ in the indirect antiglobulin
test with D+ red cells, performing a titration to investigate if the
anti-D is immune is good practice.
◉ 1. B
2. D
3. A
4. C
